ChemInform Abstract: A Facile New Synthesis of Aldehyde Enamines in High Yield and High Purity.

ChemInform ◽  
2010 ◽  
Vol 25 (49) ◽  
pp. no-no
Author(s):  
G. B. FISHER ◽  
L. LEE ◽  
F. W. KLETTKE
Keyword(s):  
High Purity ◽  
High Yield ◽  
New Synthesis

A Facile New Synthesis of Aldehyde Enamines in High Yield and High Purity

1994 ◽  
Vol 24 (11) ◽  
pp. 1541-1546 ◽  
Author(s):  
Gary B. Fisher ◽  
Lawrence Lee ◽  
Frederick W. Klettke
Keyword(s):  
High Purity ◽  
High Yield ◽  
New Synthesis

A High-Yield, High-Purity Elutriation Method for Preparing Human Granulocytes Demonstrating Enhanced Experimental Lifetimes

1987 ◽  
Vol 42 (1) ◽  
pp. 43-50 ◽  
Author(s):  
James O. Tolley ◽  
Geneva M. Omann ◽  
Algirdas J. Jesaitis
Keyword(s):  
High Purity ◽  
High Yield ◽  
Human Granulocytes

scholarly journals A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2

2020 ◽  
Author(s):  
Cecy Xi ◽  
Arianna Arianna Di Fazio ◽  
Naveed Nadvi ◽  
Karishma Patel ◽  
Michelle Xiang ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
High Purity ◽  
Specific Activity ◽  
Specific Binding ◽  
Exchange Chromatography ◽  
Cell Surface Receptor ◽  
Spike Protein ◽  
High Yield ◽  
Purification Procedure ◽  
Dipeptidyl Peptidase 4

Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulfate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor binding domain (RBD) were measured using surface plasmon resonance. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.

Flavan derivatives. XXIII. Preparation and synthetic applications of Flav-3-enes

1968 ◽  
Vol 21 (9) ◽  
pp. 2247 ◽  
Author(s):  
JW Clark-Lewis ◽  
RW Jemison
Keyword(s):  
Sodium Borohydride ◽  
Acidic Medium ◽  
Catalytic Reduction ◽  
Heterocyclic Ring ◽  
High Yield ◽  
Lithium Aluminium Hydride ◽  
Lower Field ◽  
Field Characteristic ◽  
Lithium Aluminium ◽  
New Synthesis

2'-Hydroxychalcones and α-alkoxy-2'-hydroxychalcones are converted by sodium borohydride in isopropanol into flav-3-enes and 3-alkoxyflav-3-enes in the convenient new synthesis which makes these flavenes readily available. Catalytic reduction of the flavenes gives the corresponding flavans or 3-alkoxyflavans in high yield, and the latter are obtained mainly in the 2,s-cis-form. The flavenes immediately give flavs lium cations in the cold when treated with acids in air, and oxidation of 5,7,3',4'-tetramethoxyflav-3-ene with benzoquinone in an acidic medium gave the flavylium salt, isolated as the ferrichloride. Reduction of 5,7,3',4'-tetramethoxy-flavylium chloride with lithium aluminium hydride gave 5,7,3',4'-tetramethoxy-flav-2-ene identical with the flavene obtained from (-)-epicatechin tetramethyl ether, and confirms an earlier investigation by Gramshaw, Johnson, and King. In its N.M.R. spectrum the heterocyclic-ring protons of this flav-2-ene give an ABX multiplet which is easily distinguished from the ABX multiplet at much lower field characteristic of flav-3-enes.

scholarly journals Water-based synthesis and cleaning methods for high purity ZnO nanoparticles – comparing acetate, chloride, sulphate and nitrate zinc salt precursors

RSC Advances ◽  
2014 ◽  
Vol 4 (67) ◽  
pp. 35568-35577 ◽  
Author(s):  
A. M. Pourrahimi ◽  
D. Liu ◽  
L. K. H. Pallon ◽  
R. L. Andersson ◽  
A. Martínez Abad ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
High Purity ◽  
Zinc Salt ◽  
High Yield ◽  
Water Based ◽  
Zinc Salts ◽  
Precipitation Synthesis ◽  
Cleaning Methods

The effect of using different zinc salts on size, morphology and photoluminescence of ZnO nanoparticles in high-yield aqueous precipitation synthesis.

A new one-pot synthesis of μ-oxo dimeric iron(III) porphyrins from meso-tetraarylporphyrins

2009 ◽  
Vol 13 (08n09) ◽  
pp. 854-858 ◽  
Author(s):  
Qiang Liu ◽  
Yan-Zhi Gong ◽  
Chang-Jun Gong ◽  
Qing-Hong Li ◽  
Can-Cheng Guo
Keyword(s):  
Synthetic Methods ◽  
High Yield ◽  
Reaction Intermediates ◽  
Ferrous Chloride ◽  
Iron Porphyrins ◽  
One Pot ◽  
Convenient Procedure ◽  
One Pot Synthesis ◽  
New Synthesis ◽  
The One

A new synthesis of μ-oxo dimeric iron(III) porphyrins from meso-tetraarylporphyrins in one-pot procedure is reported. μ-oxo dimeric iron(III) porphyrin was obtained in high yield (up to 93%) from the reaction of meso-tetraarylporphyrin with ferrous chloride in DMF at pH 8–11. Compared with other synthetic methods of μ-oxo dimeric iron(III) porphyrin from meso-tetraarylporphyrins, the one-pot procedure has higher yields of μ-oxo dimeric iron(III) porphyrins and is a simpler and more convenient procedure. In order to evaluate the range of applicability of this method, μ-oxo dimeric iron(III) porphyrins with different substituents were prepared by the reaction of the corresponding meso-tetraarylporphyrins with ferrous chloride. The results showed that it was possible to apply this one-pot procedure to the synthesis of other μ-oxo dimeric iron porphyrins in excellent yields. A mechanism for the formation of μ-oxo dimeric iron porphyrins was proposed based on the reaction intermediates characterized by HPLC and UV-vis methods.

Sign in / Sign up

Export Citation Format

Share Document