scholarly journals Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

2018 ◽  
Vol 25 (1) ◽  
pp. 195-199 ◽  
Author(s):  
Pablo G. Argudo ◽  
Mónica Carril ◽  
María T. Martín‐Romero ◽  
Juan J. Giner‐Casares ◽  
Carolina Carrillo‐Carrión
Keyword(s):  
Quantum Dots ◽  
Cellular Uptake ◽  
Surface Active

scholarly journals Quantum Dots as a Good Carriers of Unsymmetrical Bisacridines for Modulating Cellular Uptake and the Biological Response in Lung and Colon Cancer Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 462 ◽  
Author(s):  
Joanna Pilch ◽  
Patrycja Kowalik ◽  
Piotr Bujak ◽  
Anna M. Nowicka ◽  
Ewa Augustin
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Laser Scanning ◽  
Biological Response ◽  
Drug Carriers ◽  
Confocal Laser ◽  
Normal Cells ◽  
H460 Cells ◽  
Hct116 Cells

Nanotechnology-based drug delivery provides a promising area for improving the efficacy of cancer treatments. Therefore, we investigate the potential of using quantum dots (QDs) as drug carriers for antitumor unsymmetrical bisacridine derivatives (UAs) to cancer cells. We examine the influence of QD–UA hybrids on the cellular uptake, internalization (Confocal Laser Scanning Microscope), and the biological response (flow cytometry and light microscopy) in lung H460 and colon HCT116 cancer cells. We show the time-dependent cellular uptake of QD–UA hybrids, which were more efficiently retained inside the cells compared to UAs alone, especially in H460 cells, which could be due to multiple endocytosis pathways. In contrast, in HCT116 cells, the hybrids were taken up only by one endocytosis mechanism. Both UAs and their hybrids induced apoptosis in H460 and HCT116 cells (to a greater extent in H460). Cells which did not die underwent senescence more efficiently following QDs–UAs treatment, compared to UAs alone. Cellular senescence was not observed in HCT116 cells following treatment with both UAs and their hybrids. Importantly, QDgreen/red themselves did not provoke toxic responses in cancer or normal cells. In conclusion, QDs are good candidates for targeted UA delivery carriers to cancer cells while protecting normal cells from toxic drug activities.

scholarly journals Diselenolane‐Mediated Cellular Uptake: Efficient Cytosolic Delivery of Probes, Peptides, Proteins, Artificial Metalloenzymes and Protein‐Coated Quantum Dots

2019 ◽  
Vol 25 (16) ◽  
pp. 4047-4051 ◽  
Author(s):  
Eline Bartolami ◽  
Dimitris Basagiannis ◽  
Lili Zong ◽  
Rémi Martinent ◽  
Yasunori Okamoto ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cellular Uptake ◽  
Cytosolic Delivery ◽  
Artificial Metalloenzymes

scholarly journals Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2250
Author(s):  
Shanmugavel Chinnathambi ◽  
Nobutaka Hanagata ◽  
Tomohiko Yamazaki ◽  
Naoto Shirahata
Keyword(s):  
Quantum Dots ◽  
Blood Plasma ◽  
Cellular Uptake ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Infrared Light ◽  
Spectral Window ◽  
Blood Plasma Proteins

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein’s secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellular uptake (NIH3T3 Fibroblasts) and minimal cytotoxicity using different cell lines (HeLa, A549, and NIH3T3). This study offers a basis to design QDs without altering the biomacromolecule’s original conformation with enabled cellular uptake with minimal cytotoxicity.

Versatile surface-active ionic liquid: construction of microemulsions and their applications in light harvesting

2020 ◽  
Vol 22 (15) ◽  
pp. 8157-8163
Author(s):  
Krishnaiah Damarla ◽  
Sanjay Mehra ◽  
Pratap Bahadur ◽  
Debes Ray ◽  
V. K. Aswal ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Ionic Liquid ◽  
Light Harvesting ◽  
Advanced Materials ◽  
Core Shell ◽  
Surface Active Ionic Liquid ◽  
Surface Active

This article outlines a sustainable method towards the synthesis of advanced materials such as core/shell Quantum Dots (QDs) and their in situ stabilization using microemulsions (MEs).

scholarly journals Non-specific cellular uptake of surface-functionalized quantum dots

2010 ◽  
Vol 21 (28) ◽  
pp. 285105 ◽  
Author(s):  
T A Kelf ◽  
V K A Sreenivasan ◽  
J Sun ◽  
E J Kim ◽  
E M Goldys ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cellular Uptake

scholarly journals Nona-Arginine Facilitates Delivery of Quantum Dots into Cells via Multiple Pathways

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Yi Xu ◽  
Betty Revon Liu ◽  
Han-Jung Lee ◽  
Katie B. Shannon ◽  
Jeffrey G. Winiarz ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cellular Uptake ◽  
Metabolic Inhibitors ◽  
Cellular Internalization ◽  
Caveolin 1 ◽  
Dependent Process ◽  
A Cell ◽  
Low Efficiency ◽  
Rnai Technique ◽  
Energy Dependent

Semiconductor quantum dots (QDs) have recently been used to deliver and monitor biomolecules, such as drugs and proteins. However, QDs alone have a low efficiency of transport across the plasma membrane. In order to increase the efficiency, we used synthetic nona-arginine (SR9), a cell-penetrating peptide, to facilitate uptake. We found that SR9 increased the cellular uptake of QDs in a noncovalent binding manner between QDs and SR9. Further, we investigated mechanisms of QD/SR9 cellular internalization. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/SR9, indicating that internalization is an energy-dependent process. Results from both the pathway inhibitors and the RNA interference (RNAi) technique suggest that cellular uptake of QD/SR9 is predominantly a lipid raft-dependent process mediated by macropinocytosis. However, involvement of clathrin and caveolin-1 proteins in transducing QD/SR9 across the membrane cannot be completely ruled out.

Comprehensive evaluation of the cytotoxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium by cellular uptake and oxidative stress

2017 ◽  
Vol 4 (10) ◽  
pp. 2018-2029 ◽  
Author(s):  
Liang Hu ◽  
Jia Wan ◽  
Guangming Zeng ◽  
Anwei Chen ◽  
Guiqiu Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Quantum Dots ◽  
Phanerochaete Chrysosporium ◽  
Cellular Uptake ◽  
Comprehensive Evaluation ◽  
Physiological Responses ◽  
And Oxidative Stress

The cellular uptake of QDs and the induced physiological responses in an organism are investigated.

Cellular uptake and subcellular localization of highly luminescent silica-coated CdSe quantum dots – In vitro and in vivo

2011 ◽  
Vol 357 (2) ◽  
pp. 366-371 ◽  
Author(s):  
M. Vibin ◽  
R. Vinayakan ◽  
Annie John ◽  
C.S. Rejiya ◽  
V. Raji ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Subcellular Localization ◽  
Cellular Uptake ◽  
Cdse Quantum Dots
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