scholarly journals Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED

2017 ◽  
Vol 23 (50) ◽  
pp. 12114-12119 ◽  
Author(s):  
Alexey N. Butkevich ◽  
Vladimir N. Belov ◽  
Kirill Kolmakov ◽  
Viktor V. Sokolov ◽  
Heydar Shojaei ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Super Resolution ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

2019 ◽  
Author(s):  
Inbar Segal ◽  
Dikla Nachmias ◽  
Eyal Arbely ◽  
Natalie Elia
Keyword(s):  
Mammalian Cells ◽  
Fluorescent Dyes ◽  
Specific Protein ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cells ◽  
Bioorthogonal Labeling ◽  
Labeling Approach ◽  
Genetic Code Expansion ◽  
Live Cell Labeling

AbstractIn the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used live cell applications, partly because it needs to be adjusted to the specific protein under study. Here, we present a generic, 14-residues long, N-terminal tag for GCE-based labeling of proteins in live mammalian cells. Using this tag, we generated a library of GCE-based organelle markers, demonstrating the applicability of the tag for labeling a plethora of proteins and organelles. Finally, we show that the HA epitope, used as a backbone in our tag, can be substituted with other epitopes and, in some cases, can be completely removed, reducing the tag length to 5 residues. The GCE-tag presented here offers a powerful, easy-to-implement tool for live cell labeling of cellular proteins with small and bright probes.

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

2014 ◽  
Vol 53 (8) ◽  
pp. 2245-2249 ◽  
Author(s):  
Ivana Nikić ◽  
Tilman Plass ◽  
Oliver Schraidt ◽  
Jędrzej Szymański ◽  
John A. G. Briggs ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Cell Labeling ◽  
Dual Color ◽  
Live Cell Labeling ◽  
Super Resolution Microscopy

scholarly journals A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging

2021 ◽  
Vol 8 ◽  
Author(s):  
Yubing Han ◽  
Zhimin Zhang ◽  
Wenjie Liu ◽  
Yuanfa Yao ◽  
Yingke Xu ◽  
...  
Keyword(s):  
Super Resolution ◽  
Fluorescent Labeling ◽  
Live Cell ◽  
Cell Labeling ◽  
Cell Permeability ◽  
Biological Research ◽  
Cell Penetration ◽  
Mitochondrial Marker ◽  
Live Cell Labeling

Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.

scholarly journals A Trojan Horse for live-cell super-resolution microscopy

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gerti Beliu ◽  
Markus Sauer
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Trojan Horse ◽  
Live Cell ◽  
Cell Labeling ◽  
Intracellular Organelles ◽  
Live Cell Labeling ◽  
Super Resolution Microscopy ◽  
The Way

AbstractNew peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation, paving the way for refined multicolor super-resolution fluorescence imaging.

Frontispiece: Highly Stabletrans-Cyclooctene Amino Acids for Live-Cell Labeling

2015 ◽  
Vol 21 (35) ◽  
pp. n/a-n/a
Author(s):  
Jan-Erik Hoffmann ◽  
Tilman Plass ◽  
Ivana Nikić ◽  
Iker Valle Aramburu ◽  
Christine Koehler ◽  
...  
Keyword(s):  
Amino Acids ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Tetrazine-Containing Amino Acid for Peptide Modification and Live Cell Labeling

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0141918 ◽  
Author(s):  
Zhongqiu Ni ◽  
Lanxia Zhou ◽  
Xu Li ◽  
Jing Zhang ◽  
Shouliang Dong
Keyword(s):  
Amino Acid ◽  
Live Cell ◽  
Cell Labeling ◽  
Peptide Modification ◽  
Live Cell Labeling

scholarly journals A zeolite-based ship-in-a-bottle route to ultrasmall carbon dots for live cell labeling and bioimaging

2020 ◽  
Vol 2 (12) ◽  
pp. 5803-5809
Author(s):  
Lei Dong ◽  
Dehong Hu ◽  
Yanding Wang ◽  
Zonghai Sheng ◽  
Mei Hong ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Live Cell ◽  
Cell Labeling ◽  
Excellent Performance ◽  
Live Cell Labeling

The smallest-pore SAPO-20 zeolite confined pyrolysis of organics afforded ultrasmall uniform carbon dots with excellent performance in bioimaging.

Intracellular Delivery of Quantum Dots for Live Cell Labeling and Organelle Tracking

2004 ◽  
Vol 16 (12) ◽  
pp. 961-966 ◽  
Author(s):  
A. M. Derfus ◽  
W. C. W. Chan ◽  
S. N. Bhatia
Keyword(s):  
Quantum Dots ◽  
Intracellular Delivery ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Development of SNAP-tag-mediated live cell labeling as an alternative to GFP inPorphyromonas gingivalis

2010 ◽  
Vol 59 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Ophélie Nicolle ◽  
Astrid Rouillon ◽  
Helene Guyodo ◽  
Zohreh Tamanai-Shacoori ◽  
Fatiha Chandad ◽  
...  
Keyword(s):  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals Live Cell Labeling with Terpyridine Derivative Proligands to Measure Cytotoxicity Mediated by Immune Cells

ChemMedChem ◽  
2017 ◽  
Vol 12 (23) ◽  
pp. 2006-2013 ◽  
Author(s):  
Yuki Sakai ◽  
Satoshi Mizuta ◽  
Asuka Kumagai ◽  
Mohammed S. O. Tagod ◽  
Hiroaki Senju ◽  
...  
Keyword(s):  
Immune Cells ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling
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