Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

2014 ◽  
Vol 53 (8) ◽  
pp. 2245-2249 ◽  
Author(s):  
Ivana Nikić ◽  
Tilman Plass ◽  
Oliver Schraidt ◽  
Jędrzej Szymański ◽  
John A. G. Briggs ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Cell Labeling ◽  
Dual Color ◽  
Live Cell Labeling ◽  
Super Resolution Microscopy

scholarly journals A Trojan Horse for live-cell super-resolution microscopy

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gerti Beliu ◽  
Markus Sauer
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Trojan Horse ◽  
Live Cell ◽  
Cell Labeling ◽  
Intracellular Organelles ◽  
Live Cell Labeling ◽  
Super Resolution Microscopy ◽  
The Way

AbstractNew peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation, paving the way for refined multicolor super-resolution fluorescence imaging.

scholarly journals Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED

2017 ◽  
Vol 23 (50) ◽  
pp. 12114-12119 ◽  
Author(s):  
Alexey N. Butkevich ◽  
Vladimir N. Belov ◽  
Kirill Kolmakov ◽  
Viktor V. Sokolov ◽  
Heydar Shojaei ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Super Resolution ◽  
Live Cell ◽  
Cell Labeling ◽  
Live Cell Labeling

scholarly journals A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging

2021 ◽  
Vol 8 ◽  
Author(s):  
Yubing Han ◽  
Zhimin Zhang ◽  
Wenjie Liu ◽  
Yuanfa Yao ◽  
Yingke Xu ◽  
...  
Keyword(s):  
Super Resolution ◽  
Fluorescent Labeling ◽  
Live Cell ◽  
Cell Labeling ◽  
Cell Permeability ◽  
Biological Research ◽  
Cell Penetration ◽  
Mitochondrial Marker ◽  
Live Cell Labeling

Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.

scholarly journals Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grußmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Fluorogenic Probes ◽  
Super Resolution Microscopy

scholarly journals Ultrasmall, Bright, and Photostable Fluorescent Core–Shell Aluminosilicate Nanoparticles for Live‐Cell Optical Super‐Resolution Microscopy

2021 ◽  
Vol 33 (8) ◽  
pp. 2006829
Author(s):  
Jacob A. Erstling ◽  
Joshua A. Hinckley ◽  
Nirmalya Bag ◽  
Jessica Hersh ◽  
Grant B. Feuer ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Core Shell ◽  
Super Resolution Microscopy

Dual-color fluorescence emission difference super-resolution microscopy

2018 ◽  
Vol 11 (3) ◽  
pp. 329-336
Author(s):  
张智敏 ZHANG Zhi-min ◽  
匡翠方 KUANG Cui-fang ◽  
王子昂 WANG Zi-ang ◽  
朱大钊 ZHU Da-zhao ◽  
陈友华 CHEN You-hua ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Super Resolution ◽  
Dual Color ◽  
Super Resolution Microscopy

scholarly journals Live cell single molecule-guided Bayesian localization super resolution microscopy

Cell Research ◽  
2016 ◽  
Vol 27 (5) ◽  
pp. 713-716 ◽  
Author(s):  
Fan Xu ◽  
Mingshu Zhang ◽  
Wenting He ◽  
Renmin Han ◽  
Fudong Xue ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy
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