scholarly journals Effects of Geometric Isomerism and Anions on the Kinetics and Mechanism of the Stepwise Formation of Long-Range DNA Interstrand Cross-Links by Dinuclear Platinum Antitumor Complexes

2008 ◽  
Vol 14 (21) ◽  
pp. 6391-6405 ◽  
Author(s):  
Junyong Zhang ◽  
Donald S. Thomas ◽  
Susan J. Berners-Price ◽  
Nicholas Farrell
Keyword(s):  
Long Range ◽  
Kinetics And Mechanism ◽  
Geometric Isomerism ◽  
Dinuclear Platinum ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Antitumor Complexes ◽  
Stepwise Formation

Effects of geometric isomerism in dinuclear platinum antitumor complexes on aquation reactions in the presence of perchlorate, acetate and phosphate

2005 ◽  
Vol 10 (6) ◽  
pp. 652-666 ◽  
Author(s):  
Junyong Zhang ◽  
Donald S. Thomas ◽  
Murray S. Davies ◽  
Susan J. Berners-Price ◽  
Nicholas Farrell
Keyword(s):  
Geometric Isomerism ◽  
Dinuclear Platinum ◽  
Antitumor Complexes

Effect of Geometric Isomerism in Dinuclear Platinum Antitumor Complexes on DNA Interstrand Cross-Linking†

Biochemistry ◽  
1999 ◽  
Vol 38 (34) ◽  
pp. 10997-11005 ◽  
Author(s):  
Jana Kašpárková ◽  
Olga Nováková ◽  
Oldřich Vrána ◽  
Nicholas Farrell ◽  
Viktor Brabec
Keyword(s):  
Cross Linking ◽  
Geometric Isomerism ◽  
Dinuclear Platinum ◽  
Antitumor Complexes

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

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