A Smart Surface in a Microfluidic Chip for Controlled Protein Separation

2007 ◽  
Vol 13 (18) ◽  
pp. 5113-5120 ◽  
Author(s):  
Li Mu ◽  
Ying Liu ◽  
Shaoyu Cai ◽  
Jilie Kong
Keyword(s):  
Microfluidic Chip ◽  
Protein Separation ◽  
Smart Surface

“Click” chemistry-based surface modification of poly(dimethylsiloxane) for protein separation in a microfluidic chip

2010 ◽  
Vol 31 (18) ◽  
pp. 3129-3136 ◽  
Author(s):  
Zhaowei Zhang ◽  
Xiaojun Feng ◽  
Fei Xu ◽  
Xin Liu ◽  
Bi-Feng Liu
Keyword(s):  
Surface Modification ◽  
Click Chemistry ◽  
Microfluidic Chip ◽  
Protein Separation

Native Protein Separation by Isoelectric Focusing and Blue Gel Electrophoresis-Coupled Two-Dimensional Microfluidic Chip Electrophoresis

2014 ◽  
Vol 77 (19-20) ◽  
pp. 1339-1346 ◽  
Author(s):  
Ni Hou ◽  
Yu Chen ◽  
Shiyong Yu ◽  
Zongliang Quan ◽  
Chenhua Pan ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Gel Electrophoresis ◽  
Protein Separation ◽  
Two Dimensional ◽  
Native Protein

Drop driving on digital microfluidic chip

2020 ◽  
Vol 28 (11) ◽  
pp. 2488-2496
Author(s):  
Hong WANG ◽  
◽  
Jie ZHENG ◽  
Yan-peng YAN ◽  
Song WANG ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Digital Microfluidic

An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip

2013 ◽  
Vol 40 (11) ◽  
pp. 1668-1673
Author(s):  
Min DU ◽  
Xiong-Ying YE ◽  
Jin-Yang FENG ◽  
Zeng-Shuai MA ◽  
Zhao-Ying ZHOU
Keyword(s):  
Microfluidic Chip ◽  
Enrichment Method

An enzyme reactor based on aptamer modified microfluidic chip for protein analysis

2013 ◽  
Vol 30 (11) ◽  
pp. 1127-1132 ◽  
Author(s):  
Peng XIAO ◽  
Dalei LI ◽  
Yan MAN ◽  
Lina GENG ◽  
Xuefei LU ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Protein Analysis ◽  
Enzyme Reactor

scholarly journals Microfluidic Chip for Detection of Fungal Infections

ACS Omega ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 7474-7481 ◽  
Author(s):  
Waseem Asghar ◽  
Mazhar Sher ◽  
Nida S. Khan ◽  
Jatin M. Vyas ◽  
Utkan Demirci
Keyword(s):  
Microfluidic Chip ◽  
Fungal Infections

scholarly journals Computer-supported Detection of M-Components and Evaluation of Immunoglobulins after Capillary Electrophoresis

2001 ◽  
Vol 47 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Magnus Jonsson ◽  
Joyce Carlson ◽  
Jan-Olof Jeppsson ◽  
Per Simonsson
Keyword(s):  
Capillary Electrophoresis ◽  
Decision Support ◽  
Protein Separation ◽  
Serum Proteins ◽  
Cost Effective ◽  
Clinical Samples ◽  
Serum Samples ◽  
Computerized Decision Support ◽  
Cost Effective Method ◽  
Mathematical Algorithms

Abstract Background: Electrophoresis of serum samples allows detection of monoclonal gammopathies indicative of multiple myeloma, Waldenström macroglobulinemia, monoclonal gammopathy of undetermined significance, and amyloidosis. Present methods of high-resolution agarose gel electrophoresis (HRAGE) and immunofixation electrophoresis (IFE) are manual and labor-intensive. Capillary zone electrophoresis (CZE) allows rapid automated protein separation and produces digital absorbance data, appropriate as input for a computerized decision support system. Methods: Using the Beckman Paragon CZE 2000 instrument, we analyzed 711 routine clinical samples, including 95 monoclonal components (MCs) and 9 cases of Bence Jones myeloma, in both the CZE and HRAGE systems. Mathematical algorithms developed for the detection of monoclonal immunoglobulins (MCs) in the γ- and β-regions of the electropherogram were tested on the entire material. Additional algorithms evaluating oligoclonality and polyclonal concentrations of immunoglobulins were also tested. Results: CZE electropherograms corresponded well with HRAGE. Only one IgG MC of 1 g/L, visible on HRAGE, was not visible after CZE. Algorithms detected 94 of 95 MCs (98.9%) and 100% of those visible after CZE. Of 607 samples lacking an MC on HRAGE, only 3 were identified by the algorithms (specificity, 99%). Algorithms evaluating total gammaglobulinemia and oligoclonality also identified several cases of Bence Jones myeloma. Conclusions: The use of capillary electrophoresis provides a modern, rapid, and cost-effective method of analyzing serum proteins. The additional option of computerized decision support, which provides rapid and standardized interpretations, should increase the clinical availability and usefulness of protein analyses in the future.

scholarly journals An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 177 ◽  
Author(s):  
Zengming Zhang ◽  
Shuhao Zhao ◽  
Fei Hu ◽  
Guangpu Yang ◽  
Juan Li ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Cross Flow ◽  
Flow Channel ◽  
Isothermal Amplification ◽  
Fluorescent Detection ◽  
Lamp Method ◽  
Biological Studies ◽  
Micro Cavity ◽  
Integrated Device

The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption.

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

2011 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yuwadee Boonyasit ◽  
Thitima Maturos ◽  
Assawapong Sappat ◽  
Apichai Jomphoak ◽  
Adisorn Tuantranont ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Arsenazo Iii ◽  
Passive Micromixer
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