Immobilisation of Flavin-Adenine-Dinucleotide-Dependent Glucose Dehydrogenase α Subunit in Free-Standing Graphitised Carbon Nanofiber Paper Using a Bifunctional Cross-Linker for an Enzymatic Biofuel Cell

2014 ◽  
Vol 1 (11) ◽  
pp. 1844-1848 ◽  
Author(s):  
Deby Fapyane ◽  
Yooseok Lee ◽  
Chyi Yan Lim ◽  
Jou-Hyeon Ahn ◽  
Seon-Won Kim ◽  
...  
Keyword(s):  
Flavin Adenine Dinucleotide ◽  
Carbon Nanofiber ◽  
Glucose Dehydrogenase ◽  
Biofuel Cell ◽  
Free Standing ◽  
Α Subunit ◽  
Enzymatic Biofuel Cell ◽  
Cross Linker

Three-Dimensional Glucose/Oxygen Biofuel Cells Based on Enzymes Embedded in Tetrabutylammonium Modified Nafion

2021 ◽  
Vol 18 (4) ◽  
Author(s):  
Yuchen Hui ◽  
Xiaoyan Ma ◽  
Rong Cai ◽  
Shelley D. Minteer
Keyword(s):  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Tetrabutylammonium Bromide ◽  
Three Dimensional ◽  
Phosphate Buffer Solution ◽  
Open Circuit ◽  
Biofuel Cell ◽  
Buffer Solution ◽  
Multi Wall Carbon Nanotubes ◽  
Enzymatic Biofuel Cell

Abstract A stable three-dimensional glucose/oxygen enzymatic biofuel cell is fabricated based on the method of polymer encapsulation-based immobilization. And three-dimensional carbon felt is used as the substrate of the bio-electrode for increasing enzymatic loading density. Gold nanoparticles and multi-wall carbon nanotubes are employed to promote direct electron transfer and enhance conductivity and electron conduction rate of bio-electrodes. Glucose dehydrogenase and bilirubin oxidase are immobilized with tetrabutylammonium bromide (TBAB) modified Nafion, which enhances the stability of the bio-electrodes by the immobilization method. A membrane-free glucose/oxygen biofuel cell is assembled with a high open-circuit voltage of 0.85 V and a maximum power density of 21.9 ± 0.1 μW/cm2 in 0.1 M pH 7.0 phosphate buffer solution with 100 mM glucose and air saturation. And the biofuel cell shows high stability to the condition. After 60 days of periodic storage experiments, the performance of the enzymatic biofuel cell still maintained 90.3% of its electrochemical performance.

scholarly journals Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications

ACS Omega ◽  
2017 ◽  
Vol 2 (4) ◽  
pp. 1660-1665 ◽  
Author(s):  
Hisanori Iwasa ◽  
Atsunori Hiratsuka ◽  
Kenji Yokoyama ◽  
Hirotaka Uzawa ◽  
Kouhei Orihara ◽  
...  
Keyword(s):  
Flavin Adenine Dinucleotide ◽  
Glucose Dehydrogenase ◽  
Biofuel Cell ◽  
Talaromyces Emersonii

scholarly journals Flavin Adenine Dinucleotide-Dependent Halogenase XanH and Engineering of Multifunctional Fusion Halogenases

2020 ◽  
Vol 86 (18) ◽  
Author(s):  
Lingxin Kong ◽  
Qing Wang ◽  
Zixin Deng ◽  
Delin You
Keyword(s):  
Protein Engineering ◽  
Fusion Protein ◽  
Natural Product ◽  
Flavin Adenine Dinucleotide ◽  
High Efficiency ◽  
Genetic Manipulation ◽  
Good Alternative ◽  
Limited Information ◽  
Flavin Reductase ◽  
Free Standing

ABSTRACT Xantholipin (compound 1), a polycyclic xanthone antibiotic, exhibited strong antibacterial activities and showed potent cytotoxicity. The biosynthetic gene cluster of compound 1 has been identified in our previous work, and the construction of xanthone nucleus has been well demonstrated. However, limited information of the halogenation involved in compound 1 biosynthesis is available. In this study, based on the genetic manipulation and biochemical assay, we characterized XanH as an indispensable flavin adenine dinucleotide (FAD)-dependent halogenase (FDH) for the biosynthesis of compound 1. XanH was found to be a bifunctional protein capable of flavin reduction and chlorination and exclusively used the NADH. However, the reduced flavin could not be fully and effectively utilized, and the presence of an extra flavin reductase (FDR) and chemical-reducing agent could promote the halogenation. XanH accepted its natural free-standing substrate with angular fused polycyclic aromatic systems. Meanwhile, it exhibited moderate halogenation activity and possessed high substrate specificity. The requirement of extra FDR for higher halogenation activity is tedious for future engineering. To facilitate efforts in engineering XanH derivative proteins, we constructed the self-sufficient FDR-XanH fusion proteins. The fusion protein E1 with comparable activities to that of XanH could be used as a good alternative for future protein engineering. Taken together, these findings reported here not only improve the understanding of polycyclic xanthones biosynthesis but also expand the substrate scope of FDH and pave the way for future engineering of biocatalysts for new active substance synthesis. IMPORTANCE Halogenation is important in medicinal chemistry and plays an essential role in the biosynthesis of active secondary metabolites. Halogenases have evolved to catalyze reactions with high efficiency and selectivity, and engineering efforts have been made to engage the selective reactivity in natural product biosynthesis. The enzymatic halogenations are an environmentally friendly approach with high regio- and stereoselectivity, which make it a potential complement to organic synthesis. FDHs constitute one of the most extensively elucidated class of halogenases; however, the inventory awaits to be expanded for biotechnology applications and for the generation of halogenated natural product analogues. In this study, XanH was found to reduce flavin and halogenated the freely diffusing natural substrate with an angular fused hexacyclic scaffold, findings which were different from those for the exclusively studied FDHs. Moreover, the FDR-XanH fusion protein E1 with comparable reactivity to that of XanH serves as a successful example of genetic fusions and sets an important stage for future protein engineering.

scholarly journals A Review of Electrospun Carbon Nanofiber-Based Negative Electrode Materials for Supercapacitors

Electrochem ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 236-250
Author(s):  
Arjun Prasad Tiwari ◽  
Tanka Mukhiya ◽  
Alagan Muthurasu ◽  
Kisan Chhetri ◽  
Minju Lee ◽  
...  
Keyword(s):  
Energy Storage ◽  
Electrode Materials ◽  
Carbon Nanofiber ◽  
Negative Electrode ◽  
Smart Devices ◽  
Potential Candidate ◽  
Free Standing ◽  
High Capacitance ◽  
Negative Electrode Materials ◽  
Carbon Based

The development of smart negative electrode materials with high capacitance for the uses in supercapacitors remains challenging. Although several types of electrode materials with high capacitance in energy storage have been reported, carbon-based materials are the most reliable electrodes due to their high conductivity, high power density, and excellent stability. The most common complaint about general carbon materials is that these electrode materials can hardly ever be used as free-standing electrodes. Free-standing carbon-based electrodes are in high demand and are a passionate topic of energy storage research. Electrospun nanofibers are a potential candidate to fill this gap. However, the as-spun carbon nanofibers (ECNFs) have low capacitance and low energy density on their own. To overcome the limitations of pure CNFs, increasing surface area, heteroatom doping and metal doping have been chosen. In this review, we introduce the negative electrode materials that have been developed so far. Moreover, this review focuses on the advances of electrospun nanofiber-based negative electrode materials and their limitations. We put forth a future perspective on how these limitations can be overcome to meet the demands of next-generation smart devices.

Design of a novel photoelectrochemical enzymatic biofuel cell with high power output under visible light

2021 ◽  
pp. 134037
Author(s):  
Xinzhou Huang ◽  
Long Ren ◽  
Chunyun Jiang ◽  
Xiangxiang Han ◽  
Xiaoshuang Yin ◽  
...  
Keyword(s):  
Visible Light ◽  
High Power ◽  
Power Output ◽  
Biofuel Cell ◽  
Enzymatic Biofuel Cell ◽  
High Power Output

Chemical deposition of MnO2 nanosheets on graphene-carbon nanofiber paper as free-standing and flexible electrode for supercapacitors

Ionics ◽  
2016 ◽  
Vol 22 (7) ◽  
pp. 1185-1195 ◽  
Author(s):  
Yuanzhan Wu ◽  
Suqin Liu ◽  
Kuangmin Zhao ◽  
Zhen He ◽  
Hao Yuan ◽  
...  
Keyword(s):  
Carbon Nanofiber ◽  
Chemical Deposition ◽  
Free Standing ◽  
Flexible Electrode ◽  
Mno2 Nanosheets
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