Three-Dimensional Glucose/Oxygen Biofuel Cells Based on Enzymes Embedded in Tetrabutylammonium Modified Nafion

2021 ◽  
Vol 18 (4) ◽  
Author(s):  
Yuchen Hui ◽  
Xiaoyan Ma ◽  
Rong Cai ◽  
Shelley D. Minteer
Keyword(s):  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Tetrabutylammonium Bromide ◽  
Three Dimensional ◽  
Phosphate Buffer Solution ◽  
Open Circuit ◽  
Biofuel Cell ◽  
Buffer Solution ◽  
Multi Wall Carbon Nanotubes ◽  
Enzymatic Biofuel Cell

Abstract A stable three-dimensional glucose/oxygen enzymatic biofuel cell is fabricated based on the method of polymer encapsulation-based immobilization. And three-dimensional carbon felt is used as the substrate of the bio-electrode for increasing enzymatic loading density. Gold nanoparticles and multi-wall carbon nanotubes are employed to promote direct electron transfer and enhance conductivity and electron conduction rate of bio-electrodes. Glucose dehydrogenase and bilirubin oxidase are immobilized with tetrabutylammonium bromide (TBAB) modified Nafion, which enhances the stability of the bio-electrodes by the immobilization method. A membrane-free glucose/oxygen biofuel cell is assembled with a high open-circuit voltage of 0.85 V and a maximum power density of 21.9 ± 0.1 μW/cm2 in 0.1 M pH 7.0 phosphate buffer solution with 100 mM glucose and air saturation. And the biofuel cell shows high stability to the condition. After 60 days of periodic storage experiments, the performance of the enzymatic biofuel cell still maintained 90.3% of its electrochemical performance.

scholarly journals Enhancement of Electrochemical Performance of Bilirubin Oxidase Modified Gas Diffusion Biocathode by Porphyrin Precursor

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Erica Pinchon ◽  
Mary Arugula ◽  
Kapil Pant ◽  
Sameer Singhal
Keyword(s):  
Electron Transfer ◽  
Oxygen Reduction ◽  
Power Density ◽  
Current Density ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Phosphate Buffer Solution ◽  
Gas Diffusion ◽  
Biofuel Cell ◽  
Bilirubin Oxidase

Recent studies have focused on tailoring the catalytic currents of multicopper oxidase (MCO) enzymes-based biocathodes to enhance oxygen reduction. Biocathodes modified with natural substrates specific for MCO enzymes demonstrated drastic improvement for oxygen reduction. Performance of 1-pyrenebutanoic acid, succinimidyl ester (PBSE), and 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (Di-Carb) oriented bilirubin oxidase (BOx) modified gas diffusion biocathode has been highly improved by incorporating hematin, a porphyrin precursor as electron transfer enhancement moiety. Hematin modified electrodes demonstrated direct electron transfer reaction of BOx exhibiting larger O2 reduction in current density in phosphate buffer solution (pH 7.0) without the need of a mediator. A remarkable improvement in the catalytic currents with 2.5-fold increase compared to non-hematin modified oriented BOx electrodes was achieved. Moreover, a mediatorless and compartmentless glucose/O2 biofuel cell based on DET-type bioelectrocatalysis via the BOx cathode and the glucose dehydrogenase (GDH) anode demonstrated peak power densities of 1 mW/cm2 at pH 7.0 with 100 mM glucose/10 mM NAD fuel. The maximum current density of 1.6 mA/cm2 and the maximum power density of 0.4 mW/cm2 were achieved at 300 mV with nonmodified BOx cathode, while 3.5 mA/cm2 and 1.1 mW/cm2 of current and power density were achieved with hematin modified cathode. The performance improved 2.4 times which attributes to the hematin acting as a natural precursor and activator for BOx activity enhancement.

scholarly journals Multi-Substrate Biofuel Cell Utilizing Glucose, Fructose and Sucrose as the Anode Fuels

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1534 ◽  
Author(s):  
Michał Kizling ◽  
Maciej Dzwonek ◽  
Anna Nowak ◽  
Łukasz Tymecki ◽  
Krzysztof Stolarczyk ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fuel Cells ◽  
Fuel Cell ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Three Dimensional ◽  
Modified Electrodes ◽  
Biofuel Cell ◽  
Capacitive Properties ◽  
A Cell

A significant problem still exists with the low power output and durability of the bioelectrochemical fuel cells. We constructed a fuel cell with an enzymatic cascade at the anode for efficient energy conversion. The construction involved fabrication of the flow-through cell by three-dimensional printing. Gold nanoparticles with covalently bound naphthoquinone moieties deposited on cellulose/polypyrrole (CPPy) paper allowed us to significantly improve the catalysis rate, both at the anode and cathode of the fuel cell. The enzymatic cascade on the anode consisted of invertase, mutarotase, Flavine Adenine Dinucleotide (FAD)-dependent glucose dehydrogenase and fructose dehydrogenase. The multi-substrate anode utilized glucose, fructose, sucrose, or a combination of them, as the anode fuel and molecular oxygen were the oxidant at the laccase-based cathode. Laccase was adsorbed on the same type of naphthoquinone modified gold nanoparticles. Interestingly, the naphthoquinone modified gold nanoparticles acted as the enzyme orienting units and not as mediators since the catalyzed oxygen reduction occurred at the potential where direct electron transfer takes place. Thanks to the good catalytic and capacitive properties of the modified electrodes, the power density of the sucrose/oxygen enzymatic fuel cells (EFC) reached 0.81 mW cm−2, which is beneficial for a cell composed of a single cathode and anode.

A novel three-dimensional carbonized PANI1600@CNTs network for enhanced enzymatic biofuel cell

2018 ◽  
Vol 101 ◽  
pp. 60-65 ◽  
Author(s):  
Zepeng Kang ◽  
Kailong Jiao ◽  
Jin Cheng ◽  
Ruiyun Peng ◽  
Shuqiang Jiao ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Biofuel Cell ◽  
Enzymatic Biofuel Cell

High-performance enzymatic biofuel cell based on three-dimensional graphene

2019 ◽  
Vol 44 (57) ◽  
pp. 30367-30374 ◽  
Author(s):  
Arman Amani Babadi ◽  
Wan Abd Al Qadr Imad Wan-Mohtar ◽  
Jo-Shu Chang ◽  
Zul Ilham ◽  
Adi Ainurzaman Jamaludin ◽  
...  
Keyword(s):  
High Performance ◽  
Three Dimensional ◽  
Biofuel Cell ◽  
Enzymatic Biofuel Cell

scholarly journals Research on Flexible Thin-Disk Glucose Biofuel Cells Based on Single-Walled Carbon Nanotube Electrodes

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Yingying Li ◽  
Wei Xiong ◽  
Cheng Zhang ◽  
Xing Yang
Keyword(s):  
Proton Exchange Membrane ◽  
Direct Electron Transfer ◽  
Economic Benefits ◽  
Open Circuit ◽  
Proton Exchange ◽  
Biofuel Cell ◽  
Promising Alternative ◽  
Practical Applications ◽  
Single Walled Carbon ◽  
Biological Compatibility

Glucose biofuel cell (GBFC) is a power supply device which has attracted considerable attention because of its green environmental protection and high economic benefits. Fuels like glucose and oxygen are ubiquitous in physiological fluids, allowing the direct harvest of energy from human bodies. Compared with conventional batteries such as Li-Po, GBFC is a more promising alternative to power medical devices without the need to be replaced or refueled. However, the energy conversion efficiency of the existing GBFCs still needs to be further improved for practical applications. In this paper, the performance of the GBFC was studied based on single-walled carbon nanotubes (SWCNTs), which have relatively high conductivity and large specific surface area that could improve the activity of enzymes immobilized on the electrode surface and thus realize the direct electron transfer (DET). After optimization of the catalysts’ amount, the GBFC based on SWCNTs performed well with two Pt layers sprayed on one side of the proton exchange membrane (PEM) and 1.5 mL glucose oxidase (GOx) dropped on the other side, which attained the highest open-circuit potential (OCP) of 0.4 V. After being encapsulated with a flexible porous enclosure made by polydimethylsiloxane (PDMS), the biological compatibility of the completed GBFC has been successfully improved, which provides great potential for powering wearable or implantable devices.

scholarly journals Direct Electron Transfer of Cytochrome c on ZnO Nanoparticles Modified Carbon Paste Electrode

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Negahdary ◽  
Saeed Rezaei-Zarchi ◽  
Neda Rousta ◽  
Soheila Samei Pour
Keyword(s):  
Cytochrome C ◽  
Carbon Paste Electrode ◽  
Zno Nanoparticles ◽  
Direct Electron Transfer ◽  
Direct Electrochemistry ◽  
Phosphate Buffer Solution ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Buffer Solution ◽  
Paste Electrode

The direct electrochemistry of cytochrome c (cyt c) immobilized on a modified carbon paste electrode (CPE) was described. The electrode was modified with ZnO nanoparticles. Direct electrochemistry of cytochrome c in this paste electrode was easily achieved, and a pair of well-defined quasireversible redox peaks of a heme Fe (III)/Fe(II) couple appeared with a formal potential (E0) of −0.303 V (versus SCE) in pH 7.0 phosphate buffer solution (PBS). The fabricated modified bioelectrode showed good electrocatalytic ability for reduction of H2O2. The preparation process of the proposed biosensor was convenient, and the resulting biosensor showed high sensitivity, low detection limit, and good stability.

scholarly journals Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Kantapat Chansaenpak ◽  
Anyanee Kamkaew ◽  
Sireerat Lisnund ◽  
Pannaporn Prachai ◽  
Patipat Ratwirunkit ◽  
...  
Keyword(s):  
Carbon Nanostructures ◽  
Glucose Sensor ◽  
Glucose Dehydrogenase ◽  
Food Samples ◽  
Glucose Biosensor ◽  
Biofuel Cell ◽  
Multi Wall Carbon Nanotubes ◽  
Gas Purge ◽  
External Power Source ◽  
Self Powered

Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1–7.0 mM, which is perfectly suited for common biofluids and industrial food samples.

Formate dehydrogenase catalyzedCO2reduction in a chlorin-e6sensitized photochemical biofuel cell

2015 ◽  
Vol 19 (01-03) ◽  
pp. 459-464 ◽  
Author(s):  
Yutaka Amao ◽  
Naho Shuto
Keyword(s):  
Formic Acid ◽  
Visible Light ◽  
Formate Dehydrogenase ◽  
Short Circuit ◽  
Glucose Dehydrogenase ◽  
Open Circuit ◽  
Glass Electrode ◽  
Biofuel Cell ◽  
Conductive Glass ◽  
Open Circuit Photovoltage

The new visible-light operated CO2-glucose biofuel cell consisting of chlorin-e6immobilized on TiO2thin layer film onto optical transparent conductive glass electrode (OTE) as an anode, formate dehydrogenase (FDH) and viologen with long alkyl chain co-immobilized OTE as a cathode, and the solution containing glucose, glucose dehydrogenase (GDH) and NAD+as a fuel was developed. The short-circuit photocurrent and the open-circuit photovoltage of this cell are 37 μA.cm-2and 390 mV, respectively. The maximum power is estimated to be 57 μW.cm-2. The overall photoenergy conversion efficiency is estimated to be 0.057%. After 2 h irradiation to this cell, 0.65 μmol of formic acid was produced. During irradiation, the photocurrent was constant value of 32 ± 10 μA.cm-2in the cell. Thus, CO2reduces and formic acid produces while generating electricity with visible light irradiation to this biofuel cell.

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