TNF-α triggers osteogenic differentiation of human dental pulp stem cells via the NF-κB signalling pathway

2013 ◽  
Vol 37 (12) ◽  
pp. 1267-1275 ◽  
Author(s):  
Xingmei Feng ◽  
Guijuan Feng ◽  
Jing Xing ◽  
Biyu Shen ◽  
Liren Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Signalling Pathway ◽  
Tnf Α ◽  
Human Dental Pulp

High dose of TNF-α suppressed osteogenic differentiation of human dental pulp stem cells by activating the Wnt/β-catenin signaling

2015 ◽  
Vol 46 (4-5) ◽  
pp. 409-420 ◽  
Author(s):  
Zhenjie Qin ◽  
Zhixiu Fang ◽  
Lei Zhao ◽  
Jing Chen ◽  
Yuanteng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
High Dose ◽  
Tnf Α ◽  
Human Dental Pulp

AGS3 is involved in TNF-α medicated osteogenic differentiation of human dental pulp stem cells

2015 ◽  
Vol 89 (5) ◽  
pp. 128-136 ◽  
Author(s):  
Jing Xing ◽  
Min Lian ◽  
Qijie Shen ◽  
Guijuan Feng ◽  
Dan Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Tnf Α ◽  
Human Dental Pulp

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

MiR-21/STAT3 Signal Is Involved in Odontoblast Differentiation of Human Dental Pulp Stem Cells Mediated by TNF-α

2018 ◽  
Vol 20 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Ke Xu ◽  
Jingwen Xiao ◽  
Ke Zheng ◽  
Xingmei Feng ◽  
Jinlong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Odontoblast Differentiation ◽  
Tnf Α ◽  
Human Dental Pulp

scholarly journals Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem Cells In Vitro

2015 ◽  
Vol 21 (3-4) ◽  
pp. 729-739 ◽  
Author(s):  
Jonas Jensen ◽  
David Christian Evar Kraft ◽  
Helle Lysdahl ◽  
Casper Bindzus Foldager ◽  
Muwan Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals A Novel Hypoxic lnc-RNA, HRL-SC, Promotes Proliferation and Migration of Human Dental Pulp Stem Cells Through PI3K/AKT Signalling Pathway

2021 ◽  
Author(s):  
Junkai Zeng ◽  
Ming Chen ◽  
Yeqing Yang ◽  
Buling Wu
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Differential Expression Analysis ◽  
Dental Pulp Stem Cells ◽  
Signalling Pathway ◽  
Gene Set Enrichment Analysis ◽  
Qrt Pcr ◽  
Human Dental Pulp ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background: Human dental pulp stem cells (hDPSCs) are critical for pulp generation. hDPSCs proliferate faster under hypoxia, but the regulatory mechanism of long noncoding RNAs (lncRNAs) in this process is not fully understood.Methods: Novel lncRNAs were obtained by reanalysis of transcriptome datasets coming from RNA-Seq under hypoxia compared with normoxia, and differential expression analysis of target genes were performed. Bioinformatics analyses including Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Set Enrichment Analysis (GSEA) analysis were used to understand the function of key novel lncRNA. hDPSCs were isolated from dental pulp tissue. EdU test and scratch healing test were used to detect the proliferation and migration of hDPSCs. qRT-PCR was used to detect the RNA level expression changes of selected genes. RNA fluorescence in situ hybridization (FISH), small interfering RNA (siRNA), qRT-PCR and western blot analysis were used to explore the function of key novel lncRNA. Results: We identified 496 novel lncRNAs in hDPSCs under hypoxia, including 45 expressed differentially novel lncRNAs. Of them, we focused on a key novel lncRNA, which we named HRL-SC (hypoxia related lncRNA in stem cells). Functional annotation revealed that HRL-SC was associated with hypoxic conditions and PI3K/AKT signalling pathway. HRL-SC was mainly located in the cytoplasm of hDPSCs and had stably high expression under hypoxia. Knockdown of HRL-SC inhibited proliferation and migration of hDPSCs and expression levels of PI3K/AKT related marker proteins. Furthermore, AKT activator SC79 partially offset the inhibitory effect caused by the knockdown, indicating that HRL-SC promoted hDPSCs through PI3K/AKT signalling pathway.Conclusion: Hypoxia related lncRNA HRL-SC promotes proliferation and migration of hDPSCs through PI3K/AKT signalling pathway and it may provide a better understanding for regenerative application of hDPSCs.

Berberine Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Activating EGFR-MAPK-Runx2 Pathways

2019 ◽  
Vol 26 (3) ◽  
pp. 1677-1685 ◽  
Author(s):  
Bing-Chang Xin ◽  
Qi-Shan Wu ◽  
Song Jin ◽  
Ai-Hua Luo ◽  
De-Gang Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp
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