Visual Detection of Adenosine Triphosphate by Taylor Rising: A Simple Point‐of‐Care Testing Method Based on Rolling Circle Amplification

ChemBioChem ◽  
2021 ◽  
Author(s):  
Yong Pan ◽  
Chen Zhu ◽  
Wen Bin Zeng ◽  
Pei Fu ◽  
Chi Chen ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Visual Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Simple Point ◽  
Point Of Care Testing ◽  
Rolling Circle ◽  
Testing Method

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification (SRCA)

2021 ◽  
Author(s):  
Arockiasamy Arun Prince Milton ◽  
Kasanchi M. Momin ◽  
Govindarajan Bhuvana Priya ◽  
Sandeep Ghatak ◽  
Samir Das ◽  
...  
Keyword(s):  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Detection of microRNA: A Point-of-Care Testing Method Based on a pH-Responsive and Highly Efficient Isothermal Amplification

2017 ◽  
Vol 89 (12) ◽  
pp. 6631-6636 ◽  
Author(s):  
Chang Feng ◽  
Xiaoxia Mao ◽  
Hai Shi ◽  
Bing Bo ◽  
Xiaoxia Chen ◽  
...  
Keyword(s):  
Point Of Care ◽  
Isothermal Amplification ◽  
Ph Responsive ◽  
Point Of Care Testing ◽  
Testing Method ◽  
Highly Efficient

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Visual detection of ultra-trace levels of uranyl ions using magnetic bead-based DNAzyme recognition in combination with rolling circle amplification

2017 ◽  
Vol 184 (11) ◽  
pp. 4259-4267 ◽  
Author(s):  
Xian Cheng ◽  
Xinhui Yu ◽  
Lian Chen ◽  
Hongyan Zhang ◽  
Yongning Wu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Magnetic Bead ◽  
Uranyl Ions ◽  
Rolling Circle ◽  
Ultra Trace

Amplified visual detection of microRNA-378 through a T4 DNA ligase-mediated circular template specific to target and target-triggering rolling circle amplification

2019 ◽  
Vol 11 (15) ◽  
pp. 2082-2088 ◽  
Author(s):  
Zhanmin Liu ◽  
Yanming Wang ◽  
Liping Li ◽  
Junhai Li ◽  
Yuanyuan Yuan
Keyword(s):  
Gastric Cancer ◽  
Early Detection ◽  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Serum Biomarker ◽  
Dna Ligase ◽  
Rolling Circle ◽  
T4 Dna Ligase ◽  
Circular Template

MicroRNA-378 (miRNA-378) is widely regarded as a novel noninvasive serum biomarker for early detection of gastric cancer.

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