Amplified visual detection of microRNA-378 through a T4 DNA ligase-mediated circular template specific to target and target-triggering rolling circle amplification

2019 ◽  
Vol 11 (15) ◽  
pp. 2082-2088 ◽  
Author(s):  
Zhanmin Liu ◽  
Yanming Wang ◽  
Liping Li ◽  
Junhai Li ◽  
Yuanyuan Yuan
Keyword(s):  
Gastric Cancer ◽  
Early Detection ◽  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Serum Biomarker ◽  
Dna Ligase ◽  
Rolling Circle ◽  
T4 Dna Ligase ◽  
Circular Template

MicroRNA-378 (miRNA-378) is widely regarded as a novel noninvasive serum biomarker for early detection of gastric cancer.

A fluorescent biosensor for highly specific and ultrasensitive detection of adenosine triphosphate based on ligation-triggered branched rolling circle amplification

2019 ◽  
Vol 11 (36) ◽  
pp. 4629-4636
Author(s):  
Hongxin Jiang ◽  
Guimei Han ◽  
Yaping Xu ◽  
Junxing Li ◽  
Xiaowei Liu ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Rolling Circle Amplification ◽  
Dna Ligase ◽  
Ultrasensitive Detection ◽  
Selective Detection ◽  
Rolling Circle ◽  
T4 Dna Ligase ◽  
Fluorescent Sensing ◽  
Fluorescent Biosensor

A fluorescent sensing strategy for ultrasensitive and highly selective detection of ATP was presented by taking advantage of the exponential amplification capability of BRCA and the extreme specificity of T4 DNA ligase toward ATP.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification (SRCA)

2021 ◽  
Author(s):  
Arockiasamy Arun Prince Milton ◽  
Kasanchi M. Momin ◽  
Govindarajan Bhuvana Priya ◽  
Sandeep Ghatak ◽  
Samir Das ◽  
...  
Keyword(s):  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Genome-wide microRNA profiles identify miR-378 as a serum biomarker for early detection of gastric cancer

2012 ◽  
Vol 316 (2) ◽  
pp. 196-203 ◽  
Author(s):  
Hanshao Liu ◽  
Lin Zhu ◽  
Bingya Liu ◽  
Liu Yang ◽  
Xianxin Meng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Early Detection ◽  
Serum Biomarker ◽  
Genome Wide

scholarly journals Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 352
Author(s):  
Kuankuan Zhang ◽  
Hua Zhang ◽  
Haorui Cao ◽  
Yu Jiang ◽  
Kang Mao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Environmental Pollutants ◽  
Monitoring Method ◽  
Rolling Circle ◽  
Advantages And Disadvantages ◽  
Contaminant Monitoring ◽  
Aqueous Environments ◽  
Global Concern ◽  
Circular Template

Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring.

Visual detection of ultra-trace levels of uranyl ions using magnetic bead-based DNAzyme recognition in combination with rolling circle amplification

2017 ◽  
Vol 184 (11) ◽  
pp. 4259-4267 ◽  
Author(s):  
Xian Cheng ◽  
Xinhui Yu ◽  
Lian Chen ◽  
Hongyan Zhang ◽  
Yongning Wu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Rolling Circle Amplification ◽  
Magnetic Bead ◽  
Uranyl Ions ◽  
Rolling Circle ◽  
Ultra Trace
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