Recent Chemical Approaches for Site‐Specific Conjugation of Native Antibodies: Technologies toward Next‐Generation Antibody–Drug Conjugates

ChemBioChem ◽  
2019 ◽  
Vol 20 (21) ◽  
pp. 2729-2737 ◽  
Author(s):  
Kei Yamada ◽  
Yuji Ito
Keyword(s):  
Next Generation ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Antibody Drug

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

Abstract 752: CovisoLinK: New bacterial transglutaminase Q-Tag substrate for the development of site specific antibody-drug conjugates

2018 ◽  
Author(s):  
Eva Sivado ◽  
Vincent Thomas ◽  
Meddy El Alaoui ◽  
Anne-Catherine Jallas ◽  
Mike R. Dyson ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Antibody Drug

Abstract 756: Development of next-generation antibody-drug conjugates for resistant HER2-positive tumors

2018 ◽  
Author(s):  
Vincent Lacasse ◽  
Jeffrey V. Leyton ◽  
Simon Beaudoin ◽  
Mark Barok ◽  
Heikki Joensuu
Keyword(s):  
Next Generation ◽  
Her2 Positive ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Antibody Drug

Emerging formats for next-generation antibody drug conjugates

2015 ◽  
Vol 10 (5) ◽  
pp. 463-481 ◽  
Author(s):  
Mahendra P Deonarain ◽  
Gokhan Yahioglu ◽  
Ioanna Stamati ◽  
Jared Marklew
Keyword(s):  
Next Generation ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Antibody Drug
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