Effect of Site-Specific Peptide-Tag Labeling on the Biocatalytic Properties of Thermoalkalophilic Lipase from Geobacillus thermocatenulatus

ChemBioChem ◽  
2018 ◽  
Vol 19 (4) ◽  
pp. 369-378 ◽  
Author(s):  
Oscar Romero ◽  
Blanca de las Rivas ◽  
David Lopez-Tejedor ◽  
Jose M. Palomo
Keyword(s):  
Site Specific ◽  
Geobacillus Thermocatenulatus ◽  
Specific Peptide ◽  
Peptide Tag

Design of a Specific Peptide Tag that Affords Covalent and Site-Specific Enzyme Immobilization Catalyzed by Microbial Transglutaminase

2005 ◽  
Vol 6 (4) ◽  
pp. 2299-2304 ◽  
Author(s):  
Jo Tominaga ◽  
Noriho Kamiya ◽  
Satoshi Doi ◽  
Hirofumi Ichinose ◽  
Tatsuo Maruyama ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Microbial Transglutaminase ◽  
Specific Enzyme ◽  
Site Specific ◽  
Specific Peptide ◽  
Peptide Tag

scholarly journals Use of an Asparaginyl Endopeptidase for Chemo-enzymatic Peptide and Protein Labeling

2020 ◽  
Author(s):  
Simon Tang ◽  
Davide Cardella ◽  
Alexander J. Lander ◽  
Xuefei Li ◽  
Yu-Hsuan Tsai ◽  
...  
Keyword(s):  
Phenylboronic Acid ◽  
Product Formation ◽  
Protein Labeling ◽  
Recognition Sequence ◽  
Reaction Conditions ◽  
Site Specific ◽  
Asparaginyl Endopeptidase ◽  
Coupling System ◽  
Labeling System ◽  
Specific Peptide

Transpeptidases are ideal biocatalysts for site-specific peptide and protein labeling, whereas reactions that target N-terminus cysteine with commercially available reagents have become common practice. However, a versatile approach that allows bioconjugation at the terminus of choice (N or C), while avoiding the use of backbone-modified substrates (<i>e.g.</i> depsipeptide) or large excess of reagent, is highly desirable. Aiming to meet these benchmarks, we have combined the advantages of asparaginyl endopeptidase (AEP) catalysis with a N-terminal cysteine trapping reaction and created a chemo-enzymatic labeling system. In this approach, polypeptide with a Asn-Cys-Leu recognition sequence are ligated with a counterpart possessing an N-terminal Gly-Leu by AEP; the byproduct Cys-Leu is subsequently trapped by a stable and inexpensive scavenger, 2-formyl phenylboronic acid (FPBA), to yield an inert thiazolidine derivative, thereby driving the reaction forward to product formation. By carefully screening the reaction conditions for optimal compatibility and minimal hydrolysis, conversion to the ligated product in the model reaction resulted in excellent yields. The versatility of this AEP ligation/FPBA coupling system was further demonstrated by site-specific labeling the N- or C-termini of various proteins.

Site-Specific Peptide Immobilization Strategies for the Rapid Detection of Kinase Activity on Microarrays

2004 ◽  
pp. 191-204 ◽  
Author(s):  
Mahesh Uttamchandani ◽  
Grace Y. J. Chen ◽  
Marie-Laure Lesaicherre ◽  
Shao Q. Yao
Keyword(s):  
Rapid Detection ◽  
Kinase Activity ◽  
Site Specific ◽  
Specific Peptide

Auxiliary-Mediated Site-Specific Peptide Ubiquitylation

2007 ◽  
Vol 119 (16) ◽  
pp. 2872-2876 ◽  
Author(s):  
Champak Chatterjee ◽  
Robert K. McGinty ◽  
Jean-Philippe Pellois ◽  
Tom W. Muir
Keyword(s):  
Site Specific ◽  
Specific Peptide

Chemical Micropatterning of Polycarbonate for Site-Specific Peptide Immobilization and Biomolecular Interactions

ChemBioChem ◽  
2007 ◽  
Vol 8 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Olivier Carion ◽  
Vianney Souplet ◽  
Christophe Olivier ◽  
Céline Maillet ◽  
Nicolas Medard ◽  
...  
Keyword(s):  
Biomolecular Interactions ◽  
Site Specific ◽  
Specific Peptide
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