scholarly journals Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems

ChemBioChem ◽  
2015 ◽  
Vol 16 (9) ◽  
pp. 1365-1370 ◽  
Author(s):  
Zunyi Yang ◽  
Chris McLendon ◽  
Daniel Hutter ◽  
Kevin M. Bradley ◽  
Shuichi Hoshika ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Isothermal Amplification ◽  
Dna And Rna ◽  
Recognition Systems

scholarly journals Recombinase-Based Isothermal Amplification of Nucleic Acids with Self-Avoiding Molecular Recognition Systems (SAMRS)

ChemBioChem ◽  
2014 ◽  
Vol 15 (15) ◽  
pp. 2268-2274 ◽  
Author(s):  
Nidhi Sharma ◽  
Shuichi Hoshika ◽  
Daniel Hutter ◽  
Kevin M. Bradley ◽  
Steven A. Benner
Keyword(s):  
Molecular Recognition ◽  
Nucleic Acids ◽  
Isothermal Amplification ◽  
Recognition Systems

scholarly journals Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Yinhua Zhang ◽  
Guoping Ren ◽  
Jackson Buss ◽  
Andrew J Barry ◽  
Gregory C Patton ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Simple Method ◽  
Diagnostic Assays ◽  
Loop Mediated Isothermal Amplification ◽  
Dna And Rna ◽  
Versatile Technique ◽  
Primer Sets

Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics.

scholarly journals Re-evaluating the conventional wisdom about binding assays

2020 ◽  
Author(s):  
Brandon D. Wilson ◽  
H. Tom Soh
Keyword(s):  
Molecular Recognition ◽  
Conceptual Understanding ◽  
Single Molecule ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Conventional Wisdom ◽  
Binding Assays ◽  
Digital Measurements ◽  
Assay Design ◽  
Recognition Systems

AbstractAnalytical technologies based on binding assays have evolved substantially since their inception nearly 60 years ago, but our conceptual understanding of molecular recognition has not kept pace. Indeed, contemporary technologies such as single-molecule and digital measurements have challenged, or even rendered obsolete, core aspects of the conventional wisdom related to binding assay design. Here, we explore the fundamental principles underlying molecular recognition systems, which we consider in terms of signals generated through concentration-dependent shifts in equilibrium. We challenge certain orthodoxies related to binding-based detection assays, including the primary importance of a low KD and the extent to which this parameter constrains dynamic range and limit of detection. Lastly, we identify key principles for designing binding assays optimally suited for a given detection application.

scholarly journals Selective carbohydrate–lectin interactions in covalent graphene- and SWCNT-based molecular recognition systems

2013 ◽  
Vol 4 (10) ◽  
pp. 4035 ◽  
Author(s):  
Maria-Eleni Ragoussi ◽  
Santiago Casado ◽  
Renato Ribeiro-Viana ◽  
Gema de la Torre ◽  
Javier Rojo ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Recognition Systems

C–H/O interactions of nucleic bases with a water molecule: a crystallographic and quantum chemical study

CrystEngComm ◽  
2014 ◽  
Vol 16 (43) ◽  
pp. 10089-10096 ◽  
Author(s):  
D. Ž. Veljković ◽  
V. B. Medaković ◽  
J. M. Andrić ◽  
S. D. Zarić
Keyword(s):  
Molecular Recognition ◽  
Water Molecule ◽  
Quantum Chemical ◽  
Quantum Chemical Study ◽  
Chemical Study ◽  
Dna And Rna ◽  
Nucleic Bases

The C–H/O interactions of nucleic bases are substantially stronger than the C–H/O interactions of benzene and pyridine. These results can be very important for molecular recognition of DNA and RNA.

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