Genetic Encoding of Caged Cysteine and Caged Homocysteine in Bacterial and Mammalian Cells

ChemBioChem ◽  
2014 ◽  
Vol 15 (12) ◽  
pp. 1793-1799 ◽  
Author(s):  
Rajendra Uprety ◽  
Ji Luo ◽  
Jihe Liu ◽  
Yuta Naro ◽  
Subhas Samanta ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Genetic Encoding

Photoactivation of Innate Immunity Receptor TLR8 in Live Mammalian Cells by Genetic Encoding of Photocaged Tyrosine

ChemBioChem ◽  
2021 ◽  
Author(s):  
Yi Yang ◽  
Shuchen Luo ◽  
Jian Huang ◽  
Yu Xiao ◽  
Yixuan Fu ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Mammalian Cells ◽  
Genetic Encoding

scholarly journals Functional genetic encoding of sulfotyrosine in mammalian cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xinyuan He ◽  
Yan Chen ◽  
Daisy Guiza Beltran ◽  
Maia Kelly ◽  
Bin Ma ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biochemical Characterization ◽  
Trna Synthetase ◽  
Functional Verification ◽  
Cellular Processes ◽  
Genetic Encoding ◽  
Efficient Expression

Abstract Protein tyrosine O-sulfation (PTS) plays a crucial role in extracellular biomolecular interactions that dictate various cellular processes. It also involves in the development of many human diseases. Regardless of recent progress, our current understanding of PTS is still in its infancy. To promote and facilitate relevant studies, a generally applicable method is needed to enable efficient expression of sulfoproteins with defined sulfation sites in live mammalian cells. Here we report the engineering, in vitro biochemical characterization, structural study, and in vivo functional verification of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells. We further apply this chemical biology tool to cell-based studies on the role of a sulfation site in the activation of chemokine receptor CXCR4 by its ligand. Our work will not only facilitate cellular studies of PTS, but also paves the way for economical production of sulfated proteins as therapeutic agents in mammalian systems.

scholarly journals Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells

2014 ◽  
Vol 136 (6) ◽  
pp. 2240-2243 ◽  
Author(s):  
Duy P. Nguyen ◽  
Mohan Mahesh ◽  
Simon J. Elsässer ◽  
Susan M. Hancock ◽  
Chayasith Uttamapinant ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Tev Protease ◽  
Genetic Encoding

scholarly journals Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells

2021 ◽  
Author(s):  
Chloe M. Jones ◽  
D. Miklos Robkis ◽  
Robert J. Blizzard ◽  
Mika Munari ◽  
Yarra Venkatesh ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Mammalian Cells ◽  
Trna Synthetase ◽  
Fluorescence Lifetime Imaging ◽  
High Quantum Yield ◽  
Lifetime Imaging ◽  
Genetic Encoding ◽  
Long Lifetime ◽  
Fluorescent Amino Acid ◽  
Genetic Incorporation

Acridonylalanine (Acd) is photostable, with a high quantum yield and long fluorescence lifetime in water. An evolved tRNA synthetase (RS) enables genetic incorporation of Acd in mammalian cells and its use in fluorescence lifetime imaging microscopy.

Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

2012 ◽  
Vol 134 (29) ◽  
pp. 11912-11915 ◽  
Author(s):  
Eyal Arbely ◽  
Jessica Torres-Kolbus ◽  
Alexander Deiters ◽  
Jason W. Chin
Keyword(s):  
Tyrosine Phosphorylation ◽  
Mammalian Cells ◽  
Genetic Encoding

scholarly journals Front Cover: Reversible and Tunable Photoswitching of Protein Function through Genetic Encoding of Azobenzene Amino Acids in Mammalian Cells (ChemBioChem 20/2018)

ChemBioChem ◽  
2018 ◽  
Vol 19 (20) ◽  
pp. 2120-2120
Author(s):  
Ji Luo ◽  
Subhas Samanta ◽  
Marino Convertino ◽  
Nikolay V. Dokholyan ◽  
Alexander Deiters
Keyword(s):  
Amino Acids ◽  
Protein Function ◽  
Mammalian Cells ◽  
Front Cover ◽  
Genetic Encoding

scholarly journals Photoactivation of Innate Immunity Receptor TLR8 in Live Mammalian Cells by Genetic Encoding of Photocaged Tyrosine

ChemBioChem ◽  
2021 ◽  
Author(s):  
Yi Yang ◽  
Shuchen Luo ◽  
Jian Huang ◽  
Yu Xiao ◽  
Yixuan Fu ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Mammalian Cells ◽  
Genetic Encoding

scholarly journals Reversible and Tunable Photoswitching of Protein Function through Genetic Encoding of Azobenzene Amino Acids in Mammalian Cells

ChemBioChem ◽  
2018 ◽  
Vol 19 (20) ◽  
pp. 2178-2185 ◽  
Author(s):  
Ji Luo ◽  
Subhas Samanta ◽  
Marino Convertino ◽  
Nikolay V. Dokholyan ◽  
Alexander Deiters
Keyword(s):  
Amino Acids ◽  
Protein Function ◽  
Mammalian Cells ◽  
Genetic Encoding
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