Tissue malondialdehyde and adenosine triphosphatase level after experimental liver ischaemia-reperfusion damage

2001 ◽  
Vol 19 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Nevin Ilhan ◽  
Ihsan Halifeoglu ◽  
H. Ibrahim Ozercan ◽  
Necip Ilhan
Keyword(s):  
Adenosine Triphosphatase ◽  
Ischaemia Reperfusion ◽  
Reperfusion Damage ◽  
Experimental Liver ◽  
Liver Ischaemia

THE EFFECT OF 5-AIQ ON THE LIVER ISCHAEMIA/REPERFUSION INJURY

Shock ◽  
2002 ◽  
Vol 18 (Supplement) ◽  
pp. 23
Author(s):  
B. Sepodes ◽  
M. C. McDonald ◽  
R. Pinto ◽  
R. Maio ◽  
M. Caneira-da-Silva ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Liver Ischaemia

Selective portal clamping to minimize hepatic ischaemia–reperfusion damage and avoid accelerated outgrowth of experimental colorectal liver metastases

2006 ◽  
Vol 93 (8) ◽  
pp. 1015-1022 ◽  
Author(s):  
J. D. W. van der Bilt ◽  
O. Kranenburg ◽  
A. Verheem ◽  
R. van Hillegersberg ◽  
I. H. M. Borel Rinkes
Keyword(s):  
Liver Metastases ◽  
Colorectal Liver Metastases ◽  
Ischaemia Reperfusion ◽  
Reperfusion Damage

scholarly journals The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhihui Jiao ◽  
Yajun Ma ◽  
Qianzhen Zhang ◽  
Yue Wang ◽  
Tao Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Function ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Liver Tissue ◽  
Expression Levels ◽  
Miniature Pigs ◽  
Ischaemia Reperfusion ◽  
Tnf Α ◽  
Liver Ischaemia

Abstract Background Hepatic ischaemia-reperfusion injury (HIRI) is inevitable in complicated liver surgery and is a major factor leading to postoperative complications and liver dysfunction. Studies have shown that the paracrine mechanisms of stem cell may be essential to tissue repair and functional improvement after transplantation. However, the role of the adipose-derived mesenchymal stem cell secretome (ASC-secretome) in liver regeneration in large animals remains to be determined. Methods Twenty-four miniature pigs were subjected to laparoscopic liver ischaemia-reperfusion combined with partial hepatectomy and divided into the following four groups: the saline group, the DMEM group, the ASC group and the ASC-secretome group. Serum and liver tissue samples were collected before the operation and at 1, 3 and 7 days after the operation, and changes in tissue pathology, serum inflammation, liver function, angiogenesis-related factors and liver tissue regeneration-related genes and proteins were evaluated. Results Detailed histological analysis showed that ASCs and the ASC-secretome changed pathological damage to liver tissue after liver ischaemia-reperfusion combined with partial hepatectomy (1 and 3 days: p < 0.01). Compared with the saline and DMEM control groups, the ASC-secretome group had significantly reduced expression levels of ALP (1 and 3 days: p < 0.05), ALT (1 day: p < 0.01; 3 days: p < 0.05) and AST (1 and 3 days: p < 0.01), which promoted the recovery of liver function. Moreover, detection of the expression levels of TNF-α and IL-1β (1 day: p < 0.01; 3 days: p < 0.05), IL-6 (1 and 3 days: p < 0.05) and IL-10 (1 and 3 days: p < 0.01) in serum confirmed that the ASC-secretome had obvious anti-inflammatory effects. In addition, the ASC-secretome increased the expression levels of ANG-1 (3 days: p < 0.01), ANG-2 (3 and 7 days: p < 0.01) and VEGF (1 and 7 days: p < 0.05; 3 days: p < 0.01) and promoted angiogenesis during liver regeneration. Moreover, it promoted the mRNA expression of HGF and Cyclin D1 (1 and 3 days: p < 0.01); increased the levels of p-STAT3 (1 and 3 days: p < 0.01), PCNA and Ki67 (1 and 3 days: p < 0.01; 7 days: p < 0.05); inhibited the negative feedback of SOCS3 (1 and 3 days: p < 0.01); and decreased the mRNA expression of TGF-β (3 days: p < 0.01). The cytokines and growth factors detected in the ASC-secretome included TNF-α, IL-6, IL-1β, ANG-1, ANG-2, VEGF and b-FGF. Conclusion The ASC-secretome alleviates the inflammatory response induced by ischaemia-reperfusion combined with partial hepatectomy in miniature pigs and promotes liver regeneration.

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