Differential effects of unsaturated fatty acids on phospholipid synthesis in human leukemia monocytic U937 cells

1993 ◽  
Vol 11 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Arthur J. Chu ◽  
Camtu T. Nguyen ◽  
John Moore
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Human Leukemia ◽  
U937 Cells ◽  
Phospholipid Synthesis ◽  
Differential Effects

Regulation of ethanolamine and choline phosphatide biosynthesis in isolated rat intestinal villus cells

1980 ◽  
Vol 58 (7) ◽  
pp. 527-533 ◽  
Author(s):  
P. J. A. O'Doherty
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lauric Acid ◽  
Unsaturated Fatty Acids ◽  
Phospholipid Synthesis ◽  
Intestinal Villus ◽  
Maximal Stimulation ◽  
Rate Limiting ◽  
Isolated Rat

The effects of ethanolamine, choline, and different fatty acids on phospholipid synthesis via the CDP-ester pathways were studied in isolated rat intestinal villus cells. The incorporation of [14C]glucose into phosphatidylethanolamine was stimulated severalfold by the addition of ethanolamine and long-chained unsaturated fatty acids, while the addition of lauric acid inhibited the incorporation of radioactivity into phosphatidylethanolamine. At concentrations of ethanolamine higher than 0.2 mM, phosphoethanolamine accumulated, but the concentration of CDP-ethanolamine and the incorporation of radioactivity into phosphatidylethanolamine did not increase further. The incorporation of [14C]glucose into phosphatidylcholine responded in a way similar to that of phosphatidylethanolamine, except that a 10-fold higher concentration of choline was required for maximal stimulation. CCC inhibited the incorporation of choline into phosphatidylcholine. In contrast with hepatocytes, villus cells did not form phosphatidylcholine via phospholipid N-methylation. The data indicate that, in intestinal villus cells, the cytidylyltransferase reactions are rate limiting in the synthesis of phosphatidylethanolamine and probably also of phosphatidylcholine. The availability of diacylglycerol and its fatty acid composition may also significantly affect the rate of phospholipid synthesis.

Differential effects of saturated and unsaturated fatty acids on ovarian cancer growth

2017 ◽  
Vol 145 ◽  
pp. 210
Author(s):  
M.H. Uddin ◽  
T.E. Buekers ◽  
M.A. Elshaikh ◽  
S. Giri ◽  
A.R. Munkarah ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Cancer Growth ◽  
Differential Effects

Biosynthesis of unsaturated fatty acids in the main cell lineages of human leukemia and lymphoma

1995 ◽  
Vol 1257 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Isabel Marzo ◽  
María J. Martínez-Lorenzo ◽  
Alberto Anel ◽  
Paula Desportes ◽  
María A. Alava ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Human Leukemia ◽  
Cell Lineages ◽  
Main Cell

Phospholipids in Amelogenesis and Dentinogenesis

2002 ◽  
Vol 13 (3) ◽  
pp. 276-290 ◽  
Author(s):  
M. Goldberg ◽  
D. Septier
Keyword(s):  
Fatty Acids ◽  
Extracellular Matrix ◽  
Osmium Tetroxide ◽  
Unsaturated Fatty Acids ◽  
Collagen Fibrils ◽  
Junctional Complex ◽  
Membrane Domains ◽  
Phospholipid Synthesis ◽  
Coated Pits ◽  
Dental Tissues

Phospholipids have been identified in enamel and dentin. Before demineralization, a group of phospholipids extracted by lipid solvents was associated with cell membranes and is therefore closely related to cell growth and intracellular regulations. After demineralization, a second group of phospholipids, associated with the extracellular matrix, was extracted; this group is probably linked to the mineralized phase. Using imidazole-osmium tetroxide fixation of rat incisors, we stained cellular unsaturated fatty acids, so that we could visualize the membrane domains, coated pits, and endocytic inclusions. Filipin, a probe for cholesterol, varied in density along the plasma membrane of secretory ameloblasts, and allowed us to visualize membrane remnants inside the forming enamel. With respect to phospholipids located in the extracellular matrix, the malachite-green–glutaraldehyde (MGA) method or iodoplatinate (IP) reaction retains and visualizes enamel and dentin phospholipids. In predentin, aggregates appearing as granules and filaments, or liposome-like structures, were located in the spaces between collagen fibrils. In dentin, organic envelopes coating the crystals, also named “crystal-ghost” structures, outlined groups of collagen fibrils. Histochemical data provided evidence that phospholipids are co-distributed or interact with proteoglycans. Radioautography after IP reaction established that [3H] choline was detected in dentin as early as 30 min after the intravenous injection of the labeled precursor, before any labeling was seen in odontoblasts and predentin. This suggests that blood-serum-labeled phospholipids pass between odontoblasts, cross the distal permeable junctional complex, and diffuse in dentin prior to any cellular uptake and phospholipid synthesis. Pharmacologically and genetically induced pathology also supports the suggestion that phospholipids play an important role in the formation and mineralization of dental tissues.

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