Pyruvate kinase activity and response to allosteric effectors in rat erythrocytes and reticulocytes fractionated by multiple partitioning in aqueous two-phase systems

1987 ◽  
Vol 5 (4) ◽  
pp. 301-307 ◽  
Author(s):  
Montserrat Pinilla ◽  
Paz Rodriguez-Horche ◽  
Jose Luque
Keyword(s):  
Pyruvate Kinase ◽  
Kinase Activity ◽  
Pyruvate Kinase Activity ◽  
Rat Erythrocytes ◽  
Two Phase ◽  
Allosteric Effectors ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Changes in glycolytic enzyme activities in aging erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1991 ◽  
Vol 279 (1) ◽  
pp. 237-243 ◽  
Author(s):  
P Jimeno ◽  
A I Garcia-Perez ◽  
J Luque ◽  
M Pinilla
Keyword(s):  
Pyruvate Kinase ◽  
Current Distribution ◽  
Phosphoglycerate Kinase ◽  
Rat Erythrocytes ◽  
Two Phase ◽  
Phase Systems ◽  
Cell Fractions ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Bisphosphoglycerate Mutase

Human and rat erythrocytes were fractionated by counter-current distribution in charge-sensitive dextran/poly(ethylene glycol) two-phase systems. The specific activities of the key glycolytic enzymes (hexokinase, phosphofructokinase and pyruvate kinase) declined along the distribution profiles, although the relative positions of the activity profiles were reversed in the two species. These enzymes maintained their normal response to specific regulatory effectors in all cell fractions. No variations were observed for phosphoglycerate kinase and bisphosphoglycerate mutase activities. Some correlations between enzyme activities (pyruvate kinase/hexokinase, pyruvate kinase/phosphofructokinase, pyruvate kinase/pyruvate kinase plus phosphoglycerate kinase, pyruvate kinase/bisphosphoglycerate mutase and phosphoglycerate kinase/bisphosphoglycerate mutase ratios) were studied in whole erythrocyte populations as well as in cell fractions. These results strongly support the fractionation of human erythrocytes according to cell age, as occurs with rat erythrocytes.

Red blood cell age, pyruvate kinase activity, and insulin receptors. Evidence that monocytes and RBCs may behave differently

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1017-1022 ◽  
Author(s):  
A. Camagna ◽  
R. De Pirro ◽  
L. Tardella ◽  
L. Rossetti ◽  
R. Lauro ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Red Blood Cell ◽  
Kinase Activity ◽  
Insulin Receptors ◽  
Pyruvate Kinase Activity ◽  
Cell Age

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Red Blood Cell Age, Pyruvate Kinase Activity, and Insulin Receptors: Evidence that Monocytes and RBCs May Behave Differently

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1017-1022 ◽  
Author(s):  
A. Camagna ◽  
R. D. Pirro ◽  
L. Tardella ◽  
L. Rossetti ◽  
R. Lauro ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Red Blood Cell ◽  
Kinase Activity ◽  
Insulin Receptors ◽  
Pyruvate Kinase Activity ◽  
Cell Age

Recovery of value-added products from wastewater using Aqueous Two-Phase Systems – A review

2021 ◽  
Vol 778 ◽  
pp. 146293
Author(s):  
Thivaharan Varadavenkatesan ◽  
Shraddha Pai ◽  
Ramesh Vinayagam ◽  
Arivalagan Pugazhendhi ◽  
Raja Selvaraj
Keyword(s):  
Value Added ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Value Added Products
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