scholarly journals Changes in glycolytic enzyme activities in aging erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1991 ◽  
Vol 279 (1) ◽  
pp. 237-243 ◽  
Author(s):  
P Jimeno ◽  
A I Garcia-Perez ◽  
J Luque ◽  
M Pinilla
Keyword(s):  
Pyruvate Kinase ◽  
Current Distribution ◽  
Phosphoglycerate Kinase ◽  
Rat Erythrocytes ◽  
Two Phase ◽  
Phase Systems ◽  
Cell Fractions ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Bisphosphoglycerate Mutase

Human and rat erythrocytes were fractionated by counter-current distribution in charge-sensitive dextran/poly(ethylene glycol) two-phase systems. The specific activities of the key glycolytic enzymes (hexokinase, phosphofructokinase and pyruvate kinase) declined along the distribution profiles, although the relative positions of the activity profiles were reversed in the two species. These enzymes maintained their normal response to specific regulatory effectors in all cell fractions. No variations were observed for phosphoglycerate kinase and bisphosphoglycerate mutase activities. Some correlations between enzyme activities (pyruvate kinase/hexokinase, pyruvate kinase/phosphofructokinase, pyruvate kinase/pyruvate kinase plus phosphoglycerate kinase, pyruvate kinase/bisphosphoglycerate mutase and phosphoglycerate kinase/bisphosphoglycerate mutase ratios) were studied in whole erythrocyte populations as well as in cell fractions. These results strongly support the fractionation of human erythrocytes according to cell age, as occurs with rat erythrocytes.

Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes

1987 ◽  
Vol 7 (2) ◽  
pp. 113-119 ◽  
Author(s):  
J. Luque ◽  
M. D. Delgado ◽  
P. Rodriguez-Horche ◽  
M. T. Company ◽  
M. Pinilla
Keyword(s):  
Ethylene Glycol ◽  
Pyruvate Kinase ◽  
Significant Variation ◽  
Cell Populations ◽  
Red Cell ◽  
Two Phase ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Bisphosphoglycerate Mutase

An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextrahpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.

Heterogeneity of hypotonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueous polymer two-phase systems

1996 ◽  
Vol 677 (1) ◽  
pp. 45-51 ◽  
Author(s):  
M. Teresa Pe´rez ◽  
F. Javier Alvarez ◽  
Ana-Isabel Garci´a-Pe´rez ◽  
Luisa Lucas ◽  
M. Cristina Tejedor ◽  
...  
Keyword(s):  
Current Distribution ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Transferrin binding capacity as a marker of differentiation and maturation of rat erythroid cells fractionated by counter current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 14 (3) ◽  
pp. 119-130
Author(s):  
P. Sancho ◽  
J. Mendieta ◽  
A. I. García-Pérez ◽  
J. Luque
Keyword(s):  
Bone Marrow ◽  
Binding Capacity ◽  
Marrow Cell ◽  
Erythroid Cells ◽  
Two Phase ◽  
Cell Ratio ◽  
Phase Systems ◽  
Cell Fractions ◽  
Counter Current ◽  
Counter Current Distribution

Rat bone marrow cell populations, containing different proportions of erythroid cells, have been fractionated by counter-current distribution in the non-charge-sensitive dextran/polyethyleneglycol two-phase systems on the basis of hydrophobic cell surface properties. Cell fractions with a low distribution coefficient, which contain non-erythroid cells and early erythoblasts, showed a low transferrin binding capacity and a low haemoglobin/cell ratio whereas cell fractions with a high distribution coefficient, which contain intermediate-late erythroblasts and mature red cells, showed an elevated transferrin binding capacity and the highest haemoglobin/cell ratio. These results support transferrin binding capacity as a good marker parameter for the erythroid bone marrow cell differentiation and maturation processes.

Biochemical characterization of human erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 668 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Montserrat Pinilla ◽  
Jesus de la Fuente ◽  
Ana I. García-Pérez ◽  
Pilar Jimeno ◽  
Pilar Sancho ◽  
...  
Keyword(s):  
Current Distribution ◽  
Biochemical Characterization ◽  
Human Erythrocytes ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution
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