Cyclic nucleotides and haemoglobin concentration in rabbit bone marrow cell suspensions stimulated by purified erythropoietin

1984 ◽  
Vol 2 (2) ◽  
pp. 119-124
Author(s):  
G. Fanò ◽  
G. Menchetti ◽  
T. Secca ◽  
V. Marsili ◽  
G. Della Torre
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cyclic Nucleotides ◽  
Marrow Cell ◽  
Haemoglobin Concentration ◽  
Cell Suspensions ◽  
Rabbit Bone

scholarly journals Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1316-1321 ◽  
Author(s):  
WE Fibbe ◽  
J van Damme ◽  
A Billiau ◽  
PJ Voogt ◽  
N Duinkerken ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Interleukin 1 ◽  
Colony Growth ◽  
Cell Suspensions ◽  
Mononuclear Phagocytes ◽  
Adherent Cells ◽  
Macrophage Colony

Abstract An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

scholarly journals Brief Report: The Di Guglielmo Syndrome: Studies in Hemoglobin Synthesis

Blood ◽  
1967 ◽  
Vol 29 (4) ◽  
pp. 550-553 ◽  
Author(s):  
THOMAS F. NECHELES ◽  
WILLIAM DAMESHEK
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Clinical Course ◽  
Marrow Cell ◽  
Cell Suspensions ◽  
In Vitro Synthesis ◽  
Hemoglobin Synthesis ◽  
Heme Synthesis ◽  
Globin Synthesis

Abstract The in vitro synthesis of heme and globin has been studied in bone marrow cell suspensions obtained from five patients with Di Guglielmo syndrome. In all, a defect of heme synthesis was demonstrated, but globin synthesis was greatly reduced in only two of the five; in these two, the clinical course was a rapid one.

The selective removal of red blood cells from bone marrow cell suspensions

1979 ◽  
Vol 3 (3) ◽  
pp. 131-134 ◽  
Author(s):  
F. Triebel ◽  
B. Rio ◽  
J. Tanzer ◽  
P.Goube de Laforest
Keyword(s):  
Bone Marrow ◽  
Red Blood Cells ◽  
Bone Marrow Cell ◽  
Blood Cells ◽  
Marrow Cell ◽  
Cell Suspensions ◽  
Selective Removal

Effect of Intravenously Injected Bone Marrow Cell Suspensions on Thymic Regeneration in Irradiated C 57 Black Mice

Science ◽  
1953 ◽  
Vol 117 (3051) ◽  
pp. 693-695 ◽  
Author(s):  
M. B. Brown ◽  
H. S. Kaplan ◽  
P. P. Weymouth ◽  
J. Paull
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Cell Suspensions ◽  
Thymic Regeneration

scholarly journals Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1316-1321
Author(s):  
WE Fibbe ◽  
J van Damme ◽  
A Billiau ◽  
PJ Voogt ◽  
N Duinkerken ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Interleukin 1 ◽  
Colony Growth ◽  
Cell Suspensions ◽  
Mononuclear Phagocytes ◽  
Adherent Cells ◽  
Macrophage Colony

An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

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