scholarly journals Incidence of bone formation by whole bone marrow cell suspensions and by its stromal cell cultures injected into kidney parenchyma of Balb/c mice

2014 ◽  
Vol 73 (4) ◽  
pp. 482-485
Author(s):  
K. Włodarski ◽  
A. Wojtowicz ◽  
A. Brodzikowska ◽  
R. Galus
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Stromal Cell ◽  
Marrow Cell ◽  
Cell Suspensions

Functional neutrophils from long-term murine bone marrow cell cultures

1987 ◽  
Vol 100 (1-2) ◽  
pp. 223-233 ◽  
Author(s):  
P.H. Hart ◽  
L.K. Spencer ◽  
P.A. Kenny ◽  
A.F. Lopez ◽  
P.J. McDonald ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Murine Bone Marrow ◽  
Murine Bone Marrow Cell

Regulation of the Development of Plaque-Forming Cells to Bromelain-Treated Syngeneic Mouse Erythrocytes in Bone Marrow Cell Cultures

1983 ◽  
Vol 72 (3) ◽  
pp. 253-259 ◽  
Author(s):  
C. Garzelli ◽  
A. Forlani ◽  
S. Lombardi ◽  
V. Colizzi ◽  
M. Campa ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Syngeneic Mouse

scholarly journals Tissue Tropism of the HPRS-103 Strain of J Subgroup Avian Leukosis Virus and of a Derivative Acutely Transforming Virus

1997 ◽  
Vol 34 (2) ◽  
pp. 127-137 ◽  
Author(s):  
S. S. Arshad ◽  
K. Howes ◽  
G. S. Barron ◽  
L. M. Smith ◽  
P. H. Russell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Specific Antigen ◽  
Avian Leukosis Virus ◽  
Bursa Of Fabricius ◽  
Blood Monocyte ◽  
Tissue Tropism ◽  
Myeloid Leukosis

The tissue tropism was studied for the HPRS-103 strain of avian leukosis virus, which belongs to a new envelope subgroup, designated J. Studies were conducted in blood monocyte and bone marrow cell cultures and in chickens from six lines that had been shown previously to differ in susceptibility to induction by this virus of myeloid leukosis and other tumors. Using an immunohistochemical technique to detect expression of viral group-specific antigen (Gag) in various tissues, we detected no major differences among the six lines of chickens at 3 and 7 weeks of age following infection as embryos. Thus, Gag expression did not correlate with differences in tumor susceptibility. Of the tissues examined, greatest Gag expression was observed in cells specific to the adrenal gland, heart, kidney, and proventriculus and especially in smooth muscle cells and connective tissue. After infection of 1-day-old chicks, greater tissue expression was observed in line 21 chicks, which mostly developed a tolerant viremic infection, than in Brown Leghorn chicks, which developed virus-neutralizing antibodies. An acutely transforming virus, strain 966, derived from HPRS-103-induced myeloid leukosis, showed a tropism similar to HPRS-103. The HPRS-103 strain showed a lower propensity to replicate in the medullary region of the lymphoid follicles of the bursa of Fabricius than did the RAV-1 strain of subgroup A avian leukosis virus. This low bursal tropism may be a factor in why HPRS-103 does not induce lymphoid leukosis. The HPRS-103 and 966 virus replicated in blood monocyte cultures from chickens from the six lines, indicating a tropism for the myelomonocytic cell lineage. In comparison, as previously reported, RAV-1 did not replicate well in the monocyte cultures, whereas RAV-2, a subgroup B avian leukosis virus, did replicate. The tropism of HPRS-103 for monocytes may relate to its ability to cause myeloid leukosis. Monocyte and bone marrow cell cultures from the six lines ranked similarly in differences in susceptibility to transformation by 966 virus and showed evidence that their relative susceptibilities correlated with susceptibility of chickens from these lines to induction of myeloid leukosis by HPRS-103, suggesting common tissue-specific viral and host factors involved in oncogenesis by these two viruses.

Established Bone Marrow Cell Cultures with High Degree of Iron Incorporation

Nature ◽  
1962 ◽  
Vol 196 (4851) ◽  
pp. 281-282 ◽  
Author(s):  
GIANCARLO RABOTTI ◽  
IOULIOS IOSSIFIDES
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Iron Incorporation ◽  
High Degree

scholarly journals Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

2019 ◽  
Vol 6 (01) ◽  
pp. e7-e14 ◽  
Author(s):  
Micena Alves e Silva ◽  
Cinthia Pacheco ◽  
Mila Madeira ◽  
Adriana Saraiva ◽  
Elisângela de Freitas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Ethyl Acetate ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Ethanolic Extract ◽  
Quinic Acid ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 ◽  
Hancornia Speciosa

Abstract Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.

scholarly journals Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1316-1321 ◽  
Author(s):  
WE Fibbe ◽  
J van Damme ◽  
A Billiau ◽  
PJ Voogt ◽  
N Duinkerken ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Interleukin 1 ◽  
Colony Growth ◽  
Cell Suspensions ◽  
Mononuclear Phagocytes ◽  
Adherent Cells ◽  
Macrophage Colony

Abstract An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 CFC reached 72%, the number of day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-1 antiserum. Colony growth was abrogated by depleting the marrow cell suspensions of phagocytic cells prior to IL-1 addition. Conversely, the effect could be reintroduced by addition of marrow-derived adherent cells to bone marrow cell suspensions that had been depleted of both phagocytic and E rosetting T cells. Furthermore, media conditioned by bone marrow-derived adherent cells or by peripheral blood mononuclear phagocytes in the presence but not in the absence of IL-1, stimulated in vitro colony growth of phagocyte-depleted bone marrow cell suspensions. These results indicate that IL-1 induces release of granulocyte-macrophage colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.

scholarly journals Brief Report: The Di Guglielmo Syndrome: Studies in Hemoglobin Synthesis

Blood ◽  
1967 ◽  
Vol 29 (4) ◽  
pp. 550-553 ◽  
Author(s):  
THOMAS F. NECHELES ◽  
WILLIAM DAMESHEK
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Clinical Course ◽  
Marrow Cell ◽  
Cell Suspensions ◽  
In Vitro Synthesis ◽  
Hemoglobin Synthesis ◽  
Heme Synthesis ◽  
Globin Synthesis

Abstract The in vitro synthesis of heme and globin has been studied in bone marrow cell suspensions obtained from five patients with Di Guglielmo syndrome. In all, a defect of heme synthesis was demonstrated, but globin synthesis was greatly reduced in only two of the five; in these two, the clinical course was a rapid one.

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