Caffeic Acid Alkyl Amide Derivatives Ameliorate Oxidative Stress and Modulate ERK1/2 and AKT Signaling Pathways in a Rat Model of Diabetic Retinopathy

2019 ◽  
Vol 16 (12) ◽  
Author(s):  
Mohammad Fathalipour ◽  
Masoomeh Eghtedari ◽  
Fernanda Borges ◽  
Tiago Silva ◽  
Fatemeh Moosavi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Signaling Pathways ◽  
Caffeic Acid ◽  
Rat Model ◽  
Akt Signaling ◽  
Amide Derivatives ◽  
Alkyl Amide

Caffeic Acid Phenethyl Ester (CAPE) Protects PC12 Cells Against Cisplatin-Induced Neurotoxicity by Activating the AMPK/SIRT1, MAPK/Erk, and PI3k/Akt Signaling Pathways

2019 ◽  
Vol 36 (1) ◽  
pp. 175-192 ◽  
Author(s):  
Rafaela Scalco Ferreira ◽  
Neife Aparecida Guinaim dos Santos ◽  
Carolina P. Bernardes ◽  
Flávia Malvestio Sisti ◽  
Lilian Amaral ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Caffeic Acid ◽  
Pc12 Cells ◽  
Caffeic Acid Phenethyl Ester ◽  
Akt Signaling

Benzyl Isothiocyanates modulates inflammation, oxidative stress and apoptosis via Nrf-2/HO-1 and NF-κß signaling pathways, on Indomethacin-Induced Gastric injury in Rats

2021 ◽  
Author(s):  
Shymaa A El badawy ◽  
Hanan A. Ogaly ◽  
Reham M. M. Abd-Elsalam ◽  
Asmaa Azouz
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathways ◽  
Rat Model ◽  
Molecular Mechanisms ◽  
Gastric Injury ◽  
Gastroprotective Activity

The present study investigated the gastroprotective activity of benzyl Isothiocyanates (BITC) on indomethacin-induced gastric injury in a rat model and explicated the possible involved biochemical, cellular and molecular mechanisms. Rat...

scholarly journals Suppressive Effects of Rosmarinic Acid Rich Fraction from Perilla on Oxidative Stress, Inflammation and Metastasis Ability in A549 Cells Exposed to PM via C-Jun, P-65-Nf-Κb and Akt Signaling Pathways

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1090
Author(s):  
Komsak Pintha ◽  
Wittaya Chaiwangyen ◽  
Supachai Yodkeeree ◽  
Maitree Suttajit ◽  
Payungsak Tantipaiboonwong
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathways ◽  
Rosmarinic Acid ◽  
Molecular Mechanisms ◽  
Cancer Metastasis ◽  
A549 Cells ◽  
Akt Signaling ◽  
Seed Meal ◽  
Ros Production ◽  
Perilla Seed

Particulate matter from forest fires (PMFF) is an environmental pollutant causing oxidative stress, inflammation, and cancer cell metastasis due to the presence of polycyclic aromatic hydrocarbons (PAHs). Perilla seed meal contains high levels of polyphenols, including rosmarinic acid (RA). The aim of this study is to determine the anti-oxidative stress, anti-inflammation, and anti-metastasis actions of rosmarinic acid rich fraction (RA-RF) from perilla seed meal and its underlying molecular mechanisms in A549 cells exposed to PMFF. PMFF samples were collected via the air sampler at the University of Phayao, Thailand, and their PAH content were analyzed using GC-MS. Fifteen PAH compounds were detected in PMFF. The PMFF significantly induced intracellular reactive oxygen species (ROS) production, the mRNA expression of pro-inflammatory cytokines, MMP-9 activity, invasion, migration, the overexpression of c-Jun and p-65-NF-κB, and Akt phosphorylation. Additionally, the RA-RF significantly reduced ROS production, IL-6, IL-8, TNF-α, and COX-2. RA-RF could also suppress MMP-9 activity, migration, invasion, and the phosphorylation activity of c-Jun, p-65-NF-κB, and Akt. Our findings revealed that RA-RF has antioxidant, anti-inflammatory, and anti-metastasis properties via c-Jun, p-65-NF-κB, and Akt signaling pathways. RA-RF may be further developed as an inhalation agent for the prevention of lung inflammation and cancer metastasis induced by PM exposure.

scholarly journals Antiepileptic Effects of Protein-Rich Extract from Bombyx batryticatus on Mice and Its Protective Effects against H2O2-Induced Oxidative Damage in PC12 Cells via Regulating PI3K/Akt Signaling Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Meibian Hu ◽  
Yujie Liu ◽  
Liying He ◽  
Xing Yuan ◽  
Wei Peng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Signaling Pathways ◽  
Pc12 Cells ◽  
Caspase 3 ◽  
Akt Signaling ◽  
Maximal Electroshock ◽  
Protective Effects ◽  
Caspase 9 ◽  
Protein Rich

Bombyx batryticatus is a known traditional Chinese medicine (TCM) utilized to treat convulsions, epilepsy, cough, asthma, headaches, and purpura in China for thousands of years. This study is aimed at investigating the antiepileptic effects of protein-rich extracts from Bombyx batryticatus (BBPs) on seizure in mice and exploring the protective effects of BBPs against H2O2-induced oxidative stress in PC12 cells and their underlying mechanisms. Maximal electroshock-induced seizure (MES) and pentylenetetrazole- (PTZ-) induced seizure in mice and the histological analysis were carried out to evaluate the antiepileptic effects of BBPs. The cell viability of PC12 cells stimulated by H2O2 was determined by MTT assay. The apoptosis and ROS levels of H2O2-stimulated PC12 cells were determined by flow cytometry analysis. Furthermore, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH), and glutathione (GSH) in PC12 cells were assayed by ELISA and expressions of caspase-3, caspase-9, Bax, Bcl-2, PI3K, Akt, and p-Akt were evaluated by Western blotting and quantitative real-time polymerase chain reaction (RT-qPCR) assays. The results revealed that BBPs exerted significant antiepileptic effects on mice. In addition, BBPs increased the cell viability of H2O2-stimulated PC12 cells and reduced apoptotic cells and ROS levels in H2O2-stimulated PC12 cells. By BBPs treatments, the levels of MDA and LDH were reduced and the levels of SOD and GSH-Px were increased in H2O2-stimulated PC12 cells. Moreover, BBPs upregulated the expressions of PI3K, Akt, p-Akt, and Bcl-2, whereas they downregulated the expressions of caspase-9, caspase-3, and Bax in H2O2-stimulated PC12 cells. These findings suggested that BBPs possessed potential antiepileptic effects on MES and PTZ-induced seizure in mice and protective effects on H2O2-induced oxidative stress in PC12 cells by exerting antioxidative and antiapoptotic effects via PI3K/Akt signaling pathways.

Sign in / Sign up

Export Citation Format

Share Document