scholarly journals Coumestrol mitigates retinal cell inflammation, apoptosis, and oxidative stress in a rat model of diabetic retinopathy via activation of SIRT1

Aging ◽  
2021 ◽  
Author(s):  
Yanchao Xu ◽  
Yusong Zhang ◽  
Hongwei Liang ◽  
Xiaomeng Liu
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Rat Model ◽  
Retinal Cell ◽  
And Oxidative Stress

Fetuin-A exerts a protective effect against experimentally induced intestinal ischemia/reperfusion by suppressing autophagic cell death

2021 ◽  
pp. 153537022199520
Author(s):  
Nanees F El-Malkey ◽  
Amira E Alsemeh ◽  
Wesam MR Ashour ◽  
Nancy H Hassan ◽  
Husam M Edrees
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Protective Role ◽  
Beclin 1 ◽  
Male Rats ◽  
Fetuin A ◽  
Intestinal Ischemia Reperfusion ◽  
And Oxidative Stress

Intestinal tissue is highly susceptible to ischemia/reperfusion injury in many hazardous health conditions. The anti-inflammatory and antioxidant glycoprotein fetuin-A showed efficacy in cerebral ischemic injury; however, its protective role against intestinal ischemia/reperfusion remains elusive. Therefore, this study investigated the protective role of fetuin-A supplementation against intestinal structural changes and dysfunction in a rat model of intestinal ischemia/reperfusion. We equally divided 72 male rats into control, sham, ischemia/reperfusion, and fetuin-A-pretreated ischemia/reperfusion (100 mg/kg/day fetuin-A intraperitoneally for three days prior to surgery and a third dose 1 h prior to the experiment) groups. After 2 h of reperfusion, the jejunum was dissected and examined for spontaneous contractility. A jejunal homogenate was used to assess inflammatory and oxidative stress enzymes. Staining of histological sections was carried out with hematoxylin, eosin and Masson’s trichrome stain for evaluation. Immunohistochemistry was performed to detect autophagy proteins beclin-1, LC3, and p62. This study found that fetuin-A significantly improved ischemia/reperfusion-induced mucosal injury by reducing the percentage of areas of collagen deposition, increasing the amplitude of spontaneous contraction, decreasing inflammation and oxidative stress, and upregulating p62 expression, which was accompanied by beclin-1 and LC3 downregulation. Our findings suggest that fetuin-A treatment can prevent ischemia/reperfusion-induced jejunal structural and functional changes by increasing antioxidant activity and regulating autophagy disturbances observed in the ischemia/reperfusion rat model. Furthermore, fetuin-A may provide a protective influence against intestinal ischemia/reperfusion complications.

scholarly journals Decreased glutamine synthetase, increased citrulline–nitric oxide cycle activities, and oxidative stress in different regions of brain in epilepsy rat model

2010 ◽  
Vol 67 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Mummedy Swamy ◽  
Wan Roslina Wan Yusof ◽  
K. N. S. Sirajudeen ◽  
Zulkarnain Mustapha ◽  
Chandran Govindasamy
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Glutamine Synthetase ◽  
Rat Model ◽  
Nitric Oxide Cycle ◽  
And Oxidative Stress

Carvedilol Attenuates Inflammatory Biomarkers and Oxidative Stress in a Rat Model of Ulcerative Colitis

2015 ◽  
Vol 76 (4) ◽  
pp. 204-214 ◽  
Author(s):  
Amal J. Fatani ◽  
Khaled A. Al-Hosaini ◽  
Mohammed M. Ahmed ◽  
Hatem M. Abuohashish ◽  
Mihir Y. Parmar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Rat Model ◽  
Inflammatory Biomarkers ◽  
And Oxidative Stress

scholarly journals Vitamin D3 regulates apoptosis and proliferation in the testis of D-galactose-induced aged rat model

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Malsawmhriatzuala Jeremy ◽  
Guruswami Gurusubramanian ◽  
Vikas Kumar Roy
Keyword(s):  
Oxidative Stress ◽  
Vitamin D3 ◽  
Rat Model ◽  
Proliferation Markers ◽  
Aged Rat ◽  
Rat Testis ◽  
Defense Systems ◽  
Proliferation And Apoptosis ◽  
Antioxidant Defense Systems ◽  
And Oxidative Stress

Abstract The age-associated imbalances between proliferation and apoptosis lead to impaired spermatogenesis and infertility. The age-associated decline in vitamin D3 levels has been reported and suggested the anti-aging potential of vitamin D3. However, the age-associated decline levels of vitamin D3 has not been studied in relation to the testicular activity. Thus, we investigated the effect of vitamin D3 on the expression of testicular proliferation markers, apoptotic markers, antioxidants system and oxidative stress in a D-gal-induced aged rat model. The present study investigated the levels of vitamin D3 and AGE in serum and testes along with the expression of the AGE-receptor (AGER) in the testis. Vitamin D3 treatment significantly increases cell proliferation and decreases apoptosis in a D-gal-induced aged rat testis. Furthermore, vitamin D3 significantly decreases oxidative stress in aged rat testis by improving the antioxidant defense systems. The expression of AGER was down-regulated by vitamin D3 treatment in aged testis. The circulating and intra-testicular AGE was higher in aged groups, however, only circulating vitamin D3 levels decreased in aged groups. The immunolocalization of VDR showed increased immunostaining in the testis by vitamin D3 treatment. Thus, it can be concluded that vitamin D3 delays testicular senescence by regulating proliferation and apoptosis.

scholarly journals Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Yunhua Wu ◽  
Guangbing Wei ◽  
Junhui Yu ◽  
Zilu Chen ◽  
Zhengshui Xu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
High Dose ◽  
Collagen Deposition ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Danhong Injection ◽  
Intestinal Adhesion ◽  
And Oxidative Stress

Background. Among all the common complications that occur after abdominal surgery, intestinal adhesion is perhaps the most unpleasant one. However, current methods to treat and prevent intestinal adhesion are limited; thus, exploring new methods to prevent and treat intestinal adhesion is greatly needed. In this study, we demonstrated that Danhong injection (DHI) may be used as a promising method to prevent and treat intra-abdominal adhesion in a rat model. Materials and Methods. Forty-eight rats were randomly divided into six groups. Except for the sham-operated group, all rats underwent cecal abrasion to establish an adhesion model. After the operation, the rats in the DHI-treated groups received different doses of DHI via the tail vein daily, while the other group was treated with the same volume of saline solution. Seven days after the operation, all rats were sacrificed, and the degree of adhesion was evaluated by Nair’s scoring system. The extent of inflammation in the adhesion tissue was detected by HE staining and the expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β). The collagen deposition was assessed by Sirius red staining and α-SMA, MMP9, t-PA, and PAI-1 levels. Oxidative stress was indicated by the level of reactive oxygen species (ROS) in adhesion tissues and by immunohistochemical labeling of Nrf2. Furthermore, rat primary peritoneal mesothelial cells (RPMCs) were treated with H2O2 and DHI, and NF-κB phosphorylation was detected to illustrate the effect of DHI on oxidative stress. Results. The intra-abdominal adhesion scores were significantly decreased in the groups treated with a high dose of DHI compared with the control groups, and the degree of inflammation, fibrosis, and oxidative stress was also significantly decreased. DHI treatment significantly reduced the levels of TNF-α, TGF-β1, and PAI and increased the expression levels of MMP9, Nrf2, and t-PA in the adhesion tissues. ROS levels and NF-κB phosphorylation were significantly reduced in DHI-treated RPMCs compared with the control RPMCs. Conclusion. DHI alleviates the formation of postoperative intra-abdominal adhesions by inhibiting inflammation, collagen deposition, and oxidative stress in a rat model and may serve as a promising drug to prevent intra-abdominal adhesions.

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