scholarly journals Multicentric occurrence of multiple papillary thyroid carcinomas – HUMARA and BRAF mutation analysis

2015 ◽  
Vol 4 (8) ◽  
pp. 1272-1280 ◽  
Author(s):  
Tadao Nakazawa ◽  
Tetsuo Kondo ◽  
Ippei Tahara ◽  
Kazunari Kasai ◽  
Tomohiro Inoue ◽  
...  
Keyword(s):  
Mutation Analysis ◽  
Braf Mutation ◽  
Papillary Thyroid ◽  
Multicentric Occurrence ◽  
Thyroid Carcinomas

Quality Control and Telemedicine for BRAF V600E Mutations in Papillary Thyroid Carcinomas

2015 ◽  
Vol 4 (2) ◽  
pp. 12-30 ◽  
Author(s):  
Niki Margari ◽  
Abraham Pouliakis ◽  
Aris Spathis ◽  
Emmanouil Mastorakis ◽  
Efthymios Karakostas ◽  
...  
Keyword(s):  
Quality Control ◽  
Braf Mutation ◽  
Image Features ◽  
Classification And Regression Tree ◽  
Braf V600e ◽  
Papillary Thyroid ◽  
Cell Nuclei ◽  
Braf Mutations ◽  
Mutation Status ◽  
Thyroid Carcinomas

The assessment of BRAF V600E mutations is important for prognosis and treatment of Papillary Thyroid Carcinomas (PTC), the standard methods for their identification are molecular biology techniques. In this study, the potential of image morphometry applied to cell nuclei and sequentially the use of a Classification And Regression Tree (CART) is investigated, in order to: identify morphometric features useful to characterize BRAF mutations, and to eventually produce an algorithm identifying BRAF mutation status. The 140 studied cases had histological confirmation and known BRAF mutation status identified via real-time PCR. The analysis revealed that nuclear features contributing to BRAF mutation status identification via the CART model are related mostly to nuclear color. According to the results there is evidence that BRAF V600E mutations can be identified by measurable image features. Therefore, the proposed method is useful for quality control of BRAF V600E mutations on cytological slides, can serve as alternative to PCR method and may be used for remote assessment.

Reduced expression of THRβ in papillary thyroid carcinomas: relationship with BRAF mutation, aggressiveness and miR expression

2015 ◽  
Vol 38 (12) ◽  
pp. 1283-1289 ◽  
Author(s):  
F. Rosignolo ◽  
V. Maggisano ◽  
M. Sponziello ◽  
M. Celano ◽  
C. R. T. Di Gioia ◽  
...  
Keyword(s):  
Braf Mutation ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

BRAF V600E Mutation Analysis in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-time PCR

2012 ◽  
Vol 20 (3) ◽  
pp. 759-766 ◽  
Author(s):  
Dongjun Jeong ◽  
Yujun Jeong ◽  
Ji Hye Park ◽  
Sun Wook Han ◽  
Sung Yong Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Mutation Analysis ◽  
Real Time Pcr ◽  
Peptide Nucleic Acid ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

scholarly journals Mutation Analysis of RAP1 Gene in Papillary Thyroid Carcinomas

2009 ◽  
Vol 56 (1) ◽  
pp. 161-164 ◽  
Author(s):  
Michiko MATSUSE ◽  
Norisato MITSUTAKE ◽  
Tatiana ROGOUNOVITCH ◽  
Vladimir SAENKO ◽  
Yuka NAKAZAWA ◽  
...  
Keyword(s):  
Mutation Analysis ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

scholarly journals Can Ultrasound Be as a Surrogate Marker for Diagnosing a Papillary Thyroid Cancer? Comparison with BRAF Mutation Analysis

2014 ◽  
Vol 55 (4) ◽  
pp. 871 ◽  
Author(s):  
Jae Young Seo ◽  
Eun-Kyung Kim ◽  
Jung Hwan Baek ◽  
Jung Hee Shin ◽  
Kyung Hwa Han ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Mutation Analysis ◽  
Braf Mutation ◽  
Surrogate Marker ◽  
Papillary Thyroid

scholarly journals Role of Ultrasonographic/Clinical Profile, Cytology, and BRAF V600E Mutation Evaluation in Thyroid Nodule Screening for Malignancy: A Prospective Study

2012 ◽  
Vol 97 (7) ◽  
pp. 2354-2361 ◽  
Author(s):  
Martina Rossi ◽  
Mattia Buratto ◽  
Stefania Bruni ◽  
Carlo Filieri ◽  
Federico Tagliati ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Mutation Analysis ◽  
Braf Mutation ◽  
Thyroid Nodules ◽  
Braf V600e Mutation ◽  
Diagnostic Sensitivity ◽  
Braf V600e ◽  
Additional Contribution ◽  
Papillary Thyroid ◽  
Diagnostic Potential

Abstract Context: Ultrasound (US)-guided fine-needle aspiration biopsy (FNAB) is the most reliable nonsurgical test for distinguishing benign from malignant thyroid nodules. However, there is no consensus on which nodules should undergo FNAB. Aims: The aims of this study were to evaluate the utility of US-guided FNAB in the diagnostic assessment of nodules with or without clinical/US features suggestive for malignancy and to investigate the additional contribution of BRAF V600E mutation analysis in the detection of differentiated thyroid cancer. Design and Methods: Thyroid cytoaspirates from 2421 nodules at least 4 mm in diameter were performed in 1856 patients who underwent cytological evaluation and biomolecular analysis. Results: Cytology showed high positive predictive value and specificity for the diagnosis of malignant lesions. BRAF V600E mutation was found in 115 samples, 80 of which were also cytologically diagnosed as papillary thyroid cancer. BRAF mutation analysis significantly enhanced the diagnostic value of cytology, increasing FNAB diagnostic sensitivity for malignant nodules by approximately 28%. Micro PTC (63% of diagnosed papillary thyroid carcinoma) showed a high prevalence of multifocality, extrathyroidal extension, and lymph node metastases, underlining the malignant potential of thyroid microcarcinomas. Each investigated US/clinical characteristic of suspected malignancy correlated with the presence of a thyroid cancer in thyroid nodules with diameter of at least 4 mm. Conclusions: These data indicate that nodules of at least 4 mm may underlie a thyroid cancer independently of US/clinical characteristics of suspected malignancy, suggesting the need to perform FNAB. The diagnostic sensitivity for thyroid cancer is significantly increased by BRAF V600E mutation analysis, indicating that the screening for BRAF mutation in FNAB samples has a relevant diagnostic potential.

BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas

Pathology ◽  
2006 ◽  
Vol 38 (3) ◽  
pp. 201-204 ◽  
Author(s):  
Ju-Han Lee ◽  
Eung Seok Lee ◽  
Young-Sik Kim ◽  
Nam Hee Won ◽  
Yang-Seok Chae
Keyword(s):  
Braf Mutation ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

scholarly journals High-density microarray expression profiling in conventional papillary thyroid carcinomas with versus without a BRAF mutation

BMC Genomics ◽  
2014 ◽  
Vol 15 (Suppl 2) ◽  
pp. P21
Author(s):  
Reem Alotibi ◽  
Alaa Al-Ahmadi ◽  
Manar Ata ◽  
Sajjad Karim ◽  
Etimad Huwait ◽  
...  
Keyword(s):  
Braf Mutation ◽  
Expression Profiling ◽  
High Density ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas ◽  
Microarray Expression Profiling ◽  
Microarray Expression
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