Three-dimensional analysis of alveolar wall destruction in the early stage of pulmonary emphysema

2014 ◽  
Vol 28 (2) ◽  
pp. 227-234
Author(s):  
Yukihiro Kobayashi ◽  
Takeshi Uehara ◽  
Kenji Kawasaki ◽  
Mitsutoshi Sugano ◽  
Takehisa Matsumoto ◽  
...  
Keyword(s):  
Dimensional Analysis ◽  
Pulmonary Emphysema ◽  
Early Stage ◽  
Three Dimensional ◽  
Alveolar Wall

Three-dimensional analysis of morphogenesis induced by mating pheromone alpha factor in Saccharomyces cerevisiae

1989 ◽  
Vol 94 (2) ◽  
pp. 207-216
Author(s):  
M. Baba ◽  
N. Baba ◽  
Y. Ohsumi ◽  
K. Kanaya ◽  
M. Osumi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dimensional Analysis ◽  
Early Stage ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Membrane Vesicles ◽  
Fixation Method ◽  
Thin Sections ◽  
Alpha Factor ◽  
Intracellular Organelles

Ultrastructural analyses of cytoplasmic changes in Saccharomyces cerevisiae X2180-1A (MATa) that had been treated with alpha factor were performed by using the freeze-substitution fixation method. After alpha factor treatment, cells exhibited a pointed projection, which is a unique pattern of oriented cell surface growth. The relationship between projection formation and intracellular organelles was examined using serial thin sections and computer-aided three-dimensional reconstructions. Using these analyses membrane vesicles and other organelles were detected, and studies on their dynamic structural reorganization became feasible. Production of membrane vesicles (average 65 nm in diameter) was induced upon exposure of the cells to alpha factor before projection emergence. The total number of membrane vesicles increased at the early stage and decreased at the late stage of projection formation. Three-dimensional analysis indicated that the vesicles were at first dispersed throughout the cell, then accumulated at the site where the projection formed. Morphological changes and multiplication of the Golgi body were seen during the process of projection formation. Other intracellular organelles (nucleus, vacuole, rough endoplasmic reticulum and mitochondria) were also rearranged, showing a polar organization of the cytoplasm during projection formation.

In situ investigation of the primary recrystallization of alpha titanium in HVEM

1978 ◽  
Vol 36 (1) ◽  
pp. 634-635
Author(s):  
S. Naka ◽  
R. Penelle ◽  
R. Valle
Keyword(s):  
Dimensional Analysis ◽  
Texture Evolution ◽  
Cold Work ◽  
Three Dimensional ◽  
Primary Recrystallization ◽  
Thin Foils ◽  
In Situ Investigation ◽  
Grain Growth Model ◽  
Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

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