High throughput screening setup of a scale‐down device for membrane chromatography‐aggregate removal of monoclonal antibodies

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Dominik Stein ◽  
Volkmar Thom ◽  
Jürgen Hubbuch
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
High Throughput Screening ◽  
Membrane Chromatography ◽  
Scale Down

scholarly journals Developing an effective scale-down model for a suspension adapted HEK293T-derived lentiviral vector stable producer cell line

2021 ◽  
Author(s):  
Hamza Patel ◽  
Peter Archibald ◽  
Cindy Jung ◽  
Blair Roszell ◽  
Farlan Veraitch ◽  
...  
Keyword(s):  
Production Process ◽  
High Throughput ◽  
High Throughput Screening ◽  
Process Model ◽  
Vaccine Development ◽  
Hek293t Cell ◽  
Stirred Tank ◽  
Bioreactor Culture ◽  
High Cell ◽  
Scale Down

Lentiviral vectors (LVV) represent an important tool for vaccine development and other therapeutic modalities. However, inefficiencies in LVV manufacturing processes, such as the inability to achieve high cell densities with HEK293T cell lines in a fed batch process, have resulted in poor upstream yields. Optimisation of cell culture conditions is needed to improve upstream yields, which can be expedited by high-throughput screening (HTP). In this work, we describe the use of the 24 deep square well (24-DSW) microwell platform to develop a scale-down mimic of GSK’s established stable suspension LVV production process model at 2 L bioreactor scale. We found that matched mixing time was an effective basis for scale-translation between the stirred tank reactor (STR) and microwells. The growth kinetics and LVV productivity profile in the microwell were reproducible and comparable to the 2 L bioreactor process model. In both vessels, a 6-fold increase in cell density was achieved at the harvest time point and high cell viability (i.e. > 90 %) was also maintained throughout the entirety of the cultures. The 24-DSW model, therefore, is an effective scale-down model for larger-scale stirred-tank bioreactor culture and provides an important tool for rapid, high-throughput optimization of the LVV production process.

High throughput screening of ultrafiltration and diafiltration processing of monoclonal antibodies via the ambr® crossflow system

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Lara Fernandez‐Cerezo ◽  
Michael K. Wismer ◽  
InKwan Han ◽  
Jennifer M. Pollard
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
High Throughput Screening

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

2010 ◽  
Vol 37 (9) ◽  
pp. 961-971 ◽  
Author(s):  
Gavin C. Barnard ◽  
Angela R. Kull ◽  
Nathan S. Sharkey ◽  
Seemab S. Shaikh ◽  
Alissa M. Rittenhour ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Yeast Cell ◽  
Cell Lines ◽  
High Throughput ◽  
High Throughput Screening ◽  
Selection Of

Improvement and scale-down of a Trichoderma reesei shake flask protocol to microtiter plates enables high-throughput screening

2014 ◽  
Vol 118 (6) ◽  
pp. 702-709 ◽  
Author(s):  
Heiner Giese ◽  
Paulien Kruithof ◽  
Kristina Meier ◽  
Michaela Sieben ◽  
Elena Antonov ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
High Throughput ◽  
High Throughput Screening ◽  
Shake Flask ◽  
Microtiter Plates ◽  
Scale Down
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