High throughput screening of ultrafiltration and diafiltration processing of monoclonal antibodies via the ambr® crossflow system

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Lara Fernandez‐Cerezo ◽  
Michael K. Wismer ◽  
InKwan Han ◽  
Jennifer M. Pollard
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
High Throughput Screening

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

2010 ◽  
Vol 37 (9) ◽  
pp. 961-971 ◽  
Author(s):  
Gavin C. Barnard ◽  
Angela R. Kull ◽  
Nathan S. Sharkey ◽  
Seemab S. Shaikh ◽  
Alissa M. Rittenhour ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Yeast Cell ◽  
Cell Lines ◽  
High Throughput ◽  
High Throughput Screening ◽  
Selection Of

Robo4 is an effective tumor endothelial marker for antibody-drug conjugates based on the rapid isolation of the anti-Robo4 cell-internalizing antibody

Blood ◽  
2013 ◽  
Vol 121 (14) ◽  
pp. 2804-2813 ◽  
Author(s):  
Mai Yoshikawa ◽  
Yohei Mukai ◽  
Yoshiaki Okada ◽  
Yuki Tsumori ◽  
Shin-ichi Tsunoda ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening System ◽  
Therapeutic Application ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Key Points ◽  
Antibody Drug ◽  
Endothelial Marker

Key Points First therapeutic application that targets Robo4 on the tumor blood vasculature High-throughput screening system to isolate cell-internalizing monoclonal antibodies useful to develop effective antibody-drug conjugates

scholarly journals High-Throughput Screening and Analysis of Charge Variants of Monoclonal Antibodies in Multiple Formulations

2017 ◽  
Vol 22 (8) ◽  
pp. 1044-1052
Author(s):  
Larysa Alekseychyk ◽  
Cheng Su ◽  
Gerald W. Becker ◽  
Michael J. Treuheit ◽  
Vladimir I. Razinkov
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
High Throughput Screening ◽  
Time Frame ◽  
Exchange Chromatography ◽  
Rapid Screening ◽  
Formulation Development ◽  
Charge Variants ◽  
The Right ◽  
High Level

Among different biopharmaceutical products, monoclonal antibodies (mAbs) show a high level of complexity, including heterogeneity due to differences in size, hydrophobicity, charge, and so forth. Such heterogeneity can be related to both cell-based production and any of the stages of purification, storage, and delivery that the mAb is subjected to. Choosing the right formulation composition providing both physical and chemical stabilities can be a very challenging process, especially when done in the limited time frame required for a typical drug development cycle. Charge variants, a common type of heterogeneity for mAbs, are easy to detect by ion exchange, specifically cation exchange chromatography (CEX). We have developed and implemented a high-throughput CEX-based approach for the rapid screening and analysis of charge modifications in multiple formulation conditions. In this work, 96 different formulations of antistreptavidin IgG1 and IgG2 molecules were automatically prepared and analyzed after incubation at high temperature. Design of experiment and statistical analysis tools have been utilized to determine the major formulation factors responsible for chemical stability of antibodies. Regression models were constructed to find the optimal formulation conditions. The methodology can be applied to different stages of preformulation and formulation development of mAbs.

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