Alternating flow filtration as an alternative to internal spin filter based perfusion process: Impact on productivity and product quality

2017 ◽  
Vol 33 (4) ◽  
pp. 1010-1014 ◽  
Author(s):  
Belén Bosco ◽  
Cristian Paillet ◽  
Ignacio Amadeo ◽  
Laura Mauro ◽  
Eduardo Orti ◽  
...  
Keyword(s):  
Product Quality ◽  
Spin Filter ◽  
Perfusion Process ◽  
Flow Filtration

scholarly journals Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

2021 ◽  
Author(s):  
Marc D. Hein ◽  
Anshika Chawla ◽  
Maurizio Cattaneo ◽  
Sascha Y. Kupke ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Hollow Fiber ◽  
Influenza A ◽  
Tubular Cell ◽  
Cell Retention ◽  
Virus Particles ◽  
Tangential Flow ◽  
Perfusion Process ◽  
Flow Filtration ◽  
Retention Device

AbstractRespiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RNA segment. Consequentially, DIPs can only propagate in presence of infectious standard viruses (STVs), compensating the missing gene function. Here, they interfere with and suppress the STV replication and might act “universally” against many IAV subtypes. We recently reported a production system for purely clonal DIPs utilizing genetically modified cells. In the present study, we established an automated perfusion process for production of a DIP, called DI244, using an alternating tangential flow filtration (ATF) system for cell retention. Viable cell concentrations and DIP titers more than 10 times higher than for a previously reported batch cultivation were observed. Furthermore, we investigated a novel tubular cell retention device for its potential for continuous virus harvesting into the permeate. Very comparable performances to typically used hollow fiber membranes were found during the cell growth phase. During the virus replication phase, the tubular membrane, in contrast to the hollow fiber membrane, allowed 100% of the produced virus particles to pass through. To our knowledge, this is the first time a continuous virus harvest was shown for a membrane-based perfusion process. Overall, the process established offers interesting possibilities for advanced process integration strategies for next-generation virus particle and virus vector manufacturing.Key points• An automated perfusion process for production of IAV DIPs was established.• DIP titers of 7.40E + 9 plaque forming units per mL were reached.• A novel tubular cell retention device enabled continuous virus harvesting.

Effects of pyruvate on primary metabolism and product quality for a high‐density perfusion process

2022 ◽  
Author(s):  
Stefania Caso ◽  
Mathieu Aeby ◽  
Martin Jordan ◽  
Raphael Guillot ◽  
Jean‐Marc Bielser
Keyword(s):  
Product Quality ◽  
High Density ◽  
Primary Metabolism ◽  
Perfusion Process

scholarly journals Cell culture-based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

2021 ◽  
Author(s):  
Marc Dominique Hein ◽  
Anshika Chawla ◽  
Maurizio Cattaneo ◽  
Sascha Young Kupke ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Hollow Fiber ◽  
Influenza A ◽  
Hollow Fiber Membrane ◽  
Cell Retention ◽  
Tangential Flow Filtration ◽  
Virus Particles ◽  
Tangential Flow ◽  
Perfusion Process ◽  
Flow Filtration

Respiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RNA segment. Consequentially, DIPs can only propagate in presence of infectious standard viruses (STVs), compensating the missing gene function. Here, they interfere with and suppress the STV replication and might act "universally" against many IAV subtypes. We recently reported a production system for purely clonal DIPs utilizing genetically modified cells. In the present study, we established an automated perfusion process for production of a DIP, called DI244, using an alternating tangential flow filtration (ATF) system for cell retention. Viable cell concentrations and DIP titers more than 10-times higher than for a previously reported batch cultivation were observed. Further, we investigated a novel tubular cell retention device for its potential for continuous virus harvesting into the permeate. Very comparable performances to typically used hollow fiber membranes were found during the cell growth phase. During the virus replication phase the tubular membrane, in contrast to the hollow fiber membrane, allowed 100% of the produced virus particles to pass through. To our knowledge, this is the first time a continuous virus harvest was shown for a membrane-based perfusion process. Overall, the process established offers interesting possibilities for advanced process integration strategies for next-generation virus particle and virus vector manufacturing.

Pengaruh Citra Merek, Kualitas Produk Dan Harga Terhadap Kepuasan Konsumen Pada Bisnis Franchise Minuman

2017 ◽  
Vol 24 (2) ◽  
pp. 144-153
Author(s):  
Yunita Fitri Wahyuningtyas
Keyword(s):  
Regression Analysis ◽  
Marketing Strategy ◽  
Product Quality ◽  
Multiple Regression Analysis ◽  
Multiple Regression ◽  
Sampling Method ◽  
Sampling Technique ◽  
Sampling Process ◽  
And Function ◽  
Simultaneous Influence

This research is conducted upon the emergence of many companies producing the same product of the same kind and function. It leads to the urgency of proper and well planned marketing strategy. This research aims to investigate how far the influence of branding, product quality, and price toward consumer’s satisfaction in beverage franchise business. This research utilizes 5 likert scale questionnaire which is tested by using multiple regression analysis to reveal whether or not there is partial and simultaneous influence of branding, product quality, and price toward consumer’s satisfaction in beverage franchise business. Sampling method is accidental sampling technique, in which sample of particular population is taken based on the accessibility and availability of the sample during the sampling process. Sample used is 100 samples among consumers or customers of Mang Endy Milkshake. The result shows that branding, product quality, and price influence consumer’s satisfaction in beverage franchise business.

Pengembangan Butik Muslim yang Terkait dengan Pelayanan Prima dan Kualitas Produk pada Butik Firta Collection

Liquidity ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 97-107
Author(s):  
Esust Setiawati
Keyword(s):  
Strategic Management ◽  
Product Quality ◽  
Qualitative Method ◽  
Swot Analysis ◽  
External Environment ◽  
Marketing Mix ◽  
External Factors ◽  
Customer Trust

This study aims to analyze how strategic management in the Firta Collection Boutique to develop its business, especially in providing services to customers. The method used in this research is a qualitative method that includes Marketing Mix (4P Analysis) and SWOT Analysis, to find out what external factors are opportunities or threats to the Firta Collection Boutique. The results of the study show that the strategies adopted by the Firta Collection Boutique are still inappropriate and need to use other strategies. Changes in the external environment have continued to increase so that it is an opportunity and anticipates threats by utilizing strengths and minimizing weaknesses by innovating and improving approaches and gaining customer trust in product quality, in order to compete in the fashion sector.

Intra-industry and inter-industry trade through the lens of comparative and absolute advantage

2016 ◽  
pp. 112-128
Author(s):  
A. Gnidchenko
Keyword(s):  
International Trade ◽  
Product Quality ◽  
Vital Role ◽  
Increasing Returns ◽  
Traditional View ◽  
Export Diversification ◽  
Industry Trade ◽  
Long Time ◽  
Absolute Advantage

The article surveys the literature that emphasizes the importance of comparative and absolute advantages for intra- and inter-industry trade. Two conclusions follow form the survey. First, unlike the traditional view, intra-industry trade is determined rather by technology than by increasing returns. Second, absolute advantages that have been ignored in international trade models for a long time play a vital role through their linkages with product quality and export diversification. We also discuss a new strand of literature that models international trade with the assumption of non-homothetic preferences.

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