The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures

2016 ◽  
Vol 32 (5) ◽  
pp. 1113-1122 ◽  
Author(s):  
Jin Hyoung Park ◽  
Myung Sin Lim ◽  
Ju Rang Woo ◽  
Jong Won Kim ◽  
Gyun Min Lee
Keyword(s):  
Molecular Weight ◽  
Cell Growth ◽  
Cell Cultures ◽  
Antibody Production ◽  
Dextran Sulfate ◽  
Cho Cell ◽  
Affect Cell Growth

scholarly journals Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mi Gyeom Kim ◽  
Che Lin Kim ◽  
Young Sik Kim ◽  
Ju Woong Jang ◽  
Gyun Min Lee
Keyword(s):  
Cell Surface ◽  
Heparan Sulfate ◽  
Cell Cultures ◽  
Bone Morphogenetic Proteins ◽  
Cho Cells ◽  
Dextran Sulfate ◽  
Fold Increase ◽  
Cho Cell ◽  
Rcho Cell ◽  
Recombinant Human Bone

AbstractCell surface heparan sulfate proteoglycan (HSPG)-mediated endocytosis results in poor yields of recombinant human bone morphogenetic proteins (rhBMPs) from CHO cell cultures. Upon incubation of rhBMP-2 and rhBMP-7 with CHO cells at 37 °C, both rhBMP-2 and rhBMP-7 bound to the cell surface HSPGs in CHO cells, but only rhBMP-2 was actively internalized into CHO cells. Cell surface HSPGs were found to serve as the main receptor for rhBMP-2 internalization. It was also found that the cell surface HSPG-mediated endocytosis of rhBMP-2 occurred through both the clathrin- and caveolin-dependent pathways. Blockage of rhBMP-2 internalization by the addition of structural analogs of HSPGs such as dextran sulfate (DS) and heparin dramatically increased rhBMP-2 production in recombinant CHO (rCHO) cell cultures. Compared to the control cultures, addition of DS (1.0 g/L) and heparin (0.2 g/L) resulted in a 22.0- and 19.0-fold increase in the maximum rhBMP-2 concentration, respectively. In contrast, the production of rhBMP-7, which was not internalized into the rCHO cells, did not dramatically increase upon addition of DS and heparin. Taken together, rhBMPs have a different fate in terms of HSPG-mediated internalization in CHO cells. HSPG-mediated endocytosis of each rhBMP should be understood individually to increase the rhBMP yield in rCHO cell cultures.

scholarly journals Papillomavirus Microbicidal Activities of High-Molecular-Weight Cellulose Sulfate, Dextran Sulfate, and Polystyrene Sulfonate

2001 ◽  
Vol 45 (12) ◽  
pp. 3427-3432 ◽  
Author(s):  
Neil D. Christensen ◽  
Cynthia A. Reed ◽  
Tim D. Culp ◽  
Paul L. Hermonat ◽  
Mary K. Howett ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Cultures ◽  
Dextran Sulfate ◽  
Transmitted Disease ◽  
In Vitro Assays ◽  
Polystyrene Sulfonate ◽  
Cell Binding ◽  
Cellulose Sulfate ◽  
Microbicidal Activity

ABSTRACT The high-molecular-weight sulfated or sulfonated polysaccharides or polymers cellulose sulfate, dextran sulfate, and polystyrene sulfonate were tested for microbicidal activity against bovine papillomavirus type 1 (BPV-1) and human papillomavirus type 11 (HPV-11) and type 40 (HPV-40). In vitro assays included the BPV-1-induced focus-forming assay and transient infection of human A431 cells with HPVs. The compounds were tested for microbicidal activity directly by preincubation with virus prior to addition to cell cultures and indirectly by addition of virus to compound-treated cells and to virus-coated cells to test inactivation of the virus after virus-cell binding. The data indicated that all three compounds showed direct microbicidal activity with 50% effective concentrations between 10 to 100 μg/ml. These concentrations were nontoxic to cell cultures for both assays. When a clone of C127 cells was tested for microbicidal activity, approximately 10-fold-less compound was required to achieve a 50% reduction in BPV-1-induced foci than for the uncloned parental C127 cells. Pretreatment of cells with compound prior to addition of virus also demonstrated strong microbicidal activity with dextran sulfate and polystyrene sulfonate, but cellulose sulfate required several orders of magnitude more compound for virus inactivation. Polystyrene sulfonate prevented subsequent infection of HPV-11 after virus-cell binding, and this inactivation was observed up to 4 h after addition of virus. These data indicate that the polysulfated and polysulfonated compounds may be useful nontoxic microbicidal compounds that are active against a variety of sexually transmitted disease agents including papillomaviruses.

Manipulation of the sodium‐potassium ratio as a lever for controlling cell growth and improving cell specific productivity in perfusion CHO cell cultures

2018 ◽  
Vol 115 (4) ◽  
pp. 921-931 ◽  
Author(s):  
Samantha B. Wang ◽  
Alexandria Lee‐Goldman ◽  
Janani Ravikrishnan ◽  
Lili Zheng ◽  
Henry Lin
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Specific Productivity ◽  
Cho Cell ◽  
Sodium Potassium

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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