Manipulation of the sodium‐potassium ratio as a lever for controlling cell growth and improving cell specific productivity in perfusion CHO cell cultures

2018 ◽  
Vol 115 (4) ◽  
pp. 921-931 ◽  
Author(s):  
Samantha B. Wang ◽  
Alexandria Lee‐Goldman ◽  
Janani Ravikrishnan ◽  
Lili Zheng ◽  
Henry Lin
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Specific Productivity ◽  
Cho Cell ◽  
Sodium Potassium

The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti Rh D IgG

2001 ◽  
pp. 197-199 ◽  
Author(s):  
Maria J. De Jesus ◽  
M. Bourgeois ◽  
G. Baumgartner ◽  
P. Tromba ◽  
Martin Jordan ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Specific Productivity ◽  
Cho Cell ◽  
Influence Of Ph

Lipidomics of CHO Cell Bioprocessing: Relation to Cell Growth and Specific Productivity of a Monoclonal Antibody

2018 ◽  
Vol 13 (10) ◽  
pp. 1700745 ◽  
Author(s):  
Amr S. Ali ◽  
Ravali Raju ◽  
Somak Ray ◽  
Rashmi Kshirsagar ◽  
Alan Gilbert ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Growth ◽  
Specific Productivity ◽  
Cho Cell

The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures

2016 ◽  
Vol 32 (5) ◽  
pp. 1113-1122 ◽  
Author(s):  
Jin Hyoung Park ◽  
Myung Sin Lim ◽  
Ju Rang Woo ◽  
Jong Won Kim ◽  
Gyun Min Lee
Keyword(s):  
Molecular Weight ◽  
Cell Growth ◽  
Cell Cultures ◽  
Antibody Production ◽  
Dextran Sulfate ◽  
Cho Cell ◽  
Affect Cell Growth

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

Monitoring CHO cell cultures: Cell stress and early apoptosis assessment by mass spectrometry

2013 ◽  
Vol 168 (4) ◽  
pp. 452-461 ◽  
Author(s):  
Sebastian Schwamb ◽  
Bogdan Munteanu ◽  
Björn Meyer ◽  
Carsten Hopf ◽  
Mathias Hafner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Cultures ◽  
Cell Stress ◽  
Cho Cell ◽  
Early Apoptosis

A Comparative Transcriptomics Workflow for Analyzing Microarray Data From CHO Cell Cultures

2017 ◽  
Vol 13 (3) ◽  
pp. 1700228 ◽  
Author(s):  
Chun Chen ◽  
Huong Le ◽  
Brian Follstad ◽  
Chetan T. Goudar
Keyword(s):  
Cell Cultures ◽  
Microarray Data ◽  
Comparative Transcriptomics ◽  
Cho Cell

Na+ Is Responsible for Intra-Run Variations of Specific Productivity of CHO Cell Lines Producing Human Anti Rh D IgG in Bioreactors

2001 ◽  
pp. 193-196
Author(s):  
Maria J. De Jesus ◽  
Lisa Hunt ◽  
M. Bourgeois ◽  
L. Baldi ◽  
Martin Jordan ◽  
...  
Keyword(s):  
Cell Lines ◽  
Specific Productivity ◽  
Cho Cell
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