System for measuring oxygen consumption rates of mammalian cells in static culture under hypoxic conditions

2015 ◽  
Vol 32 (1) ◽  
pp. 189-197 ◽  
Author(s):  
Yuki Kagawa ◽  
Hirotaka Miyahara ◽  
Yuri Ota ◽  
Satoshi Tsuneda
Physiology ◽  
2004 ◽  
Vol 19 (3) ◽  
pp. 110-113 ◽  
Author(s):  
Thorsten Burmester ◽  
Thomas Hankeln

Nerve tissues exhibit some of the highest oxygen consumption rates found in the body. Neuroglobin, a heme protein distantly related to hemoglobin, is thought to enhance the supply of oxygen to the neurons, the eye, and some endocrine tissues. Neuroglobin may promote neuronal survival under hypoxic conditions as they occur, for example, in stroke.


2019 ◽  
Vol 4 (2) ◽  
pp. 79
Author(s):  
Aliati Iswantari ◽  
Kurniawan Kurniawan ◽  
Bambang Priadi ◽  
Vitas Atmadi Prakoso ◽  
Anang Hari Kristanto

<strong>Oxygen Consumption of Barred Loach Nemacheilus fasciatus (Valenciennes, 1846) under Different Stocking Densities</strong>. In aquaculture system, fish growth is affected by stocking densities. One way to predict the effect of stocking density on growth is to determine fish metabolic rate through oxygen consumption measurements. In Barred loach Nemacheilus fasciatus (Valenciennes, 1846), the information was scarce on oxygen consumption. This study was to analyze the effect of stocking density on oxygen consumption in Barred loach conducted at Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor in May 2018. Barred loach (total length: 5.79 ± 0.47 cm, weight: 1.32 ± 0.34 g) was observed its oxygen consumption on three different stocking densities (5, 10, and 15 fish/L) by using closed respirometers (volume: 1.4 L) with three replications of each treatment. Measurement of oxygen consumption was carried out under normoxia and hypoxia conditions. In addition, fish behavior and ventilation rate were also observed and recorded according to treatment. The results showed that the highest oxygen consumption of barred loach was found in the stocking density of 5 fish/L (1250.6 ± 128.4 mg O2/kg/h) which was significantly different from the stocking density of 10 fish/L (626.9 ± 46.7 mg O2/kg/h) and 15 fish/L (596.9 ± 48.9 mg O2/kg/h). Meanwhile, oxygen consumption of barred loach under hypoxic conditions decreased significantly compared to normoxic conditions, which was marked by a decrease in their swimming activities. Although the ventilation rate in hypoxic conditions has decreased, the value was not significantly different from those of normoxia condition. Results of this study provide information that an increase in stocking density and hypoxic conditions in barred loach caused a decrease in oxygen consumption rates. In addition, this study showed that the critical oxygen level for barred loach was around 3.1 mg/L


2012 ◽  
Vol 117 (C2) ◽  
pp. n/a-n/a ◽  
Author(s):  
Clare E. Reimers ◽  
H. Tuba Özkan-Haller ◽  
Peter Berg ◽  
Allan Devol ◽  
Kristina McCann-Grosvenor ◽  
...  

1982 ◽  
Vol 148 (1) ◽  
pp. 35-40 ◽  
Author(s):  
D. F. Houlihan ◽  
A. J. Innes ◽  
M. J. Wells ◽  
J. Wells

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Beth N. Orcutt ◽  
C. Geoffrey Wheat ◽  
Olivier Rouxel ◽  
Samuel Hulme ◽  
Katrina J. Edwards ◽  
...  

1958 ◽  
Vol 35 (2) ◽  
pp. 383-395
Author(s):  
R. W. EDWARDS

1. The oxygen consumption rates of 3rd- and 4th-instar larvae of Chironomus riparius have been measured at 10 and 20° C. using a constant-volume respirometer. 2. The oxygen consumption is approximately proportional to the 0.7 power of the dry weight: it is not proportional to the estimated surface area. 3. This relationship between oxygen consumption and dry weight is the same at 10 and at 20° C.. 4. The rate of oxygen consumption at 20° C. is greater than at 10° C. by a factor of 2.6. 5. During growth the percentage of dry matter of 4th-instar larvae increases from 10 to 16 and the specific gravity from 1.030 to 1.043. 6. The change in the dry weight/wet weight ratio during the 4 larval instar supports the theory of heterauxesis. 7. At 20° C., ‘summer’ larvae respire faster than ‘winter’ larvae.


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