Development of surface-engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene-excimer fluorescence

2013 ◽  
Vol 29 (5) ◽  
pp. 1197-1202 ◽  
Author(s):  
Hideyuki Matsuura ◽  
Yosuke Yamamoto ◽  
Misa Muraoka ◽  
Kenji Akaishi ◽  
Yasuhisa Hori ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Phytochelatin Synthase ◽  
Excimer Fluorescence ◽  
Combined Use ◽  
Engineered Yeast

Enhanced arsenic accumulation by engineered yeast cells expressingArabidopsis thaliana phytochelatin synthase

2007 ◽  
Vol 99 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Shailendra Singh ◽  
Wonkyu Lee ◽  
Nancy A. DaSilva ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Yeast Cells ◽  
Phytochelatin Synthase ◽  
Arsenic Accumulation ◽  
Engineered Yeast

Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells

2014 ◽  
Vol 446 ◽  
pp. 37-43 ◽  
Author(s):  
Jun Ishii ◽  
Asami Oda ◽  
Shota Togawa ◽  
Akira Fukao ◽  
Toshinobu Fujiwara ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Receptor Type ◽  
Fluorescence Sensing ◽  
Neurotensin Receptor ◽  
Engineered Yeast

scholarly journals Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain

2021 ◽  
Author(s):  
Kentaro Inokuma ◽  
Yuki Kitada ◽  
Takahiro Bamba ◽  
Yuma Kobayashi ◽  
Takahiro Yukawa ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cells ◽  
Host Strain ◽  
Engineered Yeast

scholarly journals Biosensors for Detection of Polycyclic Aromatic Hydrocarbons

2019 ◽  
Vol 7 (12) ◽  
pp. 2062
Author(s):  
Ergin Yılmaz ◽  
İdris Yazgan
Keyword(s):  
Firm Value ◽  
Polyaromatic Hydrocarbons ◽  
Living Organism ◽  
Yeast Cells ◽  
Historical Perspectives ◽  
Engineered Yeast ◽  
Sensitivity And Selectivity ◽  
Living Organisms ◽  
Soil Water Resources ◽  
Bioanalytical Techniques

The release of organic pollutants in nature with different forms are treat to the environment and living organism. Particularly, monitoring of the presence of polyaromatic hydrocarbons (PAH) are of great interest due to the fact that they accumulate in soil, water-resources and living organisms and can contaminate food as well. Sensitive and selective detection of PAHs are critical because of the fact that strict regulations governed by national and/international organizations may require different firm value for minimum-allowed concentrations for each PAHs in addition to the total allowed PAHs concentrations. Therefore, analytical and bioanalytical techniques based on different principles have been developed to reach optimized sensitivity and selectivity. Among these techniques, mass spectroscopy coupled chromatographic methods including liquid chromatography (LC) and gas chromatography (GC), and those coupled with spectroscopy are in use to monitor the level and type of the PAH for about 90 years. In addition to these, in last 50 years, biosensors were introduced in detection of PAHs. Particularly, whole-cell based (e.g. engineered yeast cells) and affinity-based (e.g. ELISA) biosensors are currently hot research topics for their simplicity and versatility. In this review, for PAHs detection, historical perspectives, status and outlook along with suggestions are discussed.

scholarly journals Biological upgrading of 3,6-anhydro-l-galactose from agarose to a new platform chemical

2020 ◽  
Vol 22 (5) ◽  
pp. 1776-1785 ◽  
Author(s):  
Dong Hyun Kim ◽  
Jing-Jing Liu ◽  
Jae Won Lee ◽  
Jeffrey G. Pelton ◽  
Eun Ju Yun ◽  
...  
Keyword(s):  
Yeast Cells ◽  
The Novel ◽  
Platform Chemical ◽  
Macroalgal Biomass ◽  
Engineered Yeast

This study demonstrated the novel biological upgrading (using engineered yeast cells) of 3,6-anhydro-l-galactose, the main but untapped sugar of red macroalgal biomass, to 3,6-anhydro-l-galactitol that can be converted to various valuable chemicals including isosorbide.

Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24
Keyword(s):  
Human Insulin ◽  
Manufacturing Process ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Enzymatic Modification ◽  
Engineered Yeast ◽  
Direct Biosynthesis

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin

2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Lipid Production ◽  
Dry Weight ◽  
Genetically Engineered ◽  
Biodiesel Production ◽  
Yeast Cells ◽  
Gene Encoding ◽  
Direct Production ◽  
Inulinase Gene ◽  
Inulinase Activity ◽  
Engineered Yeast

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.

Construction of a Cell Surface Engineered Yeast Aims to Selectively Recover Molybdenum, a Rare Metal

2017 ◽  
Vol 262 ◽  
pp. 421-424 ◽  
Author(s):  
Mei Fang Chien ◽  
Naoya Ikeda ◽  
Kengo Kubota ◽  
Chihiro Inoue
Keyword(s):  
Cell Surface ◽  
Fusion Protein ◽  
Fusion Gene ◽  
Rare Metal ◽  
Yeast Cells ◽  
Functional Domain ◽  
Rare Metals ◽  
Extraction Technology ◽  
Engineered Yeast ◽  
Immunofluorescence Labeling

The depletion of rare metals is an issue of major concern since rare metals are limited in the abundance but essential for high technology industry. However, the present rare metal recovery technical by chemical methods has high environmental impact, poor selectivity, and is too expensive to be practical. To resolve these problems, this study aimed to create a rare metal recover system using yeast, and molybdenum was selected as the first target. A molybdenum binding protein, ModE, which was derived from Escherichia coli was selected. A fusion gene was generated by linking partial modE with a secretion signal and a domain of α-agglutinin to display the ModE on the surface of yeast cells. The expression of fusion protein on the cell surface was detected by immunofluorescence labeling. As for the recovery experiment, the engineered yeast cells were incubated in 10 mM of sodium molybdate solution for 2 h, and the recovery of molybdenum ion was measured by ICP-AES. The results of fluorescence micrographs showed that the designed fusion protein was successfully expressed on yeast cell surface. According to the results of ICP-AES, the cell surface engineered yeast adsorbed molybdenum and the cells after 72~84 h incubation gave the best adsorption. Besides, the results suggested that the optimization of each functional domain in the fusion protein was important. The selectivity and the lower limit of recoverable concentration are under investigation, while this study provides a preliminary result of bio-extraction technology using cell surface engineered yeast.

Sign in / Sign up

Export Citation Format

Share Document