scholarly journals Biological upgrading of 3,6-anhydro-l-galactose from agarose to a new platform chemical

2020 ◽  
Vol 22 (5) ◽  
pp. 1776-1785 ◽  
Author(s):  
Dong Hyun Kim ◽  
Jing-Jing Liu ◽  
Jae Won Lee ◽  
Jeffrey G. Pelton ◽  
Eun Ju Yun ◽  
...  
Keyword(s):  
Yeast Cells ◽  
The Novel ◽  
Platform Chemical ◽  
Macroalgal Biomass ◽  
Engineered Yeast

This study demonstrated the novel biological upgrading (using engineered yeast cells) of 3,6-anhydro-l-galactose, the main but untapped sugar of red macroalgal biomass, to 3,6-anhydro-l-galactitol that can be converted to various valuable chemicals including isosorbide.

Enhanced arsenic accumulation by engineered yeast cells expressingArabidopsis thaliana phytochelatin synthase

2007 ◽  
Vol 99 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Shailendra Singh ◽  
Wonkyu Lee ◽  
Nancy A. DaSilva ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Yeast Cells ◽  
Phytochelatin Synthase ◽  
Arsenic Accumulation ◽  
Engineered Yeast

Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells

2014 ◽  
Vol 446 ◽  
pp. 37-43 ◽  
Author(s):  
Jun Ishii ◽  
Asami Oda ◽  
Shota Togawa ◽  
Akira Fukao ◽  
Toshinobu Fujiwara ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Receptor Type ◽  
Fluorescence Sensing ◽  
Neurotensin Receptor ◽  
Engineered Yeast

scholarly journals Conservation of the Biocatalytic Activity of Whole Yeast Cells by Supported Sol – Gel Entrapment for Efficient Acyloin Condensation

2019 ◽  
Vol 64 (2) ◽  
pp. 153-161 ◽  
Author(s):  
László Nagy-Győr ◽  
Emese Farkas ◽  
Mihai Lăcătuș ◽  
Gergő Tóth ◽  
Dániel Incze ◽  
...  
Keyword(s):  
Sol Gel ◽  
Pyruvate Decarboxylase ◽  
Yeast Cells ◽  
The Novel ◽  
Silica Microspheres ◽  
Promising Alternative ◽  
Hollow Silica ◽  
Gel Entrapment ◽  
Whole Cells ◽  
2Nd Generation

In this study, an efficient and generally applicable 2nd generation sol – gel entrapment method was developed for immobilization of yeastcells. Cells of Lodderomyces elongisporus, Candida norvegica, Debaryomyces fabryi, Pichia carsonii strains in admixture with hollow silica microspheres support were immobilized in sol – gel matrix obtained from polycondensation of tetraethoxysilane. As biocatalysts in theselective acyloin condensation of benzaldehyde catalyzed by pyruvate decarboxylase of the yeast, the novel immobilized whole-cell preparations were compared to other states of the cells such as freshly harvested wet cell paste, lyophilized cells and sol – gel entrapped preparations without hollow silica microspheres support. Reusability and storability studies designated this novel 2nd generation sol – gel method as a promising alternative for solid formulation of whole-cells bypassing expensive and difficult downstream steps while providing easy-to-handle and stable biocatalysts with long-term preservation of the biocatalytic activity.

scholarly journals Intervention of Bro1 in pH-Responsive Rim20 Localization in Saccharomyces cerevisiae

2010 ◽  
Vol 9 (4) ◽  
pp. 532-538 ◽  
Author(s):  
Jacob H. Boysen ◽  
Shoba Subramanian ◽  
Aaron P. Mitchell
Keyword(s):  
Target Genes ◽  
Fluorescent Protein ◽  
Growth Conditions ◽  
Yeast Cells ◽  
Endosomal Trafficking ◽  
The Novel ◽  
Pathway Activation ◽  
Alkaline Conditions ◽  
Acidic Conditions ◽  
Green Fluorescent

ABSTRACT Yeast cells contain two Bro1 domain proteins: Bro1, which is required for endosomal trafficking, and Rim20, which is required for the response to the external pH via the Rim101 pathway. Rim20 associates with endosomal structures under alkaline growth conditions, when it promotes activation of Rim101 through proteolytic cleavage. We report here that the pH-dependent localization of Rim20 is contingent on the amount of Bro1 in the cell. Cells that lack Bro1 have increased endosomal Rim20-green fluorescent protein (GFP) under acidic conditions; cells that overexpress Bro1 have reduced endosomal Rim20-GFP under acidic or alkaline conditions. The novel endosomal association of Rim20-GFP in the absence of Bro1 requires ESCRT components including Vps27 but not specific Rim101 pathway components such as Dfg16. Vps27 influences the localization of Bro1 but is not required for RIM101 pathway activation in wild-type cells, thus suggesting that Rim20 enters the Bro1 localization pathway when a vacancy exists. Despite altered localization of Rim20, the lack of Bro1 does not bypass the need for signaling protein Dfg16 to activate Rim101, as evidenced by the expression levels of the Rim101 target genes RIM8 and SMP1. Therefore, endosomal association of Rim20 is not sufficient to promote Rim101 activation.

scholarly journals Saccharomyces cerevisiae yeast immobilized on marrow stem sunflower and polyacrylamide hydrogels

2014 ◽  
Vol 12 (8) ◽  
pp. 851-857 ◽  
Author(s):  
Ioan Calinescu ◽  
Petre Chipurici ◽  
Elvira Alexandrescu ◽  
Adrian Trifan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Cells ◽  
Industrial Processes ◽  
The Novel ◽  
Fermentation Performance ◽  
Polyacrylamide Hydrogels ◽  
Batch Systems ◽  
Yeast Immobilization ◽  
Positive Effect ◽  
Modified Polyacrylamide

AbstractBiocatalysts with microorganisms immobilized on solid carriers could provide the solution for development of continuous industrial processes for ethanol obtaining by fermentation of sugars. In this study, modified polyacrylamide hydrogels and marrow stem sunflower are used as supports for Saccharomyces cerevisiae yeast immobilization. The obtained structures are used for fermentation of molasses in batch systems. The free yeast cells are used as reference. The modification of polyacrilamide matrix with (2-hydroxyethyl)methacrylate has a positive effect on structure pore uniformity and fermentation performance. The mechanical properties of the obtained biocatalysts are compared. The novel natural matrix has net superior compression strength.

scholarly journals Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain

2021 ◽  
Author(s):  
Kentaro Inokuma ◽  
Yuki Kitada ◽  
Takahiro Bamba ◽  
Yuma Kobayashi ◽  
Takahiro Yukawa ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cells ◽  
Host Strain ◽  
Engineered Yeast

scholarly journals Biosensors for Detection of Polycyclic Aromatic Hydrocarbons

2019 ◽  
Vol 7 (12) ◽  
pp. 2062
Author(s):  
Ergin Yılmaz ◽  
İdris Yazgan
Keyword(s):  
Firm Value ◽  
Polyaromatic Hydrocarbons ◽  
Living Organism ◽  
Yeast Cells ◽  
Historical Perspectives ◽  
Engineered Yeast ◽  
Sensitivity And Selectivity ◽  
Living Organisms ◽  
Soil Water Resources ◽  
Bioanalytical Techniques

The release of organic pollutants in nature with different forms are treat to the environment and living organism. Particularly, monitoring of the presence of polyaromatic hydrocarbons (PAH) are of great interest due to the fact that they accumulate in soil, water-resources and living organisms and can contaminate food as well. Sensitive and selective detection of PAHs are critical because of the fact that strict regulations governed by national and/international organizations may require different firm value for minimum-allowed concentrations for each PAHs in addition to the total allowed PAHs concentrations. Therefore, analytical and bioanalytical techniques based on different principles have been developed to reach optimized sensitivity and selectivity. Among these techniques, mass spectroscopy coupled chromatographic methods including liquid chromatography (LC) and gas chromatography (GC), and those coupled with spectroscopy are in use to monitor the level and type of the PAH for about 90 years. In addition to these, in last 50 years, biosensors were introduced in detection of PAHs. Particularly, whole-cell based (e.g. engineered yeast cells) and affinity-based (e.g. ELISA) biosensors are currently hot research topics for their simplicity and versatility. In this review, for PAHs detection, historical perspectives, status and outlook along with suggestions are discussed.

Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24
Keyword(s):  
Human Insulin ◽  
Manufacturing Process ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Enzymatic Modification ◽  
Engineered Yeast ◽  
Direct Biosynthesis

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

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