Estimation of raw material performance in mammalian cell culture using near infrared spectra combined with chemometrics approaches

2012 ◽  
Vol 28 (3) ◽  
pp. 824-832 ◽  
Author(s):  
Hae Woo Lee ◽  
Andrew Christie ◽  
Jun Jay Liu ◽  
Seongkyu Yoon
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Infrared Spectra ◽  
Near Infrared ◽  
Mammalian Cell Culture ◽  
Raw Material ◽  
Near Infrared Spectra

Semisynthetic model calibration for monitoring glucose in mammalian cell culture with in situ near infrared spectroscopy

2013 ◽  
Vol 111 (5) ◽  
pp. 896-903 ◽  
Author(s):  
Michael Milligan ◽  
Nicholas Lewin-Koh ◽  
Daniel Coleman ◽  
Adeyma Arroyo ◽  
Victor Saucedo
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Mammalian Cell ◽  
Near Infrared Spectroscopy ◽  
Model Calibration ◽  
Near Infrared ◽  
Mammalian Cell Culture

Glucose monitoring and adaptive feeding of mammalian cell culture in the presence of strong autofluorescence by near infrared Raman spectroscopy

2018 ◽  
Vol 34 (6) ◽  
pp. 1574-1580 ◽  
Author(s):  
Thomas E. Matthews ◽  
John P. Smelko ◽  
Brandon Berry ◽  
Saly Romero‐Torres ◽  
Dan Hill ◽  
...  
Keyword(s):  
Cell Culture ◽  
Raman Spectroscopy ◽  
Mammalian Cell ◽  
Near Infrared ◽  
Mammalian Cell Culture ◽  
Glucose Monitoring

Rapid Determining Contents of the Rhubarb Anthraquinones Compounds by Support Vector Machines Modeling based on Near Infrared Spectra

2020 ◽  
Vol 16 ◽  
Author(s):  
Linqi Liu ◽  
JInhua Luo ◽  
Chenxi Zhao ◽  
Bingxue Zhang ◽  
Wei Fan ◽  
...  
Keyword(s):  
Infrared Spectra ◽  
Near Infrared ◽  
Mean Squared Error ◽  
Rapid Determination ◽  
Partial Least Square ◽  
Least Square ◽  
Support Vector ◽  
Near Infrared Spectra ◽  
Aloe Emodin ◽  
Relative Differences

BACKGROUND: Measuring medicinal compounds to evaluate their quality and efficacy has been recognized as a useful approach in treatment. Rhubarb anthraquinones compounds (mainly including aloe-emodin, rhein, emodin, chrysophanol and physcion) are its main effective components as purgating drug. In the current Chinese Pharmacopoeia, the total anthraquinones content is designated as its quantitative quality and control index while the content of each compound has not been specified. METHODS: On the basis of forty rhubarb samples, the correlation models between the near infrared spectra and UPLC analysis data were constructed using support vector machine (SVM) and partial least square (PLS) methods according to Kennard and Stone algorithm for dividing the calibration/prediction datasets. Good models mean they have high correlation coefficients (R2) and low root mean squared error of prediction (RMSEP) values. RESULTS: The models constructed by SVM have much better performance than those by PLS methods. The SVM models have high R2 of 0.8951, 0.9738, 0.9849, 0.9779, 0.9411 and 0.9862 that correspond to aloe-emodin, rhein, emodin, chrysophanol, physcion and total anthraquinones contents, respectively. The corresponding RMSEPs are 0.3592, 0.4182, 0.4508, 0.7121, 0.8365 and 1.7910, respectively. 75% of the predicted results have relative differences being lower than 10%. As for rhein and total anthraquinones, all of the predicted results have relative differences being lower than 10%. CONCLUSION: The nonlinear models constructed by SVM showed good performances with predicted values close to the experimental values. This can perform the rapid determination of the main medicinal ingredients in rhubarb medicinal materials.

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Discrimination of transgenic tomatoes based on visible/near-infrared spectra

2007 ◽  
Vol 584 (2) ◽  
pp. 379-384 ◽  
Author(s):  
Lijuan Xie ◽  
Yibin Ying ◽  
Tiejin Ying ◽  
Haiyan Yu ◽  
Xiaping Fu
Keyword(s):  
Infrared Spectra ◽  
Near Infrared ◽  
Near Infrared Spectra ◽  
Transgenic Tomatoes
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