Enhanced electroporation in plant tissues via low frequency pulsed electric fields: Influence of cytoplasmic streaming

2012 ◽  
Vol 28 (2) ◽  
pp. 445-453 ◽  
Author(s):  
Suvaluk Asavasanti ◽  
Pieter Stroeve ◽  
Diane M. Barrett ◽  
Judith A. Jernstedt ◽  
William D. Ristenpart
Keyword(s):  
Electric Fields ◽  
Cytoplasmic Streaming ◽  
Low Frequency ◽  
Pulsed Electric Fields ◽  
Plant Tissues

Permeabilization of Plant Tissues by Monopolar Pulsed Electric Fields: Effect of Frequency

2010 ◽  
Vol 76 (1) ◽  
pp. E98-E111 ◽  
Author(s):  
Suvaluk Asavasanti ◽  
William Ristenpart ◽  
Pieter Stroeve ◽  
Diane M. Barrett
Keyword(s):  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Plant Tissues

scholarly journals The Literature Review of Electricity Effects on Cell Physiology and Related New Electrical Treatment Modalities

2019 ◽  
Author(s):  
Ertunc Simdi ◽  
Kader Ozcan
Keyword(s):  
Literature Review ◽  
Electric Fields ◽  
Cell Biology ◽  
Experimental Studies ◽  
Low Frequency ◽  
Pulsed Electric Fields ◽  
Treatment Modalities ◽  
Cell Physiology ◽  
Extremely Low Frequency ◽  
New Treatment

This paper is a mini literature review about electromagnetic field’s effects on cells, tissues and new treatment modalities. We have reviewed a papers which have been published in high quality journals in the last 5 years as two authors. This review’s aim is to be a resource for experimental studies about electricity’s effects on cell biology and pathophysiology. As a result of this literature review, we found that especially extremely low electric frequency and intermediate frequency fields have very important pathophysiological effects. We have mentioned four important expressions on this subject; electroporation, electrofusion, electrochemotherapy, gene electrotransference. Two different new treatment approaches have been developed by use of these two important electrical waves. First is tumor treating fields and the other is pulsed electric fields. Most studies in literature have been done with extremely low frequency and pulsed electric fields. In terms of diseases, most studies are about glioblastoma multiforme and malign melanoma.Most studies in literature have been done with extremely low frequency and pulsed electric fields. In terms of diseases, most studies are about glioblastoma and melanoma.

scholarly journals Pulsed Electric Fields (PEF) as pre-treatment for freeze-drying of plant tissues

2018 ◽  
Author(s):  
Virginie Boy ◽  
Lubana Al-Sayed ◽  
Emmanuel Madieta ◽  
Emira Mehinagic ◽  
Jean-Louis Lanoisellé
Keyword(s):  
Electric Fields ◽  
Freeze Drying ◽  
Pulsed Electric Fields ◽  
Textural Properties ◽  
Plant Tissues ◽  
Drying Time ◽  
Pre Treatment

The influence of pulsed electric fields (PEF) treatment on freeze-drying for potato and strawberry tissues was investigated. Samples were pre-treated by PEF (  400 V cm-1) for different treatment times. Freeze-drying was carried out at -17°C and 18.4 Pa or 30 Pa for potato and strawberry tissues, respectively. The effects of PEF pre-treatment was compared with intact samples. The drying time was reduced by 35% for potato and 30% for strawberry. The sample rehydration capacity and the electrolytes released during the rehydration were higher for pre-treated samples. Strawberries texture was characterized by the hardness, the cohesiveness and the springiness.Keywords: Pulsed Electric fields; Freeze-drying; Potato; Strawberry; Textural Properties.   

Pulsed Electric Fields Amd the Transmembrane Potential

1984 ◽  
Vol 3 (1) ◽  
pp. 329-346
Author(s):  
E. R. Strope ◽  
E. Findl ◽  
J. C. Conti ◽  
V. Acuff
Keyword(s):  
Electric Fields ◽  
Transmembrane Potential ◽  
Pulsed Electric Fields

Domain dynamics of strontium-barium niobate crystals in low-frequency electric fields

1990 ◽  
Vol 111 (1) ◽  
pp. 299-305 ◽  
Author(s):  
A. I. Bezhanova ◽  
V. I. Silvestrov ◽  
T. A. Zeinalova ◽  
T. R. Volk
Keyword(s):  
Electric Fields ◽  
Low Frequency ◽  
Strontium Barium Niobate ◽  
Strontium Barium ◽  
Domain Dynamics

scholarly journals Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Marie C. Lefevre ◽  
Gerwin Dijk ◽  
Attila Kaszas ◽  
Martin Baca ◽  
David Moreau ◽  
...  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Flexible Electronics ◽  
Soft Tissues ◽  
Multiphoton Microscopy ◽  
Membrane Integrity ◽  
Tumor Model ◽  
Pulsed Electric Fields ◽  
In Ovo ◽  
Cell Membrane Integrity

AbstractGlioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.

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