scholarly journals A biomimetic hyaluronic acid‐silk fibroin nanofiber scaffold promoting regeneration of transected urothelium

2021 ◽  
Author(s):  
Yuqing Niu ◽  
Massimiliano Galluzzi ◽  
Fuming Deng ◽  
Zhang Zhao ◽  
Ming Fu ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Silk Fibroin ◽  
Nanofiber Scaffold

Efficient Regulation of the Behaviors of Silk Fibroin Hydrogel via Enzyme-Catalyzed Coupling of Hyaluronic Acid

Langmuir ◽  
2020 ◽  
Author(s):  
Lin Wang ◽  
Feiyu Wang ◽  
Bo Xu ◽  
Man Zhou ◽  
Yuanyuan Yu ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Silk Fibroin

scholarly journals Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2302
Author(s):  
Qingwen Yu ◽  
Zhiyuan Meng ◽  
Yichao Liu ◽  
Zehao Li ◽  
Xing Sun ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Regeneration ◽  
Water Uptake ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Ph Value ◽  
Chitosan Nanoparticles ◽  
Vitro Assay ◽  
Long Term Treatment

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

2013 ◽  
Vol 721 ◽  
pp. 274-277
Author(s):  
Li Li Ji ◽  
Qiao Ling Li ◽  
Zeng Hu Yang ◽  
Wei Jing Hu ◽  
Kui Hua Zhang
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Silk Fibroin ◽  
Ethanol Vapor ◽  
Random Coil ◽  
Burst Release ◽  
Nanofibrous Scaffolds ◽  
Vitamin E Tpgs ◽  
Polyethylene Glycol 1000 ◽  
Β Sheet

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

Preparation of Silk Fibroin Nanofiber Scaffold and Its Application in Tendon-Bone Healing and Sports Rehabilitation

2021 ◽  
Vol 13 (7) ◽  
pp. 1374-1382
Author(s):  
Xiaocheng Jiang ◽  
Yuxiang Ren ◽  
Xintao Zhang ◽  
Tian You ◽  
Shiyou Ren ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Bone Healing ◽  
Fibroblast Cell ◽  
Mouse Embryonic Fibroblast ◽  
Control Group ◽  
Mass Ratio ◽  
Gene Expressions ◽  
Postoperative Rehabilitation ◽  
Nanofiber Scaffold ◽  
Nanofiber Scaffolds

ABSTRACTIn this work, polylactic acid/polycaprolactone/silk fibroin (PLA/PCL/SF) nanofiber scaffolds with different mass ratios were prepared by electrospinning technology. The morphology and structure of the nanofiber scaffold were characterized with the scanning electron microscope (SEM), and its porosity and adsorption were tested using the Fourier transform infrared spectrometer (FTIR). 24 New Zealand white rabbits were rolled into control group (n = 8, with autologous tendons) and experimental group (n = 16) randomly. The PLA/PCL/SF nanofiber scaffolds were adopted to wrap autologous tendons to establish extra-articular models. Tendon-bone healing was evaluated six weeks after surgery through histological and biomechanical tests, and the related gene expressions in tissue cells were detected. It turned out that mass ratio of PLA/PCL and SF components had a considerable impact on the morphology of the nanofiber scaffold. The surface of nanofiber with a mass ratio of 3:1 was distributed with dense pores. As the content of SF increased, the porosity and adsorption of the nanofiber scaffold gradually decreased. Moreover, the experimental results suggested that the addition of SF improved the hydrophilicity of PLA/PCL/SF scaffold, which was beneficial to the adhesion and proliferation of NIH/3T3 (a mouse embryonic fibroblast cell line established by the National Institutes of Health (NIH)). In addition, histological observation results showed that the width of the tendon-bone interface (TBI) of rabbits in control group was still relatively large at the 6th week after the surgery, with poor healing effect and disordered collagen arrangement. The widths of the TBI of the material group and rehabilitation group were substantially narrower relative to that in control group, and the collagen was arranged regularly. It was suggested that the healing effects between tendon and bone in material group and rehabilitation group were accelerated, and the effect in the rehabilitation group was superior to that of material group, indicating that rehabilitation exercise could organize the negative effects of training in postoperative rehabilitation training and promote the healing between tendons and bones.

Physically crosslinked silk fibroin/hyaluronic acid scaffolds

2020 ◽  
Vol 239 ◽  
pp. 116232 ◽  
Author(s):  
Yupin Guan ◽  
Haining You ◽  
Junyi Cai ◽  
Qiang Zhang ◽  
Shuqin Yan ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Silk Fibroin ◽  
Physically Crosslinked

Silk Fibroin/Hyaluronic Acid Blend Film with Good Water Stability and Cytocompatibility

2013 ◽  
Vol 377 ◽  
pp. 209-214
Author(s):  
Ling Shuang Wang ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Water Resistance ◽  
High Water ◽  
Casting Method ◽  
Blend Ratio ◽  
Blend Films

Stimulating cell proliferation is a challenge in the field of silk fibroin-based biomaterials. In this study, silk fibroin/hyaluronic acid blend films were prepared by a casting method using carbodiimide as a cross-linking agent. Carbodiimide induced silk fibroin to form Silk I crystal structure which was not affected by the presence of hyaluronic acid. The films showed high water resistance. In vitro, the performance of these films was assessed by seeding L929 cells. The results indicated that the silk fibroin/hyaluronic acid blend films with the blend ratio of 80/20 and 60/40 promoted cell proliferation compared with the pure silk fibroin or hyaluronic acid film. These results suggest that silk fibroin/hyaluronic acid blend films are water stable and cytocompatible materials which are expected to be useful in biomedical applications.

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