Simultaneous determination of pool sizes and fractional turnover rates, of deoxycholic acid, cholic acid and chenodeoxycholic acid in man by isotope dilution with2H and13C labels and serum sampling

1987 ◽  
Vol 14 (11) ◽  
pp. 609-611 ◽  
Author(s):  
F. Stellaard ◽  
M. Sackmann ◽  
F. Berr ◽  
G. Paumgartner
Keyword(s):  
Simultaneous Determination ◽  
Cholic Acid ◽  
Chenodeoxycholic Acid ◽  
Isotope Dilution ◽  
Deoxycholic Acid ◽  
Turnover Rates ◽  
Pool Sizes ◽  
Fractional Turnover

The Formation of Deoxycholic Acid and Chenodeoxycholic Acid in Man

1974 ◽  
Vol 46 (2) ◽  
pp. 183-190
Author(s):  
Kurt Einarsson ◽  
Kjell Hellström
Keyword(s):  
Oral Administration ◽  
Cholic Acid ◽  
Chenodeoxycholic Acid ◽  
Half Life ◽  
Deoxycholic Acid ◽  
Pool Size ◽  
Acid Fraction ◽  
Turnover Rates ◽  
Mean Values ◽  
The Mean

1. The turnover of deoxycholic acid and chenodeoxycholic acid was studied in six normolipaemic patients after oral administration of trace amounts of isotopically labelled compounds. 2. The mean values for half-life, pool size and turnover of deoxycholic acid were 3·0 days, 663 mg and 171 mg/day respectively. The corresponding values recorded for chenodeoxycholic acid were 2·8 days, 781 mg and 207 mg/day. 3. A comparison of the turnover rates of deoxycholic acid and cholic acid in three subjects indicated that 25–61% of the cholic acid was converted into deoxycholic acid. 4. Only trace amounts of radioactivity were recovered in the trihydroxycholanic acid fraction of duodenal bile after the administration of [14C]deoxycholic acid or [3H]chenodeoxycholic acid.

Simultaneous Determination of Alachlor, Metolachlor, Atrazine, and Simazine in Water and Soil by Isotope Dilution Gas Chromatography/Mass Spectrometry

1989 ◽  
Vol 72 (2) ◽  
pp. 349-354 ◽  
Author(s):  
Lee Q Huang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Simultaneous Determination ◽  
Isotope Dilution ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Chromatography Mass Spectrometry

Abstract A multiresidue method was developed for the simultaneous determination of low parts per billion (ppb) concentrations of the herbicides alachlor, metolachlor, atrazine, and simazine in water and soil using isotope dilution gas chromatography/mass spectrometry (GC/MS). Known amounts of 15N,13C-alachlor and 2H5-atrazine were added to each sample as internal standards. The samples were then prepared by a solid phase extraction with no further cleanup. A high resolution GC/low resolution MS system with data acquisition in selected ion monitoring mode was used to quantitate herbicides in the extract. The limit of detection was 0.05 ppb for water and 0.5 ppb for soil. Accuracy greater than 80% and precision better than 4% was demonstrated with spiked samples.

Influence of Deoxycholic Acid on Biliary Lipids in Man

1977 ◽  
Vol 53 (3) ◽  
pp. 249-256 ◽  
Author(s):  
J. Ahlberg ◽  
B. Angelin ◽  
K. Einarsson ◽  
K. Hellström ◽  
B. Leijd
Keyword(s):  
Cholic Acid ◽  
Chenodeoxycholic Acid ◽  
Deoxycholic Acid ◽  
Gall Bladder Disease ◽  
Serum Triglyceride ◽  
Significant Negative Correlation ◽  
Control Subjects ◽  
Cholesterol Saturation ◽  
Biliary Lipid Composition ◽  
Acid Administration

1. The duodenal bile acid composition was analysed in 24 control subjects and 107 patients with various types of hyperlipoproteinaemia. A highly significant negative correlation was observed between the proportions of deoxycholic acid and cholic acid as well as between deoxycholic acid and chenodeoxycholic acid. Patients with gall-bladder disease had an increased proportion of deoxycholic acid in their bile. 2. Eight control subjects were studied before and during the ingestion of 1·9 mmol (0·75 g) of deoxycholic acid daily. In these subjects a rise in the proportion of deoxycholic acid was also accompanied by a fall in the proportion of both cholic acid and chenodeoxycholic acid in duodenal bile. 3. The biliary lipid composition and cholesterol saturation was determined before and during the administration of 1·9 mmol (0·75 g) of chenodeoxycholic acid (n = 12) or deoxycholic acid (n = 8) daily for 3–4 weeks. The cholesterol saturation was decreased during the chenodeoxycholic acid ingestion whereas no change occurred in bile saturation during deoxycholic acid administration. 4. Ingestion of chenodeoxycholic acid lowered serum triglyceride and deoxycholic acid lowered the serum cholesterol.

Sign in / Sign up

Export Citation Format

Share Document