UHPLC‐Q‐TOF‐MS/MS based metabolite profiling of duvelisib and establishment of its metabolism mechanisms

2022 ◽  
Author(s):  
Dipali Sonawane ◽  
Amit Kumar Sahu ◽  
Tarang Jadav ◽  
Pinaki Sengupta
Keyword(s):  
Metabolite Profiling ◽  
Tof Ms

Metabolite profiling of steroidal glycosides in the rhizome of Aspidistra sichuanensis using UPLC/Q-TOF MS E

2017 ◽  
Vol 415 ◽  
pp. 63-84 ◽  
Author(s):  
Jie Peng ◽  
Yang Zhao ◽  
Lu Xu ◽  
Li-ping Kang ◽  
Jiang-ming Cui ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Steroidal Glycosides ◽  
Tof Ms

scholarly journals Analysis of Metabolite Profiling in Human Endothelial Cells after Plasma Jet Treatment

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Yanjie Yang ◽  
Dehui Xu ◽  
Ning Ning ◽  
Yujing Xu
Keyword(s):  
Endothelial Cells ◽  
Plasma Treatment ◽  
Metabolite Profiling ◽  
Atmospheric Plasma ◽  
Human Endothelial Cells ◽  
Metabolic Pathway Analysis ◽  
Orthogonal Projections ◽  
Blood Capillaries ◽  
Latent Structures ◽  
Tof Ms

Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in wound healing, dermatological treatment, hemostasis, and cancer treatment. In most cases, CAP treatment will interact with innumerable blood capillaries. Therefore, it is important and necessary to understand the effects of CAP treatment on endothelial cell metabolism. In this study, the metabolite profiling of plasma treatment on endothelial cells was measured by gas chromatography tandem time-of-flight mass spectrometry (GC-TOF-MS). We found that 695 signals (metabolites) were detected by GC-TOF-MS and then evaluated using orthogonal projections to latent structures discriminant analysis (OPLS-DA). All the differential metabolites were listed, and proline and xanthosine were the two of the most downregulated metabolites by plasma treatment. By comprehensive metabolic pathway analysis with the KEGG pathway, we showed that alanine, aspartate, glutamate, and purine metabolism pathways were the most significantly suppressed after gas plasma treatment in human endothelial cells. Our finding gives an overall picture of the metabolic pathways affected by plasma treatment in endothelial cells.

UPLC-ESI-TOF MS-Based Metabolite Profiling of the Antioxidative Food Supplement Garcinia buchananii

2015 ◽  
Vol 63 (32) ◽  
pp. 7169-7179 ◽  
Author(s):  
Timo D. Stark ◽  
Sofie Lösch ◽  
Junichiro Wakamatsu ◽  
Onesmo B. Balemba ◽  
Oliver Frank ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Food Supplement ◽  
Garcinia Buchananii ◽  
Tof Ms

scholarly journals Metabolite profiling of RCS-4, a novel synthetic cannabinoid designer drug, using human hepatocyte metabolism and TOF-MS

Bioanalysis ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 1471-1485 ◽  
Author(s):  
Adarsh S Gandhi ◽  
Mingshe Zhu ◽  
Shaokun Pang ◽  
Ariane Wohlfarth ◽  
Karl B Scheidweiler ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Human Hepatocyte ◽  
Synthetic Cannabinoid ◽  
Designer Drug ◽  
Hepatocyte Metabolism ◽  
Tof Ms

Metabolite profiling of Zi-Shen pill in rat biological specimens by UPLC-Q-TOF/MS

2015 ◽  
Vol 13 (2) ◽  
pp. 145-160 ◽  
Author(s):  
Xiao-Wei LIU ◽  
Feng ZHANG ◽  
Shou-Hong GAO ◽  
Bo JIANG ◽  
Wan-Sheng CHEN
Keyword(s):  
Metabolite Profiling ◽  
Tof Ms

scholarly journals Metabolite Profiling of Dihydroartemisinin in Blood of Plasmodium-Infected and Healthy Mice Using UPLC-Q-TOF-MSE

2021 ◽  
Vol 11 ◽  
Author(s):  
Yifan Zhao ◽  
Peng Sun ◽  
Yue Ma ◽  
Xiaoqiang Chang ◽  
Xingyu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Metabolic Pathway ◽  
Blood Cells ◽  
Metabolite Profiling ◽  
First Line ◽  
First Time ◽  
Insight Into ◽  
Tof Ms

Dihydroartemisinin (DHA) and its’ derivatives have been employed as the most powerful first-line drugs for malarial treatment for several decades. The metabolism of DHA has not been studied clearly. Previous reports were focused on the pharmacokinetics procedure of DHA in healthy rats. The metabolites of DHA in red blood cells (RBC), especially in the RBC from Plasmodium-infected models, have rarely been studied. The Plasmodium species parasitize inside RBC, and these cells should be the final place where DHA performs its activity. In this study, the profile of DHA metabolites in biosample (blood, plasma, and RBC) of the infected and healthy mice was investigated with UPLC-Q-TOF-MS and UNIFI platform to gain insight into DHA metabolism. Results show that a total of 25 metabolites were successfully identified in infected (30 in healthy) blood, 27 in infected (27 in healthy) plasma, and 15 in infected (22 in healthy) RBC. Results show that hydroxylation, OH-dehydration, and glucuronidation reactions were important in the metabolic pathway in vivo. Significantly, DHA metabolites inside RBC were identified for the first time. 8-Hydroxy (8-OH) DHA, 4α-OH deoxy ART, and 6β-OH deoxy ART were identified in vivo for the first time.

scholarly journals Optimization of Metabolite Extraction Protocols for Untargeted Metabolite Profiling of Mycoparasitic Scytalidium parasiticum using LC-TOF-MS

2018 ◽  
Vol 47 (12) ◽  
pp. 3061-3068 ◽  
Author(s):  
Choon Kiat Lim ◽  
Rafidah Ahmad ◽  
Nurul Fadhilah Marzuki ◽  
Yit Kheng Goh ◽  
Kamalrul Azlan Azizan ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Untargeted Metabolite Profiling ◽  
Metabolite Extraction ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document