scholarly journals Metabolite profiling of RCS-4, a novel synthetic cannabinoid designer drug, using human hepatocyte metabolism and TOF-MS

Bioanalysis ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 1471-1485 ◽  
Author(s):  
Adarsh S Gandhi ◽  
Mingshe Zhu ◽  
Shaokun Pang ◽  
Ariane Wohlfarth ◽  
Karl B Scheidweiler ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Human Hepatocyte ◽  
Synthetic Cannabinoid ◽  
Designer Drug ◽  
Hepatocyte Metabolism ◽  
Tof Ms

scholarly journals Pyrrolidinyl Synthetic Cathinones α-PHP and 4F-α-PVP Metabolite Profiling Using Human Hepatocyte Incubations

2020 ◽  
Vol 22 (1) ◽  
pp. 230
Author(s):  
Jeremy Carlier ◽  
Xingxing Diao ◽  
Raffaele Giorgetti ◽  
Francesco P. Busardò ◽  
Marilyn A. Huestis
Keyword(s):  
Metabolite Profiling ◽  
Human Liver ◽  
Liver Microsomes ◽  
Drug Market ◽  
Metabolite Profile ◽  
Illegal Drug ◽  
Human Hepatocyte ◽  
Synthetic Cathinones ◽  
Metabolic Fate ◽  
Analytical Standards

For more than ten years, new synthetic cathinones (SCs) mimicking the effects of controlled cocaine-like stimulants have flooded the illegal drug market, causing numerous intoxications and fatalities. There are often no data on the pharmacokinetics of these substances when they first emerge onto the market. However, the detection of SC metabolites is often critical in order to prove consumption in clinical and forensic settings. In this research, the metabolite profile of two pyrrolidinyl SCs, α-pyrrolidinohexaphenone (α-PHP) and 4′′-fluoro-α-pyrrolidinovalerophenone (4F-α-PVP), were characterized to identify optimal intake markers. Experiments were conducted using pooled human hepatocyte incubations followed by liquid chromatography–high-resolution tandem mass spectrometry and data-mining software. We suggest α-PHP dihydroxy-pyrrolidinyl, α-PHP hexanol, α-PHP 2′-keto-pyrrolidinyl-hexanol, and α-PHP 2′-keto-pyrrolidinyl as markers of α-PHP use, and 4F-α-PVP dihydroxy-pyrrolidinyl, 4F-α-PVP hexanol, 4F-α-PVP 2′-keto-pyrrolidinyl-hexanol, and 4F-α-PVP 2′-keto-pyrrolidinyl as markers of 4F-α-PVP use. These results represent the first data available on 4F-α-PVP metabolism. The metabolic fate of α-PHP was previously studied using human liver microsomes and urine samples from α-PHP users. We identified an additional major metabolite (α-PHP dihydroxy-pyrrolidinyl) that might be crucial for documenting exposure to α-PHP. Further experiments with suitable analytical standards, which are yet to be synthesized, and authentic specimens should be conducted to confirm these results.

Metabolite profiling of steroidal glycosides in the rhizome of Aspidistra sichuanensis using UPLC/Q-TOF MS E

2017 ◽  
Vol 415 ◽  
pp. 63-84 ◽  
Author(s):  
Jie Peng ◽  
Yang Zhao ◽  
Lu Xu ◽  
Li-ping Kang ◽  
Jiang-ming Cui ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Steroidal Glycosides ◽  
Tof Ms

scholarly journals Analysis of Metabolite Profiling in Human Endothelial Cells after Plasma Jet Treatment

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Yanjie Yang ◽  
Dehui Xu ◽  
Ning Ning ◽  
Yujing Xu
Keyword(s):  
Endothelial Cells ◽  
Plasma Treatment ◽  
Metabolite Profiling ◽  
Atmospheric Plasma ◽  
Human Endothelial Cells ◽  
Metabolic Pathway Analysis ◽  
Orthogonal Projections ◽  
Blood Capillaries ◽  
Latent Structures ◽  
Tof Ms

Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in wound healing, dermatological treatment, hemostasis, and cancer treatment. In most cases, CAP treatment will interact with innumerable blood capillaries. Therefore, it is important and necessary to understand the effects of CAP treatment on endothelial cell metabolism. In this study, the metabolite profiling of plasma treatment on endothelial cells was measured by gas chromatography tandem time-of-flight mass spectrometry (GC-TOF-MS). We found that 695 signals (metabolites) were detected by GC-TOF-MS and then evaluated using orthogonal projections to latent structures discriminant analysis (OPLS-DA). All the differential metabolites were listed, and proline and xanthosine were the two of the most downregulated metabolites by plasma treatment. By comprehensive metabolic pathway analysis with the KEGG pathway, we showed that alanine, aspartate, glutamate, and purine metabolism pathways were the most significantly suppressed after gas plasma treatment in human endothelial cells. Our finding gives an overall picture of the metabolic pathways affected by plasma treatment in endothelial cells.

UPLC-ESI-TOF MS-Based Metabolite Profiling of the Antioxidative Food Supplement Garcinia buchananii

2015 ◽  
Vol 63 (32) ◽  
pp. 7169-7179 ◽  
Author(s):  
Timo D. Stark ◽  
Sofie Lösch ◽  
Junichiro Wakamatsu ◽  
Onesmo B. Balemba ◽  
Oliver Frank ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Food Supplement ◽  
Garcinia Buchananii ◽  
Tof Ms

Metabolite profiling of Zi-Shen pill in rat biological specimens by UPLC-Q-TOF/MS

2015 ◽  
Vol 13 (2) ◽  
pp. 145-160 ◽  
Author(s):  
Xiao-Wei LIU ◽  
Feng ZHANG ◽  
Shou-Hong GAO ◽  
Bo JIANG ◽  
Wan-Sheng CHEN
Keyword(s):  
Metabolite Profiling ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document