Development of a rapid and improved two‐dimensional electrophoresis method for the separation of protein lysate

2019 ◽  
Vol 33 (12) ◽  
Author(s):  
Soon Hong Kwan ◽  
Mohd Nazri Ismail
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Electrophoresis Method ◽  
Dimensional Electrophoresis ◽  
Protein Lysate

Two-dimensional electrophoresis method used for determination of plasmid profiles of Escherichia coli isolated from a sewage treatment plant

1993 ◽  
Vol 39 (10) ◽  
pp. 990-993 ◽  
Author(s):  
M. Arturo ◽  
Z. Tamanai-Shacoori ◽  
C. Mamez ◽  
M. Pommepuy ◽  
M. Cormier
Keyword(s):  
Escherichia Coli ◽  
Brackish Water ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Treated Sewage ◽  
Electrophoresis Method ◽  
Dimensional Electrophoresis

The plasmid contents of 306 strains of Escherichia coli isolated from raw and treated sewage from a treatment plant as well as from the water 1 km downstream were determined. The number and molecular weight of plasmids isolated from a representative sample of these strains were also measured. It was observed that biological treatment did not significantly alter these parameters. In contrast, statistically significant differences in plasmid number and size were observed when strains from sewage (raw and treated) were compared with strains isolated from brackish water. In raw and treated sewage, more than 96% of the strains contained plasmids, compared with 85% in brackish water. Nine to 13% of the strains from sewage contained plasmids with more than 56 kilobases, while only 5% of the strains from brackish water reached this size.Key words: Escherichia coli, plasmids, two-dimensional electrophoresis, sewage treatment, brackish water.

scholarly journals Adaptation of the two-dimensional electrophoresis method for canned meat

2021 ◽  
Vol 854 (1) ◽  
pp. 012001
Author(s):  
V B Krylova ◽  
V T Gustova ◽  
A G Akhremko
Keyword(s):  
Molecular Weight ◽  
Two Dimensional ◽  
Molecular Weight Range ◽  
Two Dimensional Electrophoresis ◽  
Wide Range ◽  
Electrophoresis Method ◽  
Fat Removal ◽  
Canned Meat ◽  
Protein Components ◽  
Dimensional Electrophoresis

Abstract Studies of the qualitative indicators of canned meat in accordance with regulatory documents are carried out on average samples of specimens, but when studying by proteomic methods, such sampling does not allow high-quality separation of protein components due to the high fat content in the product. When two-dimensional electrophoresis was carried out on an average sample, fragments of the main muscle and connective tissue proteins of beef were found in small quantities, but the electrophoretogram was not very informative. A significantly better separation was achieved after removing the fat fraction from the product. When studying broth from canned meat, the largest amount of intensely coloured high-molecular-weight protein fractions with a mass of more than 50 kDa was revealed. The electrophoretogram of the meat pieces showed a wide range of proteins across the entire molecular weight range of the polyacrylamide gel, including major muscle proteins. The study of broth together with meat pieces but after fat removal is optimal for the primary screening of the protein component of canned meat.

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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