Two-dimensional electrophoresis method used for determination of plasmid profiles of Escherichia coli isolated from a sewage treatment plant

1993 ◽  
Vol 39 (10) ◽  
pp. 990-993 ◽  
Author(s):  
M. Arturo ◽  
Z. Tamanai-Shacoori ◽  
C. Mamez ◽  
M. Pommepuy ◽  
M. Cormier
Keyword(s):  
Escherichia Coli ◽  
Brackish Water ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Treated Sewage ◽  
Electrophoresis Method ◽  
Dimensional Electrophoresis

The plasmid contents of 306 strains of Escherichia coli isolated from raw and treated sewage from a treatment plant as well as from the water 1 km downstream were determined. The number and molecular weight of plasmids isolated from a representative sample of these strains were also measured. It was observed that biological treatment did not significantly alter these parameters. In contrast, statistically significant differences in plasmid number and size were observed when strains from sewage (raw and treated) were compared with strains isolated from brackish water. In raw and treated sewage, more than 96% of the strains contained plasmids, compared with 85% in brackish water. Nine to 13% of the strains from sewage contained plasmids with more than 56 kilobases, while only 5% of the strains from brackish water reached this size.Key words: Escherichia coli, plasmids, two-dimensional electrophoresis, sewage treatment, brackish water.

Changes of microbial indices of water quality in the Vistula and Brda rivers as a result of sewage treatment plant operation

2008 ◽  
Vol 37 (2) ◽  
Author(s):  
Maciej Walczak
Keyword(s):  
Water Quality ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Sewage Treatment Plants ◽  
Plant Operation ◽  
Treated Sewage ◽  
Microbiological Parameters ◽  
Microbial Indices

Changes of microbial indices of water quality in the Vistula and Brda rivers as a result of sewage treatment plant operationThis paper reports the results of studies of microbiological changes in the water quality of the Vistula and Brda rivers after the opening of sewage treatment plants in Bydgoszcz. The study involved determining the microbiological parameters of water quality. Based on the results obtained, it was found that the quality of the water in both rivers had improved decidedly after the opening of the plants, although an increased number of individual groups of microorganisms was found at the treated sewage outlet from one of the plants.

The Automatic Control System Based on PROFIBUS for the City Sewage Treatment

2013 ◽  
Vol 385-386 ◽  
pp. 857-861
Author(s):  
Xiao Ping Huang ◽  
Qi Xing Qing
Keyword(s):  
Control System ◽  
Automatic Control ◽  
Automatic Control System ◽  
Fuzzy Controller ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Difficult Problem ◽  
Urban Sewage ◽  
Treated Sewage

On the basis of the computerized technological process control of sewage treatment in a urban sewage treatment plant in the Zone of Nanning, the author had brought forward a plan of PROFIBUS that based on automatic control system for sewage treatment, and designed the automatic control system diagram and the PROFIBUS network diagram for sewage treatment plant. At the same time, the author also made a study of techniques to control the sewage aeration, which was the most difficult problem in sewage treatment process. The fuzzy controller could indicate the changes of the water quality in treated sewage, effectively controlled the volume of aeration and then cut down the operation costs,it had produced good economic and social benefits.

scholarly journals Clonal Dissemination of a CTX-M-15 β-Lactamase-Producing Escherichia coli Strain in the Paris Area, Tunis, and Bangui

2006 ◽  
Vol 50 (7) ◽  
pp. 2433-2438 ◽  
Author(s):  
M. Lavollay ◽  
K. Mamlouk ◽  
T. Frank ◽  
A. Akpabie ◽  
B. Burghoffer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Geographic Region ◽  
Coli Strain ◽  
E Coli ◽  
Paris Area ◽  
The Common ◽  
Repetitive Extragenic Palindromic Pcr

ABSTRACT One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR and pulsed-field gel electrophoresis. Various resistance profiles were identified among these clonal strains. After conjugation or electroporation of plasmids from E. coli strains representative of each profile and each geographic region, we observed seven resistance profiles in the recipient strains. Incompatibility typing showed that all the plasmids transferred from the clonal strains studied, except one, belonged to the incompatibility group FII. They all shared a multidrug resistance region (MDR) resembling the MDR region located in pC15-1a, a plasmid associated with an outbreak of a CTX-M-15-producing E. coli strain in Canada. They also shared the common backbone of an apparent mosaic plasmid, including several features present in pC15-1a and in pRSB107, a plasmid isolated from a sewage treatment plant. This study suggests that although the plasmid-borne bla CTX-M-15 gene could be transferred horizontally, its dissemination between France, Tunisia, and CAR was due primarily to its residence in an E. coli clone with a strong propensity for dissemination.

Identification of Escherichia coli ribosomal proteins by an alternative two-dimensional electrophoresis system

1988 ◽  
Vol 173 (2) ◽  
pp. 241-245 ◽  
Author(s):  
Dipak B. Datta ◽  
Li-Ming Changchien ◽  
Concepcion R. Nierras ◽  
William A. Strycharz ◽  
Gary R. Craven
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Electrophoresis System ◽  
Dimensional Electrophoresis
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