Simultaneous determination of 19 constituents in Cimicifugae Rhizoma by HPLC–DAD and screening for antioxidants through DPPH free radical scavenging assay

2019 ◽  
Vol 33 (10) ◽  
Author(s):  
Xueni Niu ◽  
Rulan Qin ◽  
Yudan Zhao ◽  
Ling Han ◽  
Jincai Lu ◽  
...  
Keyword(s):  
Free Radical ◽  
Simultaneous Determination ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

scholarly journals Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay

Data in Brief ◽  
2018 ◽  
Vol 17 ◽  
pp. 870-875 ◽  
Author(s):  
Kotaro Hara ◽  
Takao Someya ◽  
Katsura Sano ◽  
Yoshimasa Sagane ◽  
Toshihiro Watanabe ◽  
...  
Keyword(s):  
Free Radical ◽  
Sri Lanka ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

In Vitro Regeneration and Free Radical Scavenging Assay of Hypericum perforatum L.

2018 ◽  
Vol 42 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Mohammad Yaseen Mir ◽  
Azra N. Kamili ◽  
Qazi P. Hassan ◽  
Sabreena Rafi ◽  
Javid A. Parray ◽  
...  
Keyword(s):  
Free Radical ◽  
Hypericum Perforatum ◽  
In Vitro Regeneration ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypericum Perforatum L ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

scholarly journals BIOACTIVITY SCREENING OF SELECTED TRADITIONAL MEDICINAL PLANTS OF KERALA

2018 ◽  
Vol 11 (12) ◽  
pp. 339
Author(s):  
Alby Alphons Baby ◽  
Regi Raphael K
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Significant Activity ◽  
Traditional Use ◽  
Ic50 Value ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Objectives: Traditional medicines all over the world is revaluing nowadays by extensive research programs. To validate the traditional use, the active components in them need to be identified, characterized, and biologically evaluated. Stereospermum suaveolens, Hygrophila spinosa, and Naravelia zeylanica are important medicinal plants used by the ethnic people of Kerala against various ailments. The present study validates the ethnomedical uses of S. suaveolens, H. spinosa, and N. zeylanica by screening their antimicrobial, anthelmintic, and antioxidant properties.Method: All the analyses were done according to standard protocols.Results: The ethanolic extracts of their useful parts were investigated for antimicrobial activity against 10 human pathogenic microorganisms. All the three plants had shown prominent antimicrobial activities, and S. suaveolens exhibits comparatively more antifungal activity in their higher concentration (500 μg/mL). Anthelmintic efficiency of the plants was screened using Indian adult earthworm Pheretima posthuma. All of them had shown significant activity, and the highest was observed in S. suaveolens leaves. Antioxidant potential of the plants was screened using 2,2 diphenyl 1-picrylhydrazyl (DPPH) free radical scavenging assay and superoxide anion scavenging assay. In DPPH free radical scavenging assay, maximum radical scavenging was shown by S. suaveolens with IC50 value 61.6±2.3 μg/mL, and in superoxide anion scavenging assay, maximum activity was in N. zeylanica with IC50 value of 74.66±8.5 μg/mL.Conclusion: This study provides scientific evidence on the traditional use of S. suaveolens (leaves), H. spinosa (leaves), and N. zeylanica (aerial part) in treating microbial diseases, worm disturbances, and their potential as an antioxidant agent.

scholarly journals Antidiabetic and Antioxidant Activities of Decoctions of Coccinia grandis Linn. and Centella asiatica (L.) on Alloxan-induced Diabetic rats

2015 ◽  
Vol 17 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Md Siddiqul Islam ◽  
Salma Parvin ◽  
Md Nasir Uddin ◽  
Md Abdul Mazid
Keyword(s):  
Free Radical ◽  
Fasting Blood Glucose ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Diabetic Rats ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
High Dose ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

The decoctions of Coccinea grandis (L.) and Centella asiatica (L.) leaves were evaluated for their antidiabetic and antioxidant potentials in alloxan-induced rat model and by DPPH free radical scavenging assay, respectively. Graded doses of the decoctions administered to normal and experimental diabetic rats for two weeks showed significant reduction in fasting blood glucose levels in the normal as well as in treated diabetic animals. The experiment showed 188.42 ± 1.2 mg/dl glucose and 117.78 ± 11.2 mg/dl cholesterol levels for C. grandis, while 187.23 ± 0.8 mg/dl glucose and 116.14 ± 1.5 mg/dl cholesterol for C. asiatica. Although, the decoctions produced significant effect on serum urea level, the total protein and liver enzymes (SGOT and SGPT) were found to be normal. Acute toxicity studies showed that both the decoctions were safe at 2-fold high dose when compared with the commonly used antidiabetic dose. Total phenolic contents in decoctions were found to be 12.45±0.52 and 8.32 ± 0.31 for C. grandis and C. asiatica, respectively while DPPH free radical scavenging assay showed the IC50 values of 0.14 ± 0.001 for C. grandis and 0.17± 0.001 for C. asiatica. Our current studies for the first time, justified the use of decoctions of the leaves of C. grandis and C. asiatica for treating diabeties, hyperlipaedemia and atherogenic lesion in the indigenous system of medicine. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22322 Bangladesh Pharmaceutical Journal 17(1): 86-91, 2014

scholarly journals Nutritional Analysis and Determination of Antioxidant Activity Using Free Radical Scavenging Assay of Potatoes (Solanum tuberosum) from Two Regions of Pakistan

2017 ◽  
Vol 60 (2) ◽  
pp. 118-120
Author(s):  
Naseem Zahra ◽  
Muhammad Khalid Saeed ◽  
Ashi Nazir ◽  
Ijaz Ahmad ◽  
Alim-un- Nisa ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Free Radical Scavenging ◽  
Dependent Manner ◽  
Nutritional Analysis ◽  
Free Radical Scavenging Assay

Present study was designed to evaluate the nutritional values of red skin potatoes collected fromGujranwala and Lahore regions of Pakistan. Potatoes (Solanum tuberosum) of Gujranwala and Lahorewere found to contain moisture contents of 5.00 and 5.15%, respectively. Fat was 0.19% in S. tuberosumof Gujranwala whereas Lahore potatoes were found to contain fat 0.2%. Fibre content of S. tuberosum ofGujranwala was 16.47% while S. tuberosum of Lahore had 13.45% fibre. Protein contents of S. tuberosumof Gujranwala were 12.89% whereas for S. tuberosum of Lahore were 13.11%. The water extract showedsignificant free radical scavenging activities in DPPH radical scavenging antioxidant assay and antioxidantactivity was increased in a dose dependent manner. These results suggest that potato (S. tuberosum) is notonly a cheap source of very important nutritents but also has antioxidant activities which are helpful tomaintain different physiological functions of body.

scholarly journals Phytochemical and Pharmacological Evaluation of Cyperus odoratus Extract

2018 ◽  
Vol 21 (2) ◽  
pp. 150-159
Author(s):  
Ajijul Hakim Alif ◽  
Amir Hossain ◽  
Md Akbar Hossain ◽  
Tabitha Mitali Madhu ◽  
Salma Akter Sumi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Free Radical ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Free Radical Scavenging ◽  
Biological Data ◽  
Cytotoxic Activities ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Cyperus odoratus (Family: Cyperaceae) is a 10 to 50 cm long perennial herb used as traditional medicinal plant in Bangladesh to treat various pathological conditions like piles, tumour and wound. The ethanolic extract of C. odoratus (COET) has been evaluated for different bioactivities, for example, antioxidant, analgesic, anthelmintic and cytotoxic activities. Antioxidant activity was investigated by DPPH free radical scavenging assay, analgesic activity was evaluated from ‘acetic acid induced writhing inhibition test’ in mice, anthelmintic activities was tested by ‘time of paralysis and death’ assays and cytotoxic activities was observed from the ‘brine shrimp lethality bioassay’. Total tannin and phenol contents were also measured by using Folin-Ciocalteu reagent. Phytochemical screening indicated that the presence of alkaloids, tannins, flavonoids, glycosides, proteins, gums, steroids and acidic compounds. In DPPH free radical scavenging assay the extract showed IC50 value of 8.05 μg/ml, whereas the standard ascorbic acid showed 31.40 μg/ml. The total tannin and phenolic contents were found to be 3.35 and 1.68 mg GAE/100 g of dried extract, respectively. In acetic acid induced writhing assay, the extract showed 36.23 and 61.32% writhing inhibition at doses of 250 mg/kg and 500 mg/kg body weight, respectively, whereas standard Diclofenac-Na (25 mg/kg) showed 70.03% writhing inhibition. The plant extract showed anthelmintic activity in a dose dependant manner. COET also showed LD50 against brine shrimp nauplii at 11.344 μg/mL. After observing all preliminary biological data, it has been confirmed that the plant has antioxidant, analgesic, cytotoxic and anthelmintic properties.Bangladesh Pharmaceutical Journal 21(2): 150-159, 2018

Phytochemicals and Nutrient Profiles, Anti-oxidant Activity Study of Three Edible Parts (Flower, Fruit and Stem) of Musa paradisiaca

2019 ◽  
Vol 8 (1) ◽  
pp. 32-41
Author(s):  
Bikash Debnath ◽  
Kuntal Manna
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Banana Plant ◽  
Unripe Fruit ◽  
Musa Paradisiaca ◽  
Anti Oxidant ◽  
Edible Parts ◽  
Radical Scavenging Assay

Background:Musa paradisiaca (Banana plant), which belongs to the family of Musaceae, is a well-known herbaceous flowering edible plant. The flower, fruit, and stem part of the plant have been used for nutrients and health benefits.Objective:The aim of this study is to determine the secondary metabolites, proximate composition, minerals, heavy metals, and anti-oxidant activity of three edible parts (flower, unripe fruit, and stem) of Musa paradisiaca.Methods:The content of alkaloid and tannin was determined by simple titrimetric method and colorimetric method was used for the determination of the content of phenol and flavonoid. Association of Official Analytical Chemicals (AOAC) method was used for the determination of proximate composition and the content of trace elements was analyzed by using atomic absorption spectrophotometer. The anti-oxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and hydrogen peroxide (H2O2) radical scavenging assay.Results:The results indicated that three edible parts (flower, unripe fruit, and stem) of the banana plant contained a good amount of secondary metabolites (such as alkaloid, phenol, flavonoid, and tannin) and also primary metabolites (such as carbohydrate, protein, and fat). Banana fruit contained a high amount of energy (261.31 kcal/100g) compared to the other two parts (flower, and stem). Iron, potassium, phosphorus, calcium were present in these three edible parts of the banana plant. Lead was found in negligible amount and arsenic was not detected. Fifty percent of ethanolic extract of three edible parts of the banana plant showed significant DPPH free radical scavenging and H202 radical scavenging activity as compared to standard ascorbic acid.Conclusion:Based on these findings, three edible parts of Musa paradisiaca may be recommended as a good source of nutrients.

Sign in / Sign up

Export Citation Format

Share Document