Simultaneous determination of 20(S )-protopanaxadiol and its three metabolites in rat plasma by LC-MS/MS: application to their pharmacokinetic studies

2018 ◽  
Vol 32 (8) ◽  
pp. e4252 ◽  
Author(s):  
Jin-Qi Li ◽  
Jia-Feng Wang ◽  
Jie Li ◽  
Shu-han Zhang ◽  
Dan He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Pharmacokinetic Studies

A HPLC-DAD-MS/MS Method for Simultaneous Determination of Six Active Ingredients of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection in Rat Plasma and its Application to Pharmacokinetic Studies

2020 ◽  
Vol 21 ◽  
Author(s):  
Jianyang Pan ◽  
Luquan Zhang ◽  
Difeifei Xiong ◽  
Bailing Li ◽  
Haibin Qu
Keyword(s):  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Array Detector ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Active Ingredients ◽  
Salviae Miltiorrhizae ◽  
Salvianolic Acids

Aims: Pharmacokinetic Study of Salviae Miltiorrhizaeand Ligustrazine Hydrochloride injection. For the evaluation of mechanism of action, safety and clinical rational use of Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection. Background: Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection is a compound preparation consisted of Salvia Miltiorrhiza extract and ligustrazine hydrochloride for the treatment of cardiovascular and cerebrovascular diseases in China. Methods: Plasma samples were precipitated with methanol, which was spiked with ascorbic acid and the supernatant was separated on a Waters Cortecs C18 column, by using a gradient mobile phase system of acetonitrile-water containing 0.05% formic acid (v/v). For internal standards, puerarin was selected for the five salvianolic acids, while isofraxidin was used for ligustrazine hydrochloride. Besides, electrospray ionization in negative mode and multiple-reaction monitoring were used to identify and quantify the five salvianolic acids, whereas ligustrazine hydrochloride was quantified at 310 nm using the diode array detector. Results: Noticeably, all calibration curves showed good linearity (R2>0.99) over the concentration range, with a lower limit of quantification between 0.00411 and 0.0369μg/mL for salvianolic acids, and 1.74 μg/mL for ligustrazine hydrochloride. Next, the precision of the developed method was evaluated by intra-and inter-day assays, and the percentage of relative standard deviation was within 10%. Although the extraction efficiency of some salvianolic acids were not very satisfactory, the sensitivity of the analytical method met the analysis requirements of rat plasma samples. Moreover, the validated method was successfully applied to a pharmacokinetic study of Salviae Miltiorrhizae and Ligustrazine Hydrochloride injection in the rat model. Conclusion: Linear pharmacokinetic characteristics were observed for the six active ingredients after intravenous infusion administration in rats, within the dose range examined here. In summary, our study proposed a HPLC-DAD-MS/MS method in the simultaneous determination of multiple ingredients, and demonstrated its applicability in pharmacokinetic studies.

simultaneous determination of mycophenolic acid and its metabolites by HPLC and pharmacokinetic studies in rat plasma and bile

2011 ◽  
Vol 34 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Jun-wei Gao ◽  
Zhi-hai Peng ◽  
Xiao-yu Li ◽  
Bo Sun ◽  
Yan-kun Guo ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Mycophenolic Acid ◽  
Rat Plasma ◽  
Pharmacokinetic Studies
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