Geographical classification ofEpimediumbased on HPLC fingerprint analysis combined with multi-ingredients quantitative analysis

2016 ◽  
Vol 31 (5) ◽  
pp. e3871 ◽  
Author(s):  
Ning Xu ◽  
Guofu Zhou ◽  
Xiaojuan Li ◽  
Heng Lu ◽  
Fanyun Meng ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Fingerprint Analysis ◽  
Hplc Fingerprint

scholarly journals A Strategy for Quality Control of Vespa magnifica (Smith) Venom Based on HPLC Fingerprint Analysis and Multi-Component Separation Combined with Quantitative Analysis

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2920 ◽  
Author(s):  
Si-Tong Zhou ◽  
Kai Luan ◽  
Lian-Li Ni ◽  
Ying Wang ◽  
Shi-Meng Yuan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Principal Component ◽  
Good Precision ◽  
Fingerprint Analysis ◽  
Wasp Venom ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Relative Standard

As a folk medicine of the Jingpo minority in Yunnan province, the venom of Vespa magnifica has been commonly used for the treatment of rheumatoid arthritis. Quality standardization of the wasp venom is a necessary step for its pharmaceutical research and development. To control the quality of the wasp venom, a method based on high-performance liquid chromatography (HPLC) was developed for chemical fingerprint analysis. In the chromatographic fingerprinting, chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA), were applied to classify 134 batches (S1–S134) of wasp venom from different origins. The HPLC fingerprint method displayed good precision (Relative standard deviation, RSD < 0.27%), stability (in 16 h, RSD < 0.34%), and repeatability (RSD < 1.00%). Simultaneously, four compounds (VMS1, VMS2, VMS3, and VMS4) in the wasp venom were purified and identified. VMS1 was 5-hydroxytryptamine, and the other compounds were three peptides that were sequenced as follows: Gly–Arg–Pro–Hyp–Gly–Phe–Ser–Pro–Phe–Arg–Ile–Asp–NH2 (VMS2), Ile–Asn–Leu–Lys–Ala–Ile–Ala–Ala–Leu–Ala–Lys–Lys–Leu–Leu–NH2 (VMS3), and Phe–Leu–Pro–Ile–Ile–Gly–Lys–Leu–Leu–Ser–Gly–Leu–Leu–NH2 (VMS4). The quantifications for these components were 110.2 mg/g, 26.9 mg/g, 216.3 mg/g, and 58.0 mg/g, respectively. The results of this work indicated that the combination of the chemical fingerprint and quantitative analysis offers a reasonable way to evaluate the quality of wasp venom.

scholarly journals Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7242
Author(s):  
Hongmei Sun ◽  
Yini Cai ◽  
Jie Shen ◽  
Enyao Ma ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Mobility ◽  
High Performance ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

Combinative method using multi-components quantitation by single reference standard and HPLC fingerprint for comprehensive evaluation of Rhodiola crenulata H.Ohba

2014 ◽  
Vol 6 (15) ◽  
pp. 5891-5898 ◽  
Author(s):  
Yiyang Du ◽  
Qing Li ◽  
Jingjing Liu ◽  
Yidi Yin ◽  
Kaishun Bi
Keyword(s):  
Quantitative Analysis ◽  
Comprehensive Evaluation ◽  
Reference Standard ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Hplc Fingerprint ◽  
Rhodiola Crenulata ◽  
Different Origins

HPLC-DAD quantitative analysis and chromatographic fingerprint analysis were developed to evaluate the quality of Rhodiola crenulata H.Ohba from different origins.

scholarly journals Compound Traditional Chinese Medicine Dermatitis Ointment Ameliorates Inflammatory Responses and Dysregulation of Itch-Related Molecules in AD

2021 ◽  
Author(s):  
Rongrong Zhang ◽  
Shuai Shao ◽  
Yingxin Shen ◽  
Jiaming Sun ◽  
Songlan Piao ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Inflammatory Responses ◽  
Dependent Manner ◽  
Inflammatory Skin Disease ◽  
Fingerprint Analysis ◽  
Hplc Fingerprint ◽  
Effective Components ◽  
Anti Inflammatory ◽  
Dose Dependent

Abstract Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease accompanied by an itchy and scaly rash. Compound traditional Chinese medicine dermatitis ointment (CTCMDO) is a traditional classics aimed at AD composed of a mixture of extracts from five plants known to have anti-inflammatory and antiallergic effects. Materials and methods: In this study, we used HPLC and LC/MS to analyze the effective components of CTCMDO in detail and establish its HPLC fingerprint analysis. On this basis, this article studied the anti-inflammatory and antipruritic activities of CTCMDO in the treatment of DNCB-induced AD in mice.Results: Through comparison with literature data, a total of 43 compounds were identified, including phenylpropionic acid compounds; alkaloid compounds; curcumin compounds and lignans. On this basis, a fingerprint with 17 common peaks was established. In AD-like mice, CTCMDO treatment suppressed the scratching behavior induced by DNCB in a dose-dependent manner and inhibited the production of Th1/2 cytokines in serum. CTCMDO treatment reversed the up regulation of P substance levels of itch-related genes in the skin. Furthermore, CTCMDO suppressed the phosphorylation of JNK、ERK and p38 in the skin. Conclusion: In all, our work indicated that CTCMDO can signifificantly attenuate the pathological alterations of Th1/2 cytokines and itch-related mediators, and inhibit the phosphorylation of MAPKs to treat AD.

scholarly journals Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1521 ◽  
Author(s):  
Yehong Li ◽  
Yiming Zhang ◽  
Zejun Zhang ◽  
Yupiao Hu ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Evaluation Method ◽  
Herbal Medicines ◽  
Assessment Method ◽  
Gastrodia Elata ◽  
Clinical Safety ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Identification of three Daphne species by DNA barcoding and HPLC fingerprint analysis

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0201711 ◽  
Author(s):  
Yanpeng Li ◽  
Lu Geng ◽  
Yuanyan Liu ◽  
Mingyang Chen ◽  
Qirui Mu ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Fingerprint Analysis ◽  
Hplc Fingerprint
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