Development and validation of an RP-HPLC method for the quantitation of Orteronel (TAK-700), a CYP17A1 enzyme inhibitor, in rat plasma and its application to a pharmacokinetic study

2013 ◽  
Vol 27 (12) ◽  
pp. 1590-1594 ◽  
Author(s):  
Avinash Kumar ◽  
Vijay Kumar S ◽  
Sandip Gurav ◽  
Mohd Zainuddin ◽  
Purushottam Dewang ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Enzyme Inhibitor ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc ◽  
Development And Validation

Development and validation of a RP-HPLC method for the quantitation of tofacitinib in rat plasma and its application to a pharmacokinetic study

2015 ◽  
Vol 29 (9) ◽  
pp. 1325-1329 ◽  
Author(s):  
Vijay Kumar S ◽  
Vinay Dhiman ◽  
Kalpesh Kumar Giri ◽  
Kuldeep Sharma ◽  
Mohd Zainuddin ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc ◽  
Development And Validation

Ecofriendly chromatographic methods for determination of co-prescribed drugs, olanzapine and metformin, in rat plasma

Bioanalysis ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 597-613
Author(s):  
Aml A Emam ◽  
Neven M Habib ◽  
Hamada M Mahmoud ◽  
Nada S Abdelwhab ◽  
Maha M Abdelrahman
Keyword(s):  
Pharmaceutical Preparation ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hypoglycemic Agent ◽  
Chromatographic Methods ◽  
Rp Hplc ◽  
Development And Validation ◽  
Developing System

Background: Olanzapine (OLZ) is one of most recommended drugs for the treatment of schizophrenia while metformin (MET) is the most commonly used hypoglycemic agent. Aim: Development and validation of two green, sensitive and accurate chromatographic methods for the simultaneous determination of OLZ along with the co-prescribed, MET. Materials & methods: TLC-densitometric method with a developing system consisting of methylene chloride:methanol:ethyl acetate:triethylamine (4:4:5:0.1, by volume) and a reversed-phase (RP)-HPLC method where the chromatographic separation was performed using ethanol:water mixture (50: 50, v/v) as a mobile phase. Results: TLC-densitometric method had linearity over concentration ranges of 160–4000 ng/band for OLZ and 150–4500 ng/band for MET, while RP-HPLC method was linear and validated over concentration range of 300–20000 ng/ml for OLZ and MET. Conclusion: Pharmacokinetic study was successfully performed and suggested the possibility of co-administration of MET with OLZ and their further formulation in one pharmaceutical preparation to enhance patient’s compliance.

A rapid and simple RP-HPLC method for quantification of kirenol in rat plasma after oral administration and its application to pharmacokinetic study

2010 ◽  
Vol 25 (5) ◽  
pp. 542-546 ◽  
Author(s):  
Xiao-Li Song ◽  
Qing-Ying Zhang ◽  
Zhe-Ming Wang ◽  
Hong-Zheng Fu ◽  
Rui-Qin Qian
Keyword(s):  
Oral Administration ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

DEVELOPMENT AND VALIDATION OF NEW RP–HPLC METHOD FOR DETERMINATION OF BESIFLOXACIN IN ANIMAL MODEL

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (01) ◽  
pp. 26-32
Author(s):  
A Singh ◽  
◽  
C. L Singh ◽  
S. Kumar ◽  
M. Kumar
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Standard Curve ◽  
Absorbance Detection ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Development And Validation

A sensitive and accurate reversed– phase high performance liquid chromatography (RP-HPLC) method with UV absorbance detection at 289 nm was developed and validated for the determination and quantification of besifloxacin (BSF) in rat plasma. Ofloxacin was used as an internal standard (IS). The sample was prepared by liquid extraction of BSF from plasma, using methanol and acetonitrile (70:30). The chromatographic separation was achieved with octadecylsilane (ODS-3), Hypersil® C18 column (250 mm×6mm×5μm). The chromatographic runtime was less than 5 minutes where the retention time of internal standard and the drug was 2.15 min and 3.30 min respectively. A standard curve with a regression coefficient (r2) 0.999 was obtained in the range of 0.025-20 μg/mL. The method was validated with respect to linearity, range, precision, accuracy and robustness according to ICH guidelines. The method was found to be accurate and robust with a runtime of less than 5 minutes. Hence, the present method was rapid and economical to use for clinical studies as well as to analyze the drug in different plasma samples.

A simplified and sensitive validated RP-HPLC method for determination of temozolomide in rat plasma and its application to a pharmacokinetic study

2018 ◽  
Vol 41 (10) ◽  
pp. 692-697 ◽  
Author(s):  
Archana Khosa ◽  
Kowthavarapu V. Krishna ◽  
Ranendra N. Saha ◽  
Sunil K. Dubey ◽  
Satish Reddi
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

Development and validation of RP-HPLC method for estimation of fisetin in rat plasma

2020 ◽  
Author(s):  
Rajan Kumar ◽  
Rakesh Kumar ◽  
Rubiya Khursheed ◽  
Ankit Awasthi ◽  
Navneet Khurana ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc ◽  
Development And Validation

scholarly journals Development and validation of RP-HPLC method for sildenafil citrate in rat plasma-application to pharmacokinetic studies

2013 ◽  
Vol 21 (3) ◽  
pp. 317-321 ◽  
Author(s):  
A.S. Tripathi ◽  
I. Sheikh ◽  
A.P. Dewani ◽  
P.G. Shelke ◽  
R.L. Bakal ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Sildenafil Citrate ◽  
Pharmacokinetic Studies ◽  
Rp Hplc ◽  
Development And Validation

Development and validation of an RP-HPLC method for the quantitation of odanacatib in rat and human plasma and its application to a pharmacokinetic study

2015 ◽  
Vol 29 (11) ◽  
pp. 1664-1669
Author(s):  
Anitha Police ◽  
Sandip Gurav ◽  
Vinay Dhiman ◽  
Mohd Zainuddin ◽  
Ravi kanth Bhamidipati ◽  
...  
Keyword(s):  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Hplc Method ◽  
Rp Hplc ◽  
Development And Validation
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