Background:
Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties.
Objective:
In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma.
Methods:
Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in
2 mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model.
Results:
Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to
90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500 ng/mL. The inter-day
and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%.
Conclusion:
The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.
A Highly Sensitive and Rugged LC-MS/MS Method Development and Validation for Determination of Elvitegravir in Rat Plasma: Application to Pharmacokinetic Study