Development and validation of a highly sensitive LC–ESI-MS/MS method for the determination of Orteronel® (TAK-700) in rat plasma: application to a pharmacokinetic study

Bioanalysis ◽  
2012 ◽  
Vol 4 (12) ◽  
pp. 1471-1480 ◽  
Author(s):  
Sandip Gurav ◽  
Anitha Police ◽  
Mohd Zainuddin ◽  
Junaid Hassan Farooqui ◽  
Krishna Reddy G ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Esi Ms ◽  
Highly Sensitive ◽  
Development And Validation

Development and validation of a highly sensitive LC-MS/MS-ESI method for the determination of nobiletin in rat plasma: application to a pharmacokinetic study

2012 ◽  
Vol 26 (12) ◽  
pp. 1464-1471 ◽  
Author(s):  
Avinash Kumar ◽  
V. C. Devaraj ◽  
Kalpesh C. Giri ◽  
Sanjeev Giri ◽  
Sriram Rajagopal ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Highly Sensitive ◽  
Development And Validation

Enantioselective LC‐ESI‐MS/MS determination of dropropizine enantiomers in rat plasma and application to a pharmacokinetic study

2018 ◽  
Vol 33 (3) ◽  
pp. e4434 ◽  
Author(s):  
Narayanan Balaji ◽  
Bhavesh Babulal Gabani ◽  
Umesh Todmal ◽  
Suresh P. Sulochana ◽  
Neeraj Kumar Saini ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Esi Ms

scholarly journals Development and Validation of UPLC-ESI-MS/MS Technique for the Determination of 2-Isopropyl-4-(chloromethyl)thiazole in Ritonavir

2020 ◽  
Vol 32 (7) ◽  
pp. 1733-1740
Author(s):  
K. Durga Raja ◽  
V. Saradhi Venkata Ramana ◽  
K. Raghu Babu ◽  
B. Kishore Babu ◽  
V. Jagadeesh Kumar ◽  
...  
Keyword(s):  
High Strength ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Internal Diameter ◽  
Esi Ms ◽  
Quantitation Limit ◽  
Highly Sensitive ◽  
Liquid Chromatography Tandem Mass ◽  
Development And Validation

The objective of this work was to develop and validate a rapid, highly sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS) method for the quantification of 2-isopropyl-4-(chloromethyl)thiazole in ritonavir. Chromatographic conditions of this impurity were achieved on an AQUITY UPLC column HSS (high strength silica) T3 column (100 mm long, 2.1 mm internal diameter, 1.8 μm diameter) using a gradient elution with 0.1% formic acid in water and methanol at a flow rate of 0.3 mL/min. LCMS/MS was operated under the multiple reaction mode (MRM) using electrospray ionization technique in positive ion mode and the transitions of m/z 176.1[M+H]+→140.1 for quantifier, 176.1[M+H]+→71.0 for qualifier were used to measure the impurity, respectively. The total chromatographic run time was 10 min. Full validation of the analytical method was carried out, including its system precision, selectivity, linearity, accuracy, recovery, ruggedness, stability and robustness. A linear response function was achieved in the concentration range of 0.12-1.86 μg/g with r > 0.99. The detection limit and quantitation limit of this impurity were 0.06 and 0.12 μg/g, respectively. Consistent recoveries were obtained during intra- and inter-day precision experiments in validation ranged from 80-120%. The developed method could be helpful not only for quality control and also for risk management of potential genotoxicity of this impurity in ritonavir drug substance.

Development and validation of a LC-ESI–MS/MS method for the determination of swertiamarin in rat plasma and its application in pharmacokinetics

2011 ◽  
Vol 879 (19) ◽  
pp. 1653-1658 ◽  
Author(s):  
Hong-Liang Li ◽  
Xiao-Jin Peng ◽  
Jian-Chang He ◽  
En-Fu Feng ◽  
Gui-Li Xu ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Esi Ms ◽  
Development And Validation

Simultaneous determination of esculin and its metabolite esculetin in rat plasma by LC–ESI-MS/MS and its application in pharmacokinetic study

2012 ◽  
Vol 907 ◽  
pp. 27-33 ◽  
Author(s):  
Ying-yi Li ◽  
Ye-ying Song ◽  
Chang-hui Liu ◽  
Xiao-tao Huang ◽  
Xia Zheng ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Esi Ms
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