Direct enantiomeric TLC resolution of dl-penicillamine using (R)-mandelic acid and l-tartaric acid as chiral impregnating reagents and as chiral mobile phase additive

2008 ◽  
Vol 22 (11) ◽  
pp. 1237-1242 ◽  
Author(s):  
Ravi Bhushan ◽  
Charu Agarwal
Keyword(s):  
Tartaric Acid ◽  
Mobile Phase ◽  
Mandelic Acid ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive

scholarly journals Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yangfeng Peng ◽  
Quan Sophia He ◽  
Jiang Cai
Keyword(s):  
Mobile Phase ◽  
Ph Value ◽  
Limit Of Detection ◽  
Dihydrogen Phosphate ◽  
Column Temperature ◽  
Aqueous Buffer ◽  
Rp Hplc ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive ◽  
Limit Of Quantitation

Enantiomeric separation of citalopram (CIT) was developed using a reversed phase HPLC (RP-HPLC) with sulfobutylether-β-cyclodextrin (SBE-β-CD) as a chiral mobile phase additive. The effects of the pH value of aqueous buffer, concentration of chiral additive, composition of mobile phase, and column temperature on the enantioseparation of CIT were investigated on the Hedera ODS-2 C18column (250 mm × 4.6 mm × 5.0 um). A satisfactory resolution was achieved at 25°C using a mobile phase consisting of a mixture of aqueous buffer (pH of 2.5, 5 mM sodium dihydrogen phosphate, and 12 mM SBE-β-CD), methanol, and acetonitrile with a volumetric ratio of 21 : 3 : 1 and flow rate of 1.0 mL/min. This analytical method was evaluated by examining the precision (lower than 3.0%), linearity (regression coefficients close to 1), limit of detection (0.070 µg/mL for (R)-CIT and 0.076 µg/mL for (S)-CIT), and limit of quantitation (0.235 µg/mL for (R)-CIT and 0.254 µg/mL for (S)-CIT).

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