A laboratory work to introduce biochemistry undergraduate students to basic enzyme kinetics-alkaline phosphatase as a model

2018 ◽  
Vol 47 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Johanna G. Miquet ◽  
Lorena González ◽  
Ana I. Sotelo ◽  
Rodolfo M. González Lebrero
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Kinetics ◽  
Undergraduate Students ◽  
Laboratory Work

PRÁTICA LABORATORIAL DE PESQUISA SEDIMENTOLÓGICA

2019 ◽  
Vol 21 (2) ◽  
pp. 218-226
Author(s):  
Alyne Karollayne Melquiades Souza Silva ◽  
Joanderson Fernandes Simões ◽  
Júlio César Reis Silva ◽  
Amanda Cristina Dias Lima ◽  
Marcelo Santos Chaves ◽  
...  
Keyword(s):  
Undergraduate Students ◽  
Experimental Research ◽  
Laboratory Experiments ◽  
Rio Grande ◽  
Physical Geography ◽  
Laboratory Work ◽  
Theoretical Knowledge ◽  
Laboratory Experience

Esta pesquisa teve como finalidade relatar experiências em laboratório, especificamente o Laboratório de Geografia Física - Pesquisa (LabGeoFis-Pesquisa), localizado no Departamento de Geografia, no Centro de Ciências Humanas, Letras e Artes, da Universidade Federal do Rio Grande do Norte. Procuramos relatar, de forma concisa, as principais técnicas aplicadas ao trabalho laboratorial, enfatizado a temática da Geografia Física. Para tecer essas considerações, nos fundamentamos em autores como Venturi (2005) e Rudio (1990), os quais tratam sobre teorias e procedimentos relacionados às práticas de laboratório e a pesquisa descritiva e experimental, respectivamente. Para realizar esta pesquisa, levamos em consideração os trabalhos e as experiências desenvolvidas pelos alunos de bacharelado e licenciatura do primeiro período do curso de Geografia. Os resultados aqui obtidos, nos mostraram a importância dos conhecimentos teóricos aliados com a prática de laboratório.Palavras chaves: Laboratório; Experiência; Técnicas. ABSTRACT This research had the purpose of reporting laboratory experiments, specifically the Laboratory of Physical Geography - Research (LabGeoFis-Search), located in the Department of Geography, at the Humanities, Letters and Arts Center of the Federal University of Rio Grande do Norte. We try to report, in a concise way, the main techniques applied to laboratory work, emphasizing the theme of Physical Geography. In order to make these considerations, we base ourselves on authors such as Venturi (2005) and Rudio (1990), who deal with theories and procedures related to laboratory practices and descriptive and experimental research, respectively. To carry out this research, we take into account the work and experiences developed by the baccalaureate and undergraduate students of the first period of the Geography course. The results obtained here showed us the importance of theoretical knowledge allied with laboratory practice.Keywords: Laboratory; Experience; Technique. RESUMENEl propósito de esta investigación fue reportar experimentos de laboratorio, específicamente el Laboratorio de Investigación de Geografía Física (LabGeoFis-Pesquisa), ubicado en el Departamento de Geografía, en el Centro de Humanidades, Letras y Artes, Universidad Federal de Río Grande del Norte.

scholarly journals Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.

1978 ◽  
Vol 26 (10) ◽  
pp. 792-802 ◽  
Author(s):  
N S Markovic ◽  
L E Lipkin ◽  
O S Markovic ◽  
M J Wade
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzyme Kinetics ◽  
Single Cell ◽  
Quantitative Data ◽  
Single Cells ◽  
Final Reaction Product ◽  
Chamber Studies ◽  
Kinetics Of ◽  
Quantitative Cytochemistry

A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.

scholarly journals Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

Science ◽  
2021 ◽  
Vol 373 (6553) ◽  
pp. eabf8761
Author(s):  
C. J. Markin ◽  
D. A. Mokhtari ◽  
F. Sunden ◽  
M. J. Appel ◽  
E. Akiva ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Kinetics ◽  
High Throughput ◽  
Active Site ◽  
Molecular Mechanisms ◽  
Extensive Investigation ◽  
Purification And Characterization ◽  
Multiple Substrates ◽  
Catalytic Effects

Systematic and extensive investigation of enzymes is needed to understand their extraordinary efficiency and meet current challenges in medicine and engineering. We present HT-MEK (High-Throughput Microfluidic Enzyme Kinetics), a microfluidic platform for high-throughput expression, purification, and characterization of more than 1500 enzyme variants per experiment. For 1036 mutants of the alkaline phosphatase PafA (phosphate-irrepressible alkaline phosphatase of Flavobacterium), we performed more than 670,000 reactions and determined more than 5000 kinetic and physical constants for multiple substrates and inhibitors. We uncovered extensive kinetic partitioning to a misfolded state and isolated catalytic effects, revealing spatially contiguous regions of residues linked to particular aspects of function. Regions included active-site proximal residues but extended to the enzyme surface, providing a map of underlying architecture not possible to derive from existing approaches. HT-MEK has applications that range from understanding molecular mechanisms to medicine, engineering, and design.

Developing an Online Simulation to Teach Enzyme Kinetics to Undergraduate Biochemistry Students

2020 ◽  
pp. 281-302
Author(s):  
Maurizio Costabile ◽  
Hayley Timms
Keyword(s):  
Enzyme Kinetics ◽  
Undergraduate Students ◽  
South Australia ◽  
Teaching Staff ◽  
Interactive Simulation ◽  
Online Simulation ◽  
Scientific Principles ◽  
Alternative Approaches ◽  
The University ◽  
Enhance Student Learning

One approach used in teaching scientific principles is laboratory practical classes. However, it can be challenging to teach concepts prior to their introduction in lectures. Academic teaching staff that wish to use alternative approaches to bridge this gap and, in turn, enhance student learning, often require help from their local Educational Developers (EDs). This chapter outlines the process of identifying a problem and then developing, implementing, and evaluating an online interactive simulation to teach enzyme kinetics to undergraduate students at the University of South Australia (UniSA). The challenges faced by the academic and ED in developing the simulation are covered. By the end of the chapter, the reader (academic or ED) will have a better appreciation of the challenges faced in developing a new teaching approach as well as the strategies that can be used to address these challenges.

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