Characterization of HIV‐1 virus‐like particles and determination of Gag stoichiometry for different production platforms

2021 ◽  
Author(s):  
Jesús Lavado‐García ◽  
Inmaculada Jorge ◽  
Arnau Boix‐Besora ◽  
Jesús Vázquez ◽  
Francesc Gòdia ◽  
...  
Keyword(s):  
Virus Like Particles ◽  
Hiv 1

Production and characterization of HIV-1 virus-like particles using transient gene expression in mammalian cells

2014 ◽  
Vol 31 ◽  
pp. S42
Author(s):  
Sonia Gutiérrez-Granados ◽  
Laura Cervera ◽  
Segura Maria de las Mercedes ◽  
Francesc Gòdia
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Transient Gene Expression ◽  
Virus Like Particles ◽  
Expression In Mammalian Cells ◽  
Hiv 1

scholarly journals Immunological Characterization of Plant-Based HIV-1 Gag/Dgp41 Virus-Like Particles

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151842 ◽  
Author(s):  
Sarah A. Kessans ◽  
Mark D. Linhart ◽  
Lydia R. Meador ◽  
Jacquelyn Kilbourne ◽  
Brenda G. Hogue ◽  
...  
Keyword(s):  
Immunological Characterization ◽  
Virus Like Particles ◽  
Hiv 1

scholarly journals Biological and biochemical characterization of HIV ‐1 Gag/dgp41 virus‐like particles expressed in N icotiana benthamiana

2013 ◽  
Vol 11 (6) ◽  
pp. 681-690 ◽  
Author(s):  
Sarah A. Kessans ◽  
Mark D. Linhart ◽  
Nobuyuki Matoba ◽  
Tsafrir Mor
Keyword(s):  
Biochemical Characterization ◽  
Virus Like Particles ◽  
Hiv 1

In-silico evaluation of ‘Mirror Repeats’ In HIV Genome

2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Yadav Sandeep ◽  
Yadav Usha ◽  
Sharma Dinesh C.
Keyword(s):  
Neurological Disorders ◽  
Repetitive Sequences ◽  
Viral Integration ◽  
Triplex Dna ◽  
Repeat Sequences ◽  
Eukaryotic Organisms ◽  
Further Development ◽  
Hiv 1

The repetitive sequences played an important role in the characterization of both prokaryotic & eukaryotic organisms. Various different patterns of repetitive sequences have also been identified in organisms. Among all the repeat sequences. Mirror Repeats (MR`s) play an important role in various types of neurological disorders. These MR`s have also been reported for structure determination of genomes, triplex DNA formation & various other genome functions. We have followed a distinguished method referred to as FPCB (FASTA PARALLEL COMPLEMENT BLAST) for the identification of MR`s. The above said method used to identify MR’s in both types of HIV viruses (HIV-1 & HIV-2). Present investigation reported that MR’s are frequently distributed in all the regions of the genomes of both types. As a result, 232 & 248 total numbers of MR`s identified in both the HIV-1 & HIV-2 genome respectively. In addition, it was also revealed that the majority of the identified sequences are imperfect. The maximum length of MR`s in HIV-1 is of 47 nucleotides (NTD`s), however in case of HIV-2, it is of 49 nucleotides (NTD`s). Present investigation will be helpful for further development of a link between mirror repeats and host genome, which will be a new trend to block the viral integration as well as pathogenicity.

Characterization of Impressed Current Technique to Model Corrosion of Reinforcement in Concrete

2020 ◽  
Vol 11 (1) ◽  
pp. 93-99
Author(s):  
Abu Zakir Morshed ◽  
Sheikh Shakib ◽  
Tanzim Jahin
Keyword(s):  
Immersion Time ◽  
Chloride Content ◽  
Engineering Science ◽  
Coastal Regions ◽  
Faraday’S Law ◽  
Current Technique ◽  
Corrosion Cell ◽  
Impressed Current

Corrosion of reinforcement is an important durability concern for the structures exposed to coastal regions. Since corrosion of reinforcement involves long periods of time, impressed current technique is usually used to accelerate the corrosion of reinforcement in laboratories. Characterization of impressed current technique was the main focus of this research,which involved determination of optimum chloride content and minimum immersion time of specimens for which the application of Faraday’s law could be efficient. To obtain optimum chloride content, the electrolytes in the corrosion cell were prepared similar to that of concrete pore solutions. Concrete prisms of 200 mm by 200 mm by 300 mm were used to determine the minimum immersion time for saturation. It was found that the optimum chloride content was 35 gm/L and the minimum immersion time for saturation was 140 hours. Accounting the results, a modified expression based on Faraday’s law was proposed to calculate weight loss due to corrosion. Journal of Engineering Science 11(1), 2020, 93-99

The Determination of Sense via Deleuze and Blanchot: Paradoxes of the Habitual, the Immemorial, and the Eternal Return

2008 ◽  
Vol 2 (2) ◽  
pp. 155-177 ◽  
Author(s):  
Eugene Brently Young
Keyword(s):  
Eternal Return

Eternal return is the paradox that accounts for the interplay between difference and repetition, a dynamic at the heart of Deleuze's philosophy, and Blanchot's approach to this paradox, even and especially through what it elides, further illuminates it. Deleuze draws on Blanchot's characterisations of difference, forgetting, and the unlivable to depict the ‘sense’ produced via eternal return, which, for Blanchot, is where repetition implicates or ‘carries’ pure difference. However, for Deleuze, difference and the unlivable are also developed by the living repetition or ‘contraction’ of habit, which results in his distinctive characterization of ‘force’, ‘levity’, and sense in eternal return.

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